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pdimens committed Aug 1, 2024
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Expand Up @@ -38,11 +38,11 @@ It's recommended to have at least 10X-12X depth to get decent structural variant
(definitely read that in a paper that I would like to link here, but I can't seem to find
it). If your data already has a minimum of 10X for each individual, great! Feel free to use
variant callers like `naibr` and `leviathan` to identify structural variants. However, if
you opted to sequence more individuals at lower coverage (lcWGS is often between 0.5-5X),
you opted to sequence more individuals at lower depth (lcWGS is often between 0.5-5X),
then calling structural variants in individuals may be a challenge.

## The solution
One way to get your low-coverage data and still call structural variants is to pool
One way to get your low-coverage (low depth) data and still call structural variants is to pool
samples together, which would effectively boost the depth. By doing this, you will
no longer be able to make per-individual assessments, which can be fine depending on
the nature of your study.
Expand All @@ -54,9 +54,9 @@ haplotag data is just whole genome sequence data plus a little extra information
use the SNPs of your data to first identify genetic clusters as per standard population
genetic practices. Once population structure/stratification has been identified, you can use
that as a basis to pool together samples from these groups. As an example, you can use
[pcangsd](https://github.com/Rosemeis/pcangsd) to perform a PCA on low-coverage SNP data and
[pcangsd](https://github.com/Rosemeis/pcangsd) to perform a PCA on low-depth SNP data and
get results like these:
![PCA of Alosa sapidissima (SNPs from low-coverage haplotag dataset)](/static/pca.png)
![PCA of Alosa sapidissima (SNPs from low-depth haplotag dataset)](/static/pca.png)

Given these results, a sensible pooling strategy may be:
- **Pool 1**: Miramichi samples
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