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Useful primers we have in the lab
Anum Azam Glasgow edited this page Mar 31, 2023
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100 µM stocks are in the -20 °C freezer. Dilute to 5 µM and send 5 µl per Sanger sequencing reaction.
Name | Sequence | Description |
---|---|---|
AAG262F | GTTGGAACCTCTTACGTGCC | pAAG16 FWD sequencing primer |
AAG263R | ggtgcctcactgattaag | AMP REV sequencing primer |
AAG266F | CGATCCAGCCTCCGGAC | pcDNA FWD sequencing primer |
AAG267R | TGTAATCCAGAGGTTGATTGTCGA | pcDNA REV sequencing primer |
AAG271F | AAACCAGTAACGTTATACGATGTCGCA | LacI FWD sequencing primer |
AAG272R | CTGCCCGCTTTCCAGTCG | LacI REV sequencing primer |
AAG273F | cgaaattaatacgactcactatag | T7 FWD sequencing primer |
AAG274F | TCCCTATCAGTGATAGAGATTG | pTET FWD sequencing primer |
AAG275F | CAATTGTGAGCGGATAACAA | p_LlacO-1 sequencing primer |
AAG290R | CCCGACCTCGACAAGG | pCMV FWD sequencing primer |
AAG291F | tgaagctccggttttgaact | pUBQ FWD sequencing primer |
AAG293R | CCTTGTCGAGGTCGGG | pCMV REV sequencing primer |
AAG294R | gctagttattgctcagcgg | T7 terminator REV primer |
AAG295R | ccttctgataggcagcct | pUBQ REV sequencing primer |
AAG302F | GAGAATCTGTACTTTCA | TEV fwd primer |
AAG303R | GGCCTGAACGACATCTT | AVI TAG fwd primer |
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