Analyze intact mass on Compass

• in compass, on your TIC/BPC, right click and drag to select small range
• This will display in spectrum view. Look for an envelope. Confirm that noise is low-ish
• right click copy to compound spectra
• you can use charge state rule (on top toolbar) to check that you're looking at the right protein (approximate mass)
• Deconvolute->parameters
• -max entropy mode
• -change masses to narrow range around your protein (+-1kD)
• -Instrument resolving power: 50,000
• -resolution: High
• -Apply, OK
• Deconvolute->Mass spectra
• Wait for green bar
• expand the compound entry to reveal deconvoluted entry below
• zoom out of compound spectra to see intact mass envelope
• celebrate a job well done