GPR curation for Miscellaneous, Isolated and Drug metabolism

[JHL-452b]

Current behavior:

31 genes are discovered to be uncorrelated with their corresponding reactions in Miscellaneous, Isolated and Drug metabolism.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000167419	P22079	MAR04201: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000121691 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000167419 encodes a heme-containing oxidoreductase enzyme that catalyzes reactions involving thiocyanate, iodide, and phenolic donors in the presence of hydrogen peroxide (H2O2). These reactions relate to antimicrobial defense mechanisms by producing hypothiocyanous acid (OSCN(-)) and hypoiodite (IO(-)). However, the provided reaction (H2O2 + methanol --> formaldehyde + 2.0 H2O) involves the oxidation of methanol to formaldehyde and does not involve thiocyanate, iodide, phenolic donors, or the physiological activities associated with the enzyme from the gene ENSG00000167419. Therefore, the gene is not relevant to the reaction provided.	No Yes
ENSG00000117592	P30041	MAR04201: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000121691 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000117592 encodes a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides into water and alcohols and also exhibits various phospholipase and acyltransferase activities. However, the reaction H2O2 + methanol --> formaldehyde + 2.0 H2O is not among the catalytic activities listed for this gene, which includes catalysis involving peroxides, phospholipids, and lysophospholipids. Therefore, this gene does not relate to the provided reaction.	No Yes
ENSG00000130508	Q92626	MAR04201: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000121691 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000130508 encodes an enzyme that catalyzes various reactions involving the oxidation of bromide, thiocyanate, and iodide, as well as the formation of sulfilimine cross-links in collagen. The reactions this gene is involved in generally include substrates like hydrogen peroxide, bromide, and specific protein residues such as lysyl and methionyl-collagen. The provided reaction, "H2O2 + methanol --> formaldehyde + 2.0 H2O," does not involve any of the substrates or products associated with the functions and catalytic activities of the gene outlined in the texts, such as bromide, collagen residues, or sulfilimine cross-links. Instead, it involves the oxidation of methanol to formaldehyde, a reaction not described in the gene's known activities. Therefore, there is no relevant connection between the gene and the given reaction.	No Yes
ENSG00000198099	P08319	MAR04202: methanol + NAD+ --> formaldehyde + H+ + NADH	ENSG00000147576 or ENSG00000172955 or ENSG00000180011 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000198099 encodes an enzyme that catalyzes the NAD-dependent oxidation of all-trans-retinol, 9-cis-retinol, and long chain omega-hydroxy fatty acids, as well as the reduction of benzoquinones, but it does not catalyze the oxidation of methanol to formaldehyde. The provided reaction concerns the conversion of methanol and NAD+ to formaldehyde, H+, and NADH, which is not among the activities described for this gene according to the provided annotations and PubMed references.	No Yes
ENSG00000180011	Q8N4Q0	MAR04202: methanol + NAD+ --> formaldehyde + H+ + NADH	ENSG00000147576 or ENSG00000172955 or ENSG00000180011 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000180011 encodes a protein that functions as a 15-oxo-prostaglandin 13-reductase and is involved in reactions that reduce 13,14-dihydro-15-oxo-prostaglandins using NADP(+), producing NADPH. The provided reaction involves the oxidation of methanol to formaldehyde using NAD+, producing NADH, which does not align with the catalytic activities of the gene's enzyme. Therefore, the gene is not relevant to the provided reaction.	No
ENSG00000196616	P00325	MAR04202: methanol + NAD+ --> formaldehyde + H+ + NADH	ENSG00000147576 or ENSG00000172955 or ENSG00000180011 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000196616 catalyzes reactions involving the oxidation of all-trans-retinol and its derivatives, such as all-trans-4-hydroxyretinol and all-trans-4-oxoretinol, using NAD+ as a cofactor. These reactions pertain to retinoid metabolism and involve the conversion of these specific substrates to their corresponding retinal forms. However, the reaction provided in the prompt involves the conversion of methanol to formaldehyde using NAD+. Since the gene ENSG00000196616 does not catalyze or participate in the methanol oxidation reaction, it is not relevant to the provided reaction.	No Yes
