PTU_Writer

FIJI plugin generating PicoQuant ptu FLIM TTTR image file given a stack with lifetimes.
Reverse-engineering of PTU_Reader plugin.

How to install plugin

1. You need to download and install FIJI on your computer first.
2. Download PTU_Writer_...jar and place it in the "plugins" folder of FIJI.
3. Plugin will appear as PTU_Writer in FIJI's Plugins menu.

How to run plugin

1. Click PTU_Writer line in ImageJ's Plugins menu.
2. In the following dialog select a TIF Z-stack, where Z corresponds to the "lifetime counts". Only 8- and 16-bit single channel stacks are supported for now.
3. Plugin will ask for "time resolution" metadata parameters:
   
   where TTResult_SyncRate is the laser repetition rate (in Hz) and
   MeasDesc_Resolution is the time between "lifetime counts", i.e. time between two neighboring input z-slices (in ps).
4. In a new (last) dialog choose the folder and name for the output PTU file.

Updates history

v.0.0.2 (2024.11) Added extra tags, now can be loaded via readPTU_FLIM.
v.0.0.1 (2024.11) First release.

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Developed in Cell Biology group of Utrecht University.
Email katpyxa @ gmail.com for any questions/comments/suggestions.