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Auto-Save Index Files Alongside BAMs #446
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Commits on Jan 14, 2023
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Terra changed where submissions go (now at gs://<bucket>/submissions/)
Some small optimizations too.
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Revert those changes because the amount of disk space we need is actu…
…ally too large to benefit from Steve's ComputeAllowedLocalSSD optimization.
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Try to resolve file corruption issue by separating the joint calling …
…and conversion to .g.vcf.bgz steps
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Many updates for short read Malaria processing.
- Added SRFlowcell.wdl, SRWholeGenome.wdl - Added SRJointGenotyping.wdl, LRJointCallGVCFsWithGenomicsDB.wdl - Updated `sr-utils` docker image - Workflow to index a BAM file - Whole genome processing pipeline for short read data - Added short read whole genome pipeline to Dockstore - Added start to workflows to do GCNV calling. - Added LRJointCallGVCFsWithGenomicsDB to dockstore. - Removed downstream calls to `nanoplot` in SRWholeGenome - Added hail matrix table output to LRJointCallGVCFsWithGenomicsDB
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Merge branch 'jts_kvg_sp_malaria' of github.com:broadinstitute/long-r…
…ead-pipelines into jts_kvg_sp_malaria
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Commits on Feb 8, 2023
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Minor updates to variant / joint calling.
- Joint calling now is no longer preemptible. - added `--annotate-with-num-discovered-alleles` flag to HaplotypeCaller.
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Commits on Aug 18, 2023
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Updated CNN model training and haplotypecaller parameters.
- Exposed CNN model training parameters in the WDLs. - Set `HaplotypeCaller` to use the AVX accelerated Smith-Waterman library if it is possible on the executing machine.
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Commits on Sep 5, 2023
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- Added some NaN checking in TrainCnnFilters.wdl - Updated SRWholeGenome and HaplotypeCaller to include an option to use pileup mode.
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Commits on Sep 8, 2023
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Updates for VETS integration and file outputs.
- Updated workflows to use finalized files as outputs instead of files in the working directories. - Fixed validation error in `LRJointCallGVCFsWithGenomicsDB.wdl`. - Updated `SRJointCallGVCFsWithGenomicsDB.wdl` to use VETS instead of VQSR.
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- Added fingerprinting / barcode task, `ExtractFingerprintAndBarcode`, to `VariantUtils.wdl`. - Added call to `ExtractFingerprintAndBarcode` in `SRWholeGenome.wdl`. - Updated version of sr-utils to latest in `SRUtils::BwaMem2`.
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Updates to functional annotation.
- Now FunctionalAnnotation::FunctionallyAnnotateVariants natively produces a VCF Index. - `SRJointCallGVCFsWithGenomicsDB` and `ONTPfTypeDrugResistanceMarkers` now use the new natively-produced VCF index and do not re-index the file. - `ONTPfTypeDrugResistanceMarkers` can now optionally skip to the `CallDrugResistanceMutations` step if the input file is already functionally annotated.
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Added WDL to extract reads from amplicon regions.
Added `ExtractRegionsFromBam.wdl` Added task: `Utils::GetReadsInBedfileRegions` Minor comments added to `VariantUtils::ExtractFingerprintAndBarcode`
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Updated dockstore; Added
PfalciparumDrugResistanceSummary.wdl
- Added `PfalciparumDrugResistanceSummary.wdl` - Added `PfalciparumDrugResistanceSummary.wdl` and `ExtractRegionsFromBam.wdl` to `.dockstore.yml` - parameter_meta change in ExtractRegionsFromBam.wdl
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- Local disk to SSD - samtools index -@ 2 ~{basename(prefix)}
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