ENSG00000115705	P07202	MAR08752: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000115705 is involved in iodination and coupling of hormonogenic tyrosines in thyroglobulin to produce thyroid hormones T3 and T4. Its catalytic activities, including reactions involving hydrogen peroxide and iodide with thyroglobulin, do not match the provided reaction, which is the conversion of hydrogen peroxide and methanol to formaldehyde and water.	No
ENSG00000167419	P22079	MAR08752: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000167419 encodes for an enzyme that catalyzes the conversion of thiocyanate into hypothiocyanous acid and iodide into hypoiodite, both in the presence of hydrogen peroxide, and is involved in antimicrobial defense. The reaction provided involves the conversion of hydrogen peroxide and methanol into formaldehyde and water, which is not related to the gene's described catalytic activities.	No Yes
ENSG00000184254	P47895	MAR02195: 3-methoxy-4-hydroxyphenylacetaldehyde + H2O + NADP+ --> 2.0 H+ + homovanillate + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of specific aldehyde substrates such as all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids. The reactions it catalyzes involve substrates like all-trans-retinal and produce NADH as one of the products. However, the reaction provided involves 3-methoxy-4-hydroxyphenylacetaldehyde, NADP+, and produces homovanillate and NADPH, which are different substrates and cofactors. Therefore, the gene's function does not match the reaction provided.	No
ENSG00000184254	P47895	MAR02197: 3-methoxy-4-hydroxyphenylglycolaldehyde + H2O + NADP+ --> 2.0 H+ + NADPH + vanillylmandelate	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates such as all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids. The provided reaction involves the conversion of 3-methoxy-4-hydroxyphenylglycolaldehyde to vanillylmandelate using NADP+ as a cofactor, producing NADPH. This reaction does not involve the substrates or coenzymes specific to the enzyme encoded by ENSG00000184254, indicating that the gene is not relevant to this reaction.	No
ENSG00000196344	P40394	MAR04202: methanol + NAD+ --> formaldehyde + H+ + NADH	ENSG00000147576 or ENSG00000172955 or ENSG00000180011 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000196344 encodes an enzyme that catalyzes the NAD-dependent oxidation of various substrates including all-trans-retinol, alcohols, and omega-hydroxy fatty acids, as well as reduction reactions involving all-trans-retinal and aldehydes. However, the specific reaction outlined¡ªmethanol + NAD+ --> formaldehyde + H+ + NADH¡ªis not among the substrates or reactions mentioned in the gene's annotated catalytic activities. Therefore, the gene does not have relevance to the provided methanol oxidation reaction.	Yes, methanol is an alcohol
ENSG00000117592	P30041	MAR08752: H2O2 + methanol --> formaldehyde + 2.0 H2O	ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The reaction provided is "H2O2 + methanol --> formaldehyde + 2.0 H2O," which involves the conversion of hydrogen peroxide and methanol to formaldehyde and water. The gene ENSG00000117592 encodes a thiol-specific peroxidase that catalyzes the reduction of hydroperoxides to water and alcohols, and also has phospholipase activity. However, there is no mention in the gene's function or catalytic activities that it catalyzes the conversion of hydrogen peroxide and methanol to formaldehyde and water. Therefore, the gene is not relevant to the provided reaction.	No Yes
ENSG00000184254	P47895	MAR02189: 3,4-dihydroxymandelaldehyde + H2O + NADP+ --> 3,4-dihydroxymandelate + 2.0 H+ + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates such as all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids, with a high specificity for all-trans-retinal. The provided reaction involves 3,4-dihydroxymandelaldehyde, H2O, and NADP+ converting to 3,4-dihydroxymandelate, H+, and NADPH, which does not align with the substrates, products, co-factors, or catalytic actions described for ENSG00000184254. Therefore, the gene is not relevant to the given reaction.	No
ENSG00000117592	P30041	MAR02531: H+ + H2O2 + thiocyanate <=> H2O + hypothiocyanite	ENSG00000005381 or ENSG00000117592	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000117592 encodes a thiol-specific peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols and exhibits phospholipase activity. The catalytic activities described for this gene involve reactions such as the reduction of hydroperoxides and conversion of lysophospholipids. However, the provided reaction (H+ + H2O2 + thiocyanate <=> H2O + hypothiocyanite) involves the conversion of hydrogen peroxide and thiocyanate to water and hypothiocyanite, which does not match any of the catalytic activities or functions attributed to the gene in the text. Therefore, the gene is not related to the specified reaction.	No Yes
ENSG00000168137	Q9C0A6	MAR09464: dextrin + H2O --> dextrin(n-1) + glucose	ENSG00000090402 or ENSG00000114982 or ENSG00000168137 or ENSG00000243056 or ENSG00000257335	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000168137 functions as a chromatin regulator involved in brain development by mediating methylation of histone H3. It specifically catalyzes the trimethylation of 'Lys-36' and monomethylation of 'Lys-9' on histone H3, thereby regulating RNA elongation and neural processes. The provided reaction involves hydrolysis of dextrin to glucose, which is unrelated to the gene's role in histone modification and chromatin regulation.	No
ENSG00000243056	O60516	MAR09464: dextrin + H2O --> dextrin(n-1) + glucose	ENSG00000090402 or ENSG00000114982 or ENSG00000168137 or ENSG00000243056 or ENSG00000257335	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000243056 functions as a repressor of translation initiation by regulating EIF4E activity and inhibiting EIF4E-mediated mRNA nuclear export. This function is related to the regulation of protein synthesis, not the hydrolysis of dextrin as described in the reaction. The reaction, involving the breakdown of dextrin into dextrin(n-1) and glucose, does not involve any activity related to translation initiation or mRNA nuclear export, thus the gene is not relevant to the reaction provided.	No
ENSG00000141560	Q9HA64	MAR09466: 1-deoxy-1-(N6-lysino)-D-fructose + ATP <=> ADP + fructoselysine-3-phosphate + H+	ENSG00000116199 or ENSG00000141560 or ENSG00000162408 or ENSG00000167363 or ENSG00000172456	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000141560 encodes for a protein called ketosamine-3-kinase, which is involved in the phosphorylation of ribuloselysine and psicoselysine on glycated proteins, generating ribuloselysine-3 phosphate and psicoselysine-3 phosphate, respectively. The gene explicitly does not phosphorylate fructoselysine, as indicated in the annotation. The provided reaction involves the conversion of 1-deoxy-1-(N6-lysino)-D-fructose with ATP to fructoselysine-3-phosphate, ADP, and H+, which requires phosphorylation of fructoselysine. Therefore, the gene and the reaction are not related.	No
ENSG00000172456	Q96C11	MAR09466: 1-deoxy-1-(N6-lysino)-D-fructose + ATP <=> ADP + fructoselysine-3-phosphate + H+	ENSG00000116199 or ENSG00000141560 or ENSG00000162408 or ENSG00000167363 or ENSG00000172456	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene (ENSG00000172456) is involved in the phosphorylation of D-ribulose to form D-ribulose 5-phosphate as part of a metabolite repair mechanism or the pentose phosphate pathway. However, the reaction provided involves the phosphorylation of 1-deoxy-1-(N6-lysino)-D-fructose to form fructoselysine-3-phosphate, which is a different substrate and product. Therefore, there is no direct link between the gene function and the provided reaction.	No
ENSG00000116199	O75063	MAR09467: ADP + fructoseglycine-ketone-3-phosphate + H+ <=> ATP + fructoseglycine	ENSG00000116199 or ENSG00000141560 or ENSG00000162408 or ENSG00000167363 or ENSG00000172456	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000116199 encodes a protein responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans. Its catalytic activity involves the phosphorylation of a specific serine-linked xylose moiety within the GAG-protein linkage region using ATP. However, the reaction provided pertains to the conversion of ADP and fructoseglycine-ketone-3-phosphate into ATP and fructoseglycine, which is unrelated to the described function and catalytic activity of the gene. Hence, this gene does not participate in the specified reaction.	No
ENSG00000133048	P36222	MAR09485: chitin + H2O --> chitin(n-1) + N-acetylglucosamine	ENSG00000111261 or ENSG00000133048 or ENSG00000133063 or ENSG00000134216	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: According to the provided annotation, the gene ENSG00000133048 encodes a carbohydrate-binding lectin which has no chitinase activity. This gene plays roles in tissue remodeling, immune responses, and bacterial interactions but does not catalyze the hydrolysis of chitin into N-acetylglucosamine. Therefore, it cannot be linked to the reaction described (chitin + H2O --> chitin(n-1) + N-acetylglucosamine).	No
