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Create af-v2.1.yaml
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raw\/channel_layout\.tsv  - make optional
raw\/images\/[^\/]+\.(?:xml|nd2|oir|lif|czi|tiff|qptiff)  - add qptiff
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j-uranic authored Nov 14, 2024
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89 changes: 89 additions & 0 deletions src/ingest_validation_tools/directory-schemas/af-v2.1.yaml
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files:
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pattern: extras\/.*
required: True
description: Folder for general lab-specific files related to the dataset. [Exists in all assays]
-
pattern: extras\/microscope_hardware\.json
required: True
description: A file generated by the micro-meta app that contains a description of the hardware components of the microscope. Email HuBMAP Consortium Help Desk <[email protected]> if help is required in generating this document.
is_qa_qc: True
-
pattern: extras\/microscope_settings\.json
required: False
description: A file generated by the micro-meta app that contains a description of the settings that were used to acquire the image data. Email HuBMAP Consortium Help Desk <[email protected]> if help is required in generating this document.
is_qa_qc: True
-
pattern: raw\/.*
required: True
description: Raw data files for the experiment.
-
pattern: raw\/channel_layout\.tsv
required: False
description: Table that includes a dictionary for channel to moiety, which may be a protein given in an OMAP panel or captured in the ASCT+B table.
is_qa_qc: False
-
pattern: raw\/images\/.*
required: True
description: Raw image files. Using this subdirectory allows for harmonization with other imaging assays. [This directory must include at least one raw file.]
-
pattern: raw\/images\/[^\/]+\.(?:xml|nd2|oir|lif|czi|tiff|qptiff)
required: True
description: Raw microscope file for the experiment
is_qa_qc: False
-
pattern: lab_processed\/.*
required: True
description: Experiment files that were processed by the lab generating the data.
-
pattern: lab_processed\/images\/.*
required: True
description: Processed image files
-
pattern: lab_processed\/images\/[^\/]+\.ome\.tiff
required: True
description: OME-TIFF files (multichannel, multi-layered) produced by the microscopy experiment. If compressed, must use loss-less compression algorithm. See the following link for the set of fields that are required in the OME TIFF file XML header. <https://docs.google.com/spreadsheets/d/1YnmdTAA0Z9MKN3OjR3Sca8pz-LNQll91wdQoRPSP6Q4/edit#gid=0>
is_qa_qc: False
example: lab_processed/images/HBM892.MDXS.293.ome.tiff
-
pattern: lab_processed\/images\/[^\/]*ome-tiff\.channels\.csv
required: True
description: This file provides essential documentation pertaining to each channel of the accommpanying OME TIFF. The file should contain one row per OME TIFF channel. The required fields are detailed <https://docs.google.com/spreadsheets/d/1xEJSb0xn5C5fB3k62pj1CyHNybpt4-YtvUs5SUMS44o/edit#gid=0>
is_qa_qc: False
-
pattern: lab_processed\/transformations\/.*
required: False
description: This directory contains transformation matrices that capture how each modality is aligned with the other and can be used to visualize overlays of multimodal data. This is needed to overlay images from the exact same tissue section (e.g., MALDI imaging mass spec, autofluorescence microscopy, MxIF, histological stains). In these cases data type may have different pixel sizes and slightly different orientations (i.e., one may be rotated relative to another).
-
pattern: lab_processed\/transformations\/[^\/]+\.txt
required: False
description: Transformation matrices used to overlay images from the exact same tissue section (e.g., MALDI imaging mass spec, autofluorescence microscopy, MxIF, histological stains).
is_qa_qc: False
-
pattern: qa_qc\/.*
required: True
description: Directory containing QA and/or QC information.
-
pattern: qa_qc\/resolution_report\/.*
required: True
description: Directory containing the results of resolution tests and/or vendor preventative maintenance reports.
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pattern: qa_qc\/resolution_report\/resolution\.txt
required: False
description: This file summarizes the results of resolution tests or vendor reports from preventative maintenance visits.
-
pattern: qa_qc\/resolution_report\/[^\/]+\.pdf
required: False
description: This file is a pdf from a vendor preventative maintenance visit or resolution check tool demonstrating resolution. This file may include illumination test results.
-
pattern: qa_qc\/illumination_report\/.*
required: True
description: Directory containing the results of illumination tests and/or vendor preventative maintenance reports.
-
pattern: qa_qc\/illumination_report\/illumination.txt
required: False
description: This file summarizes the results of illumination tests or vendor reports from preventative maintenance visits.
-
pattern: qa_qc\/illumination_report\/[^\/]+\.pdf
required: False
description: This file is a pdf from a vendor preventative maintenance visit or illumination check tool demonstrating illumination intensity.

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