Skip to content

RCC workshop, "Analysis of Genetic Data 1: inferring population structure."

License

Notifications You must be signed in to change notification settings

rcc-uchicago/genetic-data-analysis-1

Repository files navigation

Analysis of Genetic Data 1: Inferring Population Structure

During this short workshop, we will apply simple numeric techniques such as principal components analysis (PCA) to investigate human genetic diversity and population structure from large-scale genetic data sets. We will investigate how large genetic data sets are commonly represented in computer files, and we will use popular command-line tools such as PLINK to prepare the "raw" genetic data for analysis. We will use R to compute principal components from the genetic data and visualize the results. This workshop is mainly intended to develop practical computing skills for researchers working with genetic data—concepts such as "genotype" and "allele frequency" will not be explained. This will be a hands-on workshop and we will do "live coding" throughout, so please bring your laptop!

Attendees will: (1) work through the steps of a basic population structure analysis in human genetics, starting with the “raw” source data, and ending with a visualization of population structure estimated from the genetic data; (2) understand how large genetic data sets are commonly represented in computer files; (3) use command-line tools (e.g., PLINK) to manipulate the genetic data; (4) use R to compute principal components, and visualize the results of PCA; (5) learn through "live coding."

Prerequisites

This hands-on workshop assumes participants are already familiar with R and a UNIX-like shell environment. An RCC user account is recommended, but not required. Guest access to the RCC cluster will be available in class to those with no RCC account. All participants must bring a laptop with a Mac, Linux, or Windows operating system that they have administrative privileges on.

Notes on data files

  • 1kg.pop describes the population labels used in the 1000 Genomes data. This information comes from Supplementary Table 1 of the most recent 1000 Genomes paper (Nature, 2015, doi:10.1038/nature15393).

  • omni_samples.20141118.panel was downloaded from this FTP location: ftp.1000genomes.ebi.ac.uk/vol1/ftp/release/20130502/supporting/hd_genotype_chip

  • 20140625_related_individuals.txt was downloaded from this FTP location: ftp.1000genomes.ebi.ac.uk/vol1/ftp/release/20130502. This file gives information about the 31 genotype samples that were found to be closely related. The columns in the file from left to right are: (1) sample; (2) population; (3) gender; and (4) reason for exclusion.

Other information

Credits

These materials were developed by Peter Carbonetto at the University of Chicago. Thank you to Matthew Stephens for his support and guidance.

About

RCC workshop, "Analysis of Genetic Data 1: inferring population structure."

Resources

License

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published