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Sample storage
Nikita Vladimirov edited this page Jul 7, 2021
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Samples that are used frequently can be stored in immersion cuvettes. The cuvettes can be mounted on a custom-made rack, laser-cut or 3D printed from black plastic, and fitted into a storage box. Design files for the cuvette rack: Inventor .ipt, drawing .pdf.
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants