Calibrate MS

Good to calibrate the MS before each run, although PIGEON can salvage a run that was miscalibrated >7ppm

• run binary pump at 90%B for >10min
• pour ~0.5ml from agilent low tune mix bottle in deli fridge into epi tube
• suck up the tune mix with the syringe (~0.5ml)
• switch on calibration pump and pump for ~10min to fill capillary
• switch source inlet to calibration pump
• set to MS only mode
• can keep other parameters (dry temp, spectra rate, etc.) the same
• once you can see big peaks (such as 922da), go to Calibration tab and then hit calibrate a few times until all the errors are low (<1)
• hit accept
• method->save
• switch source inlet back to leap, shutoff calibration pump
• run binary pump for 90%B for >10min again before changing to desired %B