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# =========================================================================== # # PUBLIC DOMAIN NOTICE # National Center for Biotechnology Information # # This software/database is a "United States Government Work" under the # terms of the United States Copyright Act. It was written as part of # the author's official duties as a United States Government employee and # thus cannot be copyrighted. This software/database is freely available # to the public for use. The National Library of Medicine and the U.S. # Government have not placed any restriction on its use or reproduction. # # Although all reasonable efforts have been taken to ensure the accuracy # and reliability of the software and data, the NLM and the U.S. # Government do not and cannot warrant the performance or results that # may be obtained by using this software or data. The NLM and the U.S. # Government disclaim all warranties, express or implied, including # warranties of performance, merchantability or fitness for any particular # purpose. # # Please cite the author in any work or product based on this material. # # =========================================================================== The NCBI SRA ( Sequence Read Archive ) Contact: [email protected] http://www.ncbi.nlm.nih.gov/Traces/sra/?view=toolkit_doc The SRA Toolkit and SDK from NCBI is a collection of tools and libraries for using data in the INSDC Sequence Read Archives. Much of the data submitted these days, for example in BAM, Illumina export.txt, and Complete Genomics formats, contain alignment information. With aligned data, NCBI uses Compression by Reference, which only stores the differences in base pairs between sequence data and the segment it aligns to. The process to restore original data, for example as FastQ, requires fast access to the reference sequences that the original data was aligned to. NCBI recommends that SRA users dedicate local disk space to store references downloaded from the NCBI SRA site. As of Fall 2014, the complete collection of these reference sequences is 66 GB. While it isn't usually necessary to download the entirety of the reference sequences, this should give you an idea of the scale of the storage requirement. By default, the Toolkit will download missing reference sequences on demand and cache them in the user's home directory. The location of this cache is configurable, as is whether the download is automatic or manual. For additional information on using, configuring, and building the toolkit, please visit our web site at: http://www.ncbi.nlm.nih.gov/Traces/sra/?view=toolkit_doc&f=std SRA Toolkit Development Team
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