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adding config ev example
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aineniamh committed Jan 29, 2024
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Expand Up @@ -441,6 +441,13 @@ Pitfalls to note:

Suggested configuration settings can be found [here](https://github.com/polio-nanopore/piranha/blob/main/docs/config.ev.yaml) and we would recommend to only use this mode if the user can sufficiently appreciate the limitations the reference file composition may have on detection.

With the supplied config file, piranha can be run as follows:

```
piranha -c config.ev.yaml
```


## Experimental haplotype calling pipeline

There is now an experimental haplotype calling pipeline that uses freebayes for initial variant calling and flopp for read phasing with the called variants. It has a number internal QC steps for merging identical haplotypes. This pipeline requires further validation, but can theoretically produce multiple consensus sequences for each poliovirus population present within a sample. The pipeline has mostly be tested on VDPVs of two mixtures, but will be further assessed.
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