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ErogluLabRotation

Hailey Napier Duke University 10/22/22 - 12/16/22

This repository will serve as documentation for the computational aspects of my rotation project in the Eroglu Lab (10/22/22 - 12/16/22).

See my labarchives folder ("Hailey N. Oct 2022" in the "Rotations Students" directory within Kristina Sakers's labarchives notebook) for full descriptions of experiments and analysis.

Directory and File Descriptions

  • r_scripts contains only r scripts
    • chromhmm_scripts contains r scripts used to analyze and create plots for chromhmm data and data related to chromatin states/chd8 binding
      • chd8_overlap_wt_ko.R is a script to find overlap between chd8 binding sites and chromatin states
      • get_chd8_binding_genes.R is a script to find the genes chd8 binds and their associated GO terms
      • make_bam_binarization_files.R is a script to format the files for binarization for chromhmm
    • image_analysis contains r scripts used to quantify image analysis and make plots for image analysis data
      • 20221206_Cx43_image_analysis.R is a script to analyze connexin43 puncta
  • shell_scripts contains shell scripts I ran on DCC
    • chd8_peaks_intersect_KO_WT.sh is a script to find the overlap of chd8 binding with chromatin states
    • nlgn2_chromhmm.sh is a script to find chromatin states using mintchip .bed files from nlgn2 KO astrocytes
  • backup_scripts.sh is a script to backup DCC shell files on my local computer (to be run on local terminal, not DCC)

Contact [email protected] with questions.

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