Boba-seq is a barcoded approach for large-scale overexpression screens. See
https://doi.org/10.1101/2022.10.10.511384
bobaseq.R in this code base defines the R functions to turn counts per barcode into fitness values for each fragment and to identify biologically consistent hits. There is also a function to plot the per-fragment fitness values.
To use long reads to associate barcodes with insert locations, use
https://github.com/OGalOz/Boba-seq
To turn BarSeq reads into counts per barcode, use bin/MultiCodes.pl in