Update duplicate_alignment_spotfeature.qmd

duplicate_alignment_spotfeature.qmd

@@ -17,20 +17,20 @@ This tutorial demonstrates how to separate isomeric compounds in lipidomics and
 
 Next, click on "Show Ion Table" to view the lipids annotated by MS-DIAL. Further, proceed with the peak picking process. If you identify isomeric compounds within your data, follow the subsequent steps to separate those isomeric mixtures effectively (Figure 1). 
 
-![Figure 1: Start up a project in MS-DIAL 5.3. First, select your processed lipidomics or metabolomics project to load it into the software (A). Next, select “Show Ion Table" to view the list of lipids annotated by MS-DIAL (B). Do the peak picking and identify isomeric compounds in your data (C). Check the aligned spot and isomeric peaks in the EIC of aligned spot](images/Duplicate alignment spot features/Figure_1 for website.png){#fig-1}
+![Start up a project in MS-DIAL 5.3. by selecting your processed lipidomics or metabolomics project (A). Once the project is loaded, click on the "Show Ion Table" to view the list of lipids annotated by MS-DIAL (B). Proceed with peak picking and the identification of isomeric compounds in the data (C). Examine the aligned spots and isomeric peaks by checking in the EIC of aligned spot (D).](images/Duplicate alignment spot features/Figure_1 for website.png){#fig-1}
 
 Select the lipid that appears as isomers in the ion table. Next, navigate to the EIC (Extracted Ion Chromatogram) of the aligned spot to view the peaks, and use the alignment spot viewer to examine the isomeric compounds. To duplicate the isomeric peaks, right-click and drag to zoom in on the alignment spot of the isomeric compounds within the alignment spot viewer. Then, right-click on the aligned spot and select "Duplicate Selected Peak Spot." Finally, return to the ion table, where the duplicated peak of the isomeric compound, will appear at the bottom.
 
 Select the duplicated isomeric peak and recheck the peaks in the EIC of the aligned spot. Minimize the ion table, then right-click on the EIC of the aligned spot and select "Peak Curation (EICs Overlay)" to begin separating the isomers. The "Aligned Chromatogram Modification" window will appear, displaying the original EIC, aligned chromatograms, and manually modified chromatograms. Right-click on the aligned chromatograms and drag to select the low-intensity peak of the duplicated isomer, and click "Update." After updating, close the "Aligned Chromatogram Modification" window, reopen the ion table, and refresh the list. The separated isomeric peak of the duplicated isomer can now be seen in the EIC of the aligned spot. For better clarity, consider renaming the duplicate peak to facilitate understanding (Figure 2 and 3). 
 
-![Figure 2: Once the isomeric peak is selected, click the duplicate selected peak spot option for duplicating the isomer (A). Check the duplicate isomeric peak at the bottom of the ion table (B). Next, select the peak curation (EIC overlays) to adjust the intensity of the peak (C). Select the peak intensity for the duplicate isomeric peak in the aligned chromatograms (D)](images/Duplicate alignment spot features/Figure_2 for website.png){#fig-2}
+![After selecting the isomeric peak, click on the "Duplicate Selected Peak Spot" option to create a duplicate for the isomer (A). Verify the duplicated isomeric peak will appear at the bottom of the ion table (B). Select the peak curation (EIC overlays) to adjust the intensity of the peak (C). Finally, adjust the peak intensity for the duplicated isomeric peak in the aligned chromatogram (D)](images/Duplicate alignment spot features/Figure_2 for website.png){#fig-2}
 
 Next, open the ion table and select the original isomeric peak. Follow a similar process to separate this peak by rechecking the peaks in the EIC of the aligned spot. Right-click on the EIC of the aligned spot and choose the "Peak Curation (EICs Overlay)" option to begin the separation process. In the "Aligned Chromatogram Modification" window, right-click and drag to select the high-intensity peak of the original isomeric peak from the aligned chromatograms, then click "Update" to apply the changes. After updating, refresh the ion table to see the separated peak intensity of the original isomeric peak, distinct from the duplicated isomer (Figure 3). 
 
-![Figure 3: Refresh the ion table and rename the duplicate isomeric peak for better understanding (A). Next, click on the original isomeric peak and cross-verify the peaks in the EIC of aligned spot (B). Select the peak curation (EIC overlays) to adjust the intensity of the original peak (C). Select the peak intensity for the original isomeric peak in the aligned chromatograms and click update (D)](images/Duplicate alignment spot features/Figure_3 for website.png){#fig-3}
+![Refresh the ion table and rename the duplicated isomeric peak for better understanding (A). Next, select the original isomeric peak and cross-verify the peaks in the EIC of aligned spot (B). Click on peak curation (EIC overlays) to adjust the intensity of the original peak (C). Finally, select the peak intensity for the original isomeric peak in the aligned chromatogram and click "Update" to confirm the changes (D)](images/Duplicate alignment spot features/Figure_3 for website.png){#fig-3}
 
 
 To get a clearer view, right-click and drag to zoom in on the alignment spot of the separated isomeric peak within the Alignment Spot Viewer. Finally, save your project and export the results (Figure 4).
 
-![Figure 4: Refresh the ion table and cross-verify the separation of the isomeric peaks by clicking the original isomeric peak (A) and alignment spot (B).](images/Duplicate alignment spot features/Figure_4 for website.png){#fig-4}
+![Refresh the ion table and cross-verify the separation of the isomeric peaks by selecting the original isomeric peak (A) and alignment spot (B)](images/Duplicate alignment spot features/Figure_4 for website.png){#fig-4}