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wolflab-scripts

This encompasses 2 projects

  1. Genome Assembly & Downstream Analysis of 192 Bacteriodetes strains
  • generate_adapter_files.py - generate FASTA adapter files for trimmomatic
  • genome_assembly_pipeline.sh - self-descriptive, preprocesses reads, assembles genome with SPades
  • preparing_contigs_db.sh - preprocessing for Anv'io
  • anvio_pangenomic.sh - performing pangenomic analysis in Anv'io
  • add_zero.py - helper script to reformat files
  • run_kraken_all.sh - helper script for running kraken2
  • run_prokka_all.sh - helper script for genome annotation
  1. Quantitifying Presence of Mycobacterium Tuberculosis in 108 Pediatric Fecal Samples
  • kraken2_bowtie.sh - filter raw reads & analyse with kraken2
  • extract_reads.sh - extract reads classified as TB by Kraken2
  • count_k_reads.py - helper script to count reads mapped to mtb by kraken2
  • multiple_blast_queries.sh - BLAST extracted reads
  • parse_blast_output.py - parse blast output from samples

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