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mesoSPIM_preparing_imaging_pc
Fabian F. Voigt edited this page Mar 16, 2019
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- Install the framegrabber supplied with the Hamamatsu Orca camera (Active Silicon).
- Install the MXI-Bus card for the NI-PCIe extension box / NI chassis containing the NI AO cards.
- Install a PCIe to RS-232 card for the filter wheel connection.
⚠️ The MAC-6000 filterwheel controller needs a null-modem RS-232 cable. Use the cable supplied with the MAC-6000.
- Install the DCAM-API / Active Silicon drivers for the Hamamatsu camera. Test the camera using HCimage.
- Plug the USB to RS-485 converter for the zoom servo into the PC. Usually, drivers should be installed automatically.
- Install the NI-DAQmx drivers and NI-Max (Labview is not necessary). Test the analog outputs with the test panels in NI-Max.
- Install the Optotune software to set the initial ETL voltage-to-current conversion table as described here. The download link is available after registration at optotune.com
- Install the Physik Instrumente software as described on the wiki page covering the stage installation.
- Follow the instructions for installing the mesoSPIM software on the mesoSPIM-control repository
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants