In‐vitro

Excel templates for in-vitro assays

Each folder consists of Excel and JSON file for the specific assay.

Barrier crossing

• Barrier integrity - LY INVITRO_BARRIERCROSSING_barrier integrity - LY_COLUMNS.xlsx
• NP crossing ICP-OES INVITRO_BARRIERCROSSING_NP crossing ICP-OES_COLUMNS.xlsx
• NP crossing ToF-SIMS INVITRO_BARRIERCROSSING_NP crossing ToF-SIMS_COLUMNS.xlsx
• NP intracell pen(confocal micro [INVITRO_BARRIERCROSSING_NP intracell pen(confocal micro_COLUMNS.xlsx] (https://github.com/enanomapper/nmdataparser/tree/master/enmconvertor/src/site/resources/templates/INVITRO/BARRIERCROSSING)
• NPs crossing TEM-SEM INVITRO_BARRIERCROSSING_NPs crossing TEM-SEM_COLUMNS.xlsx
• Oral mucosa 3Dmodel penetration INVITRO_BARRIERCROSSING_oral mucosa 3Dmodel penetration_COLUMNS.xlsx
• Transepithelial electrical resistance (TEER) INVITRO_BARRIERCROSSING_TEER_COLUMNS.xlsx
• TEM observation
• TEM-SEM - intracell penetration INVITRO_BARRIERCROSSING_TEM-SEM - intracell penetration_COLUMNS.xlsx

Cell Transformation Assay

• hCTA INVITRO_CTA_hCTA_COLUMNS

Cytotoxicity

• Lactate Dehydrogenase (LDH) Assay INVITRO_CYTOTOXICITY_LDH_COLUMNS.xlsx
• MTS INVITRO_CYTOTOXICITY_MTS_COLUMNS.xlsx
• Resazurin INVITRO_CYTOTOXICITY_Resazurin_COLUMNS.xlsx
• Trypan blue INVITRO_CYTOTOXICITY_Trypan blue_COLUMNS.xlsx

Genotoxicity

• Chromosomal damage in vitro INVITRO_GENOTOXICITY_chromosomal damage in vitro_COLUMNS.xlsx
• COMET INVITRO_GENOTOXICITY_COMET_COLUMNS.xlsx
• Micronucleus INVITRO_GENOTOXICITY_Micronucleus_COLUMNS.xlsx
• TEM observation

Immunotoxicity

• Apoptosis INVITRO_IMMUNOTOX_Apoptosis_COLUMNS.xlsx
• Cytokine secretion INVITRO_IMMUNOTOX_Cytokine secretion_COLUMNS.xlsx
• NO production INVITRO_IMMUNOTOX_NO production_COLUMNS.xlsx

Nanomaterial deposition

• Aerosol Characterisation INVITRO_NMDEPOSITION_Aerosol Characterisation_COLUMNS.xlsx
• Aspect of deposit INVITRO_NMDEPOSITION_Aspect of deposit_COLUMNS.xlsx
• TEM observation

Occular irritation

• hemolysis INVITRO_OCCULARIRRITATON_hemolysis_COLUMNS

Cell viability

• Alamar blue INVITRO_VIABILITY_Alamar blue_COLUMNS.xlsx
• Colony Forming Efficiency (CFE) assay INVITRO_VIABILITY_CFE_COLUMNS.xlsx
• Impedance flow cytometry INVITRO_VIABILITY_Impedance_flow cytom_COLUMNS.xlsx
• LDH INVITRO_VIABILITY_LDH_COLUMNS.xlsx
• MTS INVITRO_VIABILITY_MTS_COLUMNS.xlsx
• Neutral red INVITRO_VIABILITY_Neutral red_COLUMNS.xlsx
• Resazurin INVITRO_VIABILITY_Resazurin_COLUMNS.xlsx
• RT- Impedance adherence cell INVITRO_VIABILITY_RT- Impadence_adherence cell_COLUMNS.xlsx
• Trypan blue INVITRO_VIABILITY_Trypan blue_COLUMNS.xlsx