Error in if (tolower(filter) == "novar") { : missing value where TRUE/FALSE needed In addition: Warning messages: 1: In asMethod(object) : sparse->dense coercion: allocating vector of size 21.9 GiB 2: In readSignaturesInput(signatures) : NAs introduced by coercion

[ltgmri]

when I run the below codes:

Sys.setenv(LANG = 'EN')
load("scRNA1_ann.Rdata")

library(scMetabolism)
library(ggplot2)
library(rsvd)
library(Seurat)

DefaultAssay(scRNA1) <- "RNA"

countexp.Seurat<-sc.metabolism.Seurat(obj = scRNA1, method = "VISION", imputation = F, ncores =65, metabolism.type = "KEGG")

I encounter an error：

Error in if (tolower(filter) == "novar") { :
missing value where TRUE/FALSE needed
In addition: Warning messages:
1: In asMethod(object) :
sparse->dense coercion: allocating vector of size 21.9 GiB
2: In readSignaturesInput(signatures) : NAs introduced by coercion

Is there anyone encounter the same error, and how to solve it? thank you?

[baiyaer112]

I got the problem too，but i fix it by devtools::install_github("YosefLab/[email protected]")，download error can fix it by options(timeout = 600000000) then run comment devtools::install_github("YosefLab/[email protected]") agint