Colony PCR

1. Place 50 ul of ddH2O in a 1.5 ml tube.
2. Scrape a decent-sized colony from a plate and dissolve in the water.
3. Heat at 95-98 °C for 5-10 min.
4. Centrifuge at 12000 x g for 1 min.
5. Use 10 ul of the supernatant as a template for the following PCR:

Ingredient	Volume
template	5 ul
oligo 1	1.25 ul
oligo 2	1.25 ul
Q5 2X MM	12.5 ul
water	5 ul