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Nikita Vladimirov edited this page Sep 13, 2023
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Welcome to the mesoSPIM wiki! This wiki serves as documentation on how to set up, align and operate a mesoSPIM.
The mesoSPIM (mesoscale selective plane illumination microscopy) project aims at creating open-hardware microscopy platforms for imaging in cleared tissue. Core features of the current generation mesoSPIM setups are:
- compatibilty with all major clearing techniques including CLARITY, CUBIC, and iDISCO
- large field-of-views, ranging from 2 to 20 mm using an Olympus MVX-10 macroscope
- dual-sided excitation
- optimized for fast screening of whole mouse brain samples, yielding relatively small datasets (10-14 GB/brain/color)
- near-isotropic sampling (6 µm in X,Y & Z) due to an axially swept lightsheet which leads to uniform z-resolution across the field-of-view
- modular sample holders and quick sample exchange
- large travel range (45 x 45 x 100 mm) to allow imaging of very large samples
Inspired by the openSPIM and openSPIN projects, the documentation for building and extending your own mainframe mesoSPIM is available here.
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants