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update readme
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wbaopaul committed Oct 29, 2020
1 parent bf57637 commit 41ef496
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8 changes: 4 additions & 4 deletions README.md
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Expand Up @@ -422,12 +422,12 @@ Run scATAC-pro through docker or singularity
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In case you have problem in installing dependencies, you can run scATAC-pro without installing dependencies in **one of** the following ways:

1. Run the pre-built dockerized version, pull the docker image [here](https://hub.docker.com/r/wbaopaul/scatac-pro)
1. Run the pre-built dockerized version, pull the docker image [here](https://hub.docker.com/r/wbaopaul/scatac-pro)

2. Run it through singularity (which is more friendly with high performance cluster or HPC, and linux server) by running the following command:

```
$ singularity pull -F docker://wbaopaul/scatac-pro
$ singularity pull -F docker://wbaopaul/scatac-pro:latest
## will generate scatac-pro_latest.sif in current directory
$ singularity exec -H YOUR_WORK_DIR --cleanenv scatac-pro_latest.sif scATAC-pro -s XXX -i XXX -c XXX
Expand All @@ -441,11 +441,11 @@ $ singularity exec -H YOUR_WORK_DIR --cleanenv scatac-pro_latest.sif scATAC-pro
#!/bin/bash
module load singularity
singularity pull -F docker://wbaopaul/scatac-pro ## you just need run this line once
singularity pull -F docker://wbaopaul/scatac-pro:latest ## you just need run this line once
## will generate scatac-pro_latest.sif in the current directory
singularity exec --cleanenv -H /mnt/isilon/tan_lab/yuw1/run_scATAC-pro/PBMC10k scatac-pro_latest.sif \
scATAC-pro -s mapping -i fastq_file1,fastq_file2 -c configure_user.txt
scATAC-pro -s mapping -i fastq_PE1_file,fastq_PE2_file -c configure_user.txt
# and then qsub mapping.sh
```
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2 changes: 1 addition & 1 deletion complete_update_history.md
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@@ -1,6 +1,6 @@
## Complete Update History
- Current version: 1.2.0
* update footprint dependent rgt-hint module to python3
* update footprint dependency *rgt-hint* module to python3
* save qc statistics in html report into tables, and peak calling summary inf added in the report
* add qc per cell to seurat obj metadata as: total.unique.frags, frac.peak, frac.mito,
frac.tss, frac.promoter, and frac.enhancer
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