Cryopreservation of a soil microbiome using a Stirling Cycle approach - a genomic assessment

Introduction

This project explores the cryopreservation of soil samples, a crucial aspect of advancing microbiome research. We focus on maintaining the integrity of the soil microbiome, which is often stored in refrigerated or frozen states by various institutions. Our study involves the use of a controlled rate cooler to assess the genomic content of a representative soil sample before and after cryopreservation. We also compare two cryopreservation methods.

Table of contents

16S Sequencing data

01-Steps in proessing the 16s (Markdown)

02A-R data analysis (Rmarkdown)

02B-R data analysis (Markdown)

03-qiime2 outcomes

04-figaro outcomes

05-Metadata (TXT)

ITS Sequencing data

01-Steps in proessing the ITS (Markdown)

02A-R data analysis (Rmarkdown)

02B-R data analysis (Markdown)

03-qiime2 outcomes

04-(figaro outcomes

05-Metadata (TXT)

Figures and Supplementary Data

01-Figures

Information

Raw sequencing data deposited on the NCBI Sequence Read Archive (SRA) under the ID PRJEB46478

The preprint is available at agrixiv

The sequence analysis was conducted using the UK Crop Diversity: Bioinformatics HPC Resource.