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mesoSPIM_supported_hardware
Nikita Vladimirov edited this page Dec 9, 2022
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The mesoSPIM software is compatible with various hardware, and your particular system can be defined using the config file. In other words, the hardware can be added, replaced or upgraded in a modular way. See config file examples.
Model | FOV: W x H, diagonal, mm | Pixel array | Pixel size, |
Megapixels | Status | Notes | mesoSPIM version |
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Hamamatsu Orca 4.0v3 | 13.312 x 13.312, diag. 18.8 | 2048 x 2048 | 6.5 | 4.2 MP | Supported and recommended | Use the version with PCIe frame grabber, not the USB3 (the latter is too slow) | v4-5 |
Hamamatsu Orca Fusion | 14.976 x 14.976, diag. 21.2 | 2304 x 2304 | 6.5 | 5.3 MP | Tested, stable | Use CoaXPress frame grabber version, NOT the USB3. | v4-5 |
Teledyne Photometrix Kinetix | 20.8 x 20.8, diag. 29.4 | 3200 x 3200 | 6.5 | 10.2 MP | Tested, use at your own risk | v4-5 | |
Teledyne Photometrix Prime BSI | 13.312 x 13.312, diag. 18.8 | 2048 x 2048 | 6.5 | 4.2 MP | Tested, use at your own risk | v4-5 | |
Teledyne Photometrix Iris 15 | 21.49 x 12.61, diag. 24.9 | 5056 x 2960 | 4.25 | 15 MP | Supported and recommended | High resolution across entire FOV only with large-FOV detection objectives. | mesoSPIM-2.0 (Benchtop) |
Controller model | Status | Notes | mesoSPIM version |
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PI C-884 | Supported and recommended | Serves up to 6 stages, including rotation. | v4-5 |
PI C-663 | Tested, use at your own risk | Serves 1 stage, multiple controllers can be used. | v4-5 |
Galil | deprecated | Up to 6 axes (slots) | Used in early versions. |
ASI Tiger (TG8-BASIC) | Supported and recommended | Serves 4 motors with 5 additional free slots. | mesoSPIM2.0 Benchtop |
Model | Max. filter diameter, mm | N(filter slots) | Status | Notes | mesoSPIM version |
---|---|---|---|---|---|
Ludl 96A350 | 32 mm | 10 | Supported and recommended | Large filter wheel with separate controller (MAC6000), serial cable, and high cost. | v4-5 |
Sutter Lambda 10 | 25 | 10 | deprecated | Used in early versions. | |
ZWO EFW-MINI | 31 mm | 5 | Supported and recommended | Very compact and low-cost astronomy filter wheel, with integrated controller and USB cable. | mesoSPIM2.0 Benchtop |
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants