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mesoSPIM_excitation_path_L1_collimation
Fabian F. Voigt edited this page Mar 11, 2019
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Use another CPVM plate to set up the laser collimator. Add two 4-inch cage rods to the bottom, and screw in the 75 mm collimation lens on the bottom side.
Add the laser shutter with its cageplate and a standard SM1 cage plate with the SM1FCA fiber adapter. Fixate the SM1FCA and the shutter inside their respective cageplates so that their distance is minimal.
A SM1L10 tube and a SM1V10 adjustable lens tube can be used to move the entire stack of the two cage plates axially for collimation under control of a shearing interferometer.
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants