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Update README.md
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huBioinfo authored Feb 24, 2021
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Expand Up @@ -67,7 +67,7 @@ We have developed the CytoTalk algorithm for *de novo* construction of a signali

* A **“BetaUpperLimit.txt”** file indicating the upper limit of the test values of the algorithm parameter β, which is inversely proportional to the total number of genes in a given cell-type pair after removing lowly-expressed genes in the processing of scRNA-Seq data. Based on preliminary tests, the upper limit of β value is suggested to be 100 (default) if the total number of genes in a given cell-type pair is above 10,000. However, if the total number of genes is below 5000, it is necessary to increase the upper limit of β value to 500.

Note that all example input files are in the **/CytoTalk_package_v2.0/Input/** folder and should be customized and copied into the **/CytoTalk_package_v2.0/CytoTalk/** folder before running. The /CytoTalk/ folder can only be used **ONCE** for a given cell-type pair. Please use a new /CytoTalk/ folder for analysis of other cell-type pairs.
Please download **"CytoTalk_package_v2.0.zip"**. All example input files are in the **/Input/** folder and should be customized and copied into the **/CytoTalk/** folder before running. The /CytoTalk/ folder can only be used **ONCE** for a given cell-type pair. Please use a new /CytoTalk/ folder for analysis of other cell-type pairs.

## Run CytoTalk

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