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Useful primers we have in the lab

Anum Azam Glasgow edited this page Mar 31, 2023 · 8 revisions

100 µM stocks are in the -20 °C freezer. Dilute to 5 µM and send 5 µl per Sanger sequencing reaction.

Name Sequence Description
AAG262F GTTGGAACCTCTTACGTGCC pAAG16 FWD sequencing primer
AAG263R ggtgcctcactgattaag AMP REV sequencing primer
AAG266F CGATCCAGCCTCCGGAC pcDNA FWD sequencing primer
AAG267R TGTAATCCAGAGGTTGATTGTCGA pcDNA REV sequencing primer
AAG271F AAACCAGTAACGTTATACGATGTCGCA LacI FWD sequencing primer
AAG272R CTGCCCGCTTTCCAGTCG LacI REV sequencing primer
AAG273F cgaaattaatacgactcactatag T7 FWD sequencing primer
AAG274F TCCCTATCAGTGATAGAGATTG pTET FWD sequencing primer
AAG275F CAATTGTGAGCGGATAACAA p_LlacO-1 sequencing primer
AAG290R CCCGACCTCGACAAGG pCMV FWD sequencing primer
AAG291F tgaagctccggttttgaact pUBQ FWD sequencing primer
AAG293R CCTTGTCGAGGTCGGG pCMV REV sequencing primer
AAG294R gctagttattgctcagcgg T7 terminator REV primer
AAG295R ccttctgataggcagcct pUBQ REV sequencing primer
AAG302F GAGAATCTGTACTTTCA TEV fwd primer
AAG303R GGCCTGAACGACATCTT AVI TAG fwd primer
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