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mdibl_lavision_test
Fabian Voigt edited this page Aug 28, 2023
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These are test data acquired from a Thy1-mouse brain imaged in DBE using the Olympus SWTLU-C tube lens and an Hamamatsu Orca Flash 4.0 V2 camera. Note that using the Kinetix camera, the FOV is a factor of 1.56 larger. Especially the 4x and 12x Objectives have excellent image quality.
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants