v1.1.0 – Shang Tang
[1.1.0] - Shang Tang - [2023-12-20]
Enhancements & fixes
- Updated the CI procedure to use "sanger-tol" rather than "nf-core" names.
- Renamed Sanger related Github CI test workflows.
- nf-core template was updated from 2.7 to 2.8.
- Removed BAM/CRAM index files from the sample sheets.
- Made fasta index file optional from the inputs.
- Imported PacBio readmapping sub-workflows from sanger-tol/readmapping pipeline. Therefore, the pipeline can run on unaligned BAM/CRAM samples now.
- Use VCFtools to calculate per site nucleotide diversity.
- Use VCFtools to calculate heterozygosity.
Parameters
This release with the following initial parameters:
| Old parameter | New parameter |
|---|---|
| --gzi | |
| --vector_db | |
| --align | |
| --include_positions | |
| --exclude_positions |
NB: Parameter has been updated if both old and new parameter information is present.
NB: Parameter has been added if just the new parameter information is present.
NB: Parameter has been removed if new parameter information isn't present.
Software dependencies
Note, since the pipeline is using Nextflow DSL2, each process will be run with its own Biocontainer. This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. Only Docker or Singularity containers are supported, conda is not supported.
| Dependency | Old version | New version |
|---|---|---|
| DeepVariant | 1.4.0 | 1.5.0 |
| samtools | 1.16.1 | 1.17 |
| bcftools | 1.16.1 | 1.17 |
| python | 3.11.0 | 3.11.4 |
| vcftools | 0.1.16 | |
| blast | 2.14.1+ | |
| gunzip: | 1.10 | |
| minimap2 | 2.24-r1122 | |
| awk | 5.1.0 | |
| untar | 1.30 |
NB: Dependency has been updated if both old and new version information is present.
NB: Dependency has been added if just the new version information is present.
NB: Dependency has been removed if version information isn't present.