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jgrg committed Jun 4, 2024
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Expand Up @@ -38,53 +38,24 @@ Genomes on a Tree (GoaT), an Elasticsearch-powered datastore and search index fo

## Oatk

Developed for _de novo_ assembly of complex plant organelle genomes using
PacBio HiFi data, [Oatk](https://github.com/c-zhou/oatk) can also be used to
assemble other, simpler organelle genomes such as animal mitochondria. From
whole genome sequencing data input, Oatk's tools produce annotation
(using profile HMMs from [OatkDB](https://github.com/c-zhou/OatkDB.git)) and
cirularised graph assemblies of plastid and mitochondrial genomes.
Developed for _de novo_ assembly of complex plant organelle genomes using PacBio HiFi data, [Oatk](https://github.com/c-zhou/oatk) can also be used to assemble other, simpler organelle genomes such as animal mitochondria. From whole genome sequencing data input, Oatk's tools produce annotation (using profile HMMs from [OatkDB](https://github.com/c-zhou/OatkDB.git)) and cirularised graph assemblies of plastid and mitochondrial genomes.

<br/>

## Pretext

Pretext is a suite of tools used to curate the chromosome level structure of long read assemblies of novel genomes using Hi-C contact maps:

1. [PretextMap](https://github.com/sanger-tol/PretextMap)
- [PretextMap](https://github.com/sanger-tol/PretextMap) - Produces a Pretext map file from Hi-C data mapped to an assembly in SAM/BAM/CRAM or [Pairs](https://github.com/4dn-dcic/pairix/blob/master/pairs_format_specification.md) format. The Pretext map file stores the Hi-C contact map in an OpenGL texture.

Produces a Pretext map file from Hi-C data mapped to an assembly in
SAM/BAM/CRAM or
[Pairs](https://github.com/4dn-dcic/pairix/blob/master/pairs_format_specification.md)
format. The Pretext map file stores the Hi-C contact map in an OpenGL
texture.
- [PretextGraph](https://github.com/sanger-tol/PretextGraph) - Used to add BED-Graph format anntoation to a Pretext map file.

2. [PretextGraph](https://github.com/sanger-tol/PretextGraph)
- [PretextView](https://github.com/sanger-tol/PretextView) - An interactive, OpenGL based viewer and editor of Pretext map files, used to curate the chromosome level structure of assemblies. The edited genome is exported in an AGP format file, and includes tags introduced during curation which label contaminants, haplotigs, chromosome names and haplotypes.

Used to add BED-Graph format anntoation to a Pretext map file.

3. [PretextView](https://github.com/sanger-tol/PretextView)

An interactive, OpenGL based viewer and editor of Pretext map files, used to
curate the chromosome level structure of assemblies. The edited genome is
exported in an AGP format file, and includes tags introduced during
curation which label contaminants, haplotigs, chromosome names and
haplotypes.

4. [PretextSnapshot](https://github.com/sanger-tol/PretextSnapshot)

Extracts Hi-C contact map images from Pretext map files.
- [PretextSnapshot](https://github.com/sanger-tol/PretextSnapshot) - Extracts Hi-C contact map images from Pretext map files.

<br/>

## AGP / TPF Utilities

Nucleotide coodinates (other than the start or end of contigs) in AGP files
produced by
[PretextView](https://github.com/sanger-tol/PretextView)
are only accurate to the number of bases spanned by each pixel in its
map file. The `pretext-to-tpf` command of
[AGP / TPF Utilites](https://github.com/sanger-tol/agp-tpf-utils/)
uses the orginal, unedited assembly to correct the coordinates of the assembly
contigs. It also uses tags from PretextView to label chromosomes, and to
separate haplotypes, haplotigs and contaminants.
Nucleotide coodinates (other than the start or end of contigs) in AGP files produced by [PretextView](https://github.com/sanger-tol/PretextView) are only accurate to the number of bases spanned by each pixel in its map file. The `pretext-to-tpf` command of [AGP / TPF Utilites](https://github.com/sanger-tol/agp-tpf-utils/) uses the orginal, unedited assembly to correct the coordinates of the assembly contigs. It also uses tags from PretextView to label chromosomes, and to separate haplotypes, haplotigs and contaminants.

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