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bleaching
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aafkevandenberg committed Jan 7, 2025
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Expand Up @@ -28,6 +28,7 @@ The binding rate :math:`k_{on}` relates to the dissociation constant as.
For this example, we don't know the protein concentration and can therefore not determine :math:`k_{on}` .
We will determine the binding time and refer to the inverse of the binding time, as the *effective binding rate*, :math:`k'_{on} = k_{on}[P]` .
Further, we assume that the *bleaching time* for the dye is much longer than the binding time, such that we can ignore the effect of bleaching.

Load and plot the kymographs
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