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mdibl_partslist
Fabian Voigt edited this page Aug 28, 2023
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These parts are intended in addition to the mesoSPIM V5 parts. If the setup should be built in this configuration right away, the Hamamatsu Orca camera of the mesoSPIM V5 is not necessary.
The parts list is preliminary - total cost of non-custom parts is approx. $3.5k
Quantity | Component | Comment |
---|---|---|
1 | Custom Aluminium Parts | |
1 | ZWO Filter wheel 7x2" | |
1 | LC6W | 60 mm Cube |
2 | BBE2-E02 2" Elliptical Mirror | 1st & 2nd fold mirror (DM1 & DM2) |
1 | Tube lens Mount (Edmund 11-149) | Tube lens |
1 | Olympus Wide field tube lens | Tube lens |
1 | PI/MICOS L-611 Rotation Stage | Turret stage |
1 | PI/MICOS C-863 Stage Controller with cables | Turret stage |
1 | L-509.2DG (L-509.033111) for the final version or M-111.2DG Linear Stage with cables for the cuvette (prototype) | Turret stage |
1 | KCB2EC/M 45 deg mirror mount | for the 2nd fold mirror |
1 | XT95B-250 Rail | For the Kinetix holder |
1 | XT95P13/M Clamp | For the Kinetix holder |
1 | XT95P13/M Clamp | For the Kinetix holder |
1 | Newport M-CXL95-120 Carrier | For the Kinetix holder |
1 | Newport M-CXL95-80 Carrier | For the Rotation Stage -> check |
2 | Newport M-CXL95-50 Carrier | Stoppers |
1 | VM05/1M Kinematic Mount | for the 1st fold mirror |
1 | 12.7 mm window, 3 mm thick | for the 1st fold mirror |
1 | SM2NFM2 F-Mount Adapter | to connect the Kinetix |
2 | SM2A24 SM2 -> M42x1 Adapter | for the 1.1x & 4x objectives |
1 | M2M32S SM2 -> M32x0.75 Adapt. | for the 12x objective |
1 | RS2P/M 50 mm Post plus spacer | 1st fold mirror mount |
1 | SM2L03 SM2 Tube | to connect the Kinetix |
1 | AM45C/M 45 deg mounting block | 1st fold mirror mount |
1 | SM2CP2 Cap | for the empty position in the 60 mm cube |
1 | SM2L10 1 Inch SM2 Tube | for the 12x objective |
1 | TMA4 | to connect tube lens to 2nd fold mirror |
1 | SM2T2 | to connect tube lens to 2nd fold mirror |
1 | SM2V05 | for the 12x objective |
1 | UBP3/M | to mount the Kinetix |
1 | UBP2/M | to mount the cuvette |
8 | P30/M 1.5 inch posts | to raise excitation paths |
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants