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guandailu authored Feb 27, 2022
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## Transcriptome annotation using nanopore sequencing
## Full-length transcript prediction using nanopore sequencing

Pipeline for transcriptome annotation of genomes using nanopore sequencing technology

This project is in process, and documentation and description are still improving.
Pipeline for transcript prediction of the chicken genome using nanopore sequencing technology

## Data preparation
To run this pipeline, following files should be prepared:
1. Reference genome:
* fasta: Gallus_gallus.GRCg6a.dna_sm.toplevel.fa
* gtf: Gallus_gallus.GRCg6a.103.gtf
* gtf: Gallus_gallus.GRCg6a.102.gtf
2. Fastq files:
* tissue_sampleid.fq.gz

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## Running the pipeline
sbatch nanopore_ann_run.sh

N.B. The pipeline is currently configured on UC DAVIS FARM server, which will be updated in the future.

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