ENSG00000198408	O60502	MAR09495: CoA + H+ + histone-N6-acetyl-L-lysine <=> acetyl-CoA + histone-L-lysine	(ENSG00000005339 and ENSG00000124151) or ENSG00000083168 or ENSG00000084676 or ENSG00000100393 or ENSG00000103510 or ENSG00000108773 or ENSG00000114166 or ENSG00000125484 or ENSG00000128708 or ENSG00000129873 or ENSG00000134014 or ENSG00000134852 or ENSG00000136504 or ENSG00000156650 or ENSG00000172288 or ENSG00000172352 or ENSG00000172977 or ENSG00000182415 or ENSG00000198408	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000198408 encodes a protein that cleaves GlcNAc from O-glycosylated proteins but does not have histone acetyltransferase activity as per the evidence from multiple studies. The provided reaction involves converting histone-N6-acetyl-L-lysine to acetyl-CoA and histone-L-lysine, which is unrelated to the gene's function of deglycosylation.	No
ENSG00000221988	Q9UMR5	MAR09499: H2O + S-palmitoylprotein --> [protein]-L-cysteine + H+ + palmitate	ENSG00000131238 or ENSG00000221988 or ENSG00000241404	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000221988 encodes a protein that removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA, but it is unable to remove palmitate from peptides or proteins due to structural constraints. The reaction described involves the removal of palmitate from a protein (H2O + S-palmitoylprotein --> [protein]-L-cysteine + H+ + palmitate), which the function of this gene does not support. Therefore, the gene and the reaction are not related.	No
ENSG00000213722	O95865	MAR09513: H2O + NG,NG-dimethyl-L-arginine --> citrulline + dimethylamine	ENSG00000153904 or ENSG00000213722	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000213722 is annotated as a putative hydrolase with an unknown substrate and explicitly does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA). The reaction described involves the hydrolysis of N(G),N(G)-dimethyl-L-arginine to citrulline and dimethylamine, which is not a function of this gene according to the annotation. Therefore, based on its function and catalytic activity, this gene is not related to the reaction provided.	No
ENSG00000165591	Q6GMR7	MAR09534: (indol-3-yl)acetamide + H2O --> H+ + indoleacetate + NH3	ENSG00000165591	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000165591 encodes an enzyme that catalyzes the hydrolysis of various fatty acid amides such as oleamide, anandamide, and other fatty amides to their corresponding fatty acids and ethanolamine. Examples listed involve reactions with substrates like (9Z)-octadecenamide and N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine, producing products like (5Z,8Z,11Z,14Z)-eicosatetraenoate and ethanolamine. In contrast, the provided reaction involves the hydrolysis of (indol-3-yl)acetamide to indoleacetate and ammonia, a substrate and product pair not mentioned in the gene's known catalytic activities. Therefore, the gene's annotated functions do not match the given reaction.	No Yes
ENSG00000150656	Q96KN2	MAR06861: H2O + D-alanyl-D-alanine <=> 2.0 alanine	ENSG00000133313 or ENSG00000150656	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000150656 encodes a protein that catalyzes the hydrolysis of Xaa-His dipeptides, with specific activities towards carnosine, anserine, and other similar dipeptides. The reaction involving H2O and D-alanyl-D-alanine to produce two alanine molecules does not involve any substrates or products related to the catalytic activities of this gene's enzyme, such as beta-alanyl-His, beta-alanyl-3-methyl-His, L-alanyl-His, glycyl-His, or L-homocarnosine. Therefore, the gene is not relevant to this reaction.	No
ENSG00000162408	Q5SY16	MAR09466: 1-deoxy-1-(N6-lysino)-D-fructose + ATP <=> ADP + fructoselysine-3-phosphate + H+	ENSG00000116199 or ENSG00000141560 or ENSG00000162408 or ENSG00000167363 or ENSG00000172456	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000162408 encodes a polynucleotide kinase responsible for phosphorylating 5'-hydroxyl groups on RNA and DNA substrates, and is involved in rRNA processing. The reaction provided involves the conversion of 1-deoxy-1-(N6-lysino)-D-fructose and ATP to ADP, fructoselysine-3-phosphate, and H+, which is not related to the polynucleotide kinase function described for the gene.	No
ENSG00000116199	O75063	MAR09466: 1-deoxy-1-(N6-lysino)-D-fructose + ATP <=> ADP + fructoselysine-3-phosphate + H+	ENSG00000116199 or ENSG00000141560 or ENSG00000162408 or ENSG00000167363 or ENSG00000172456	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000116199 encodes an enzyme responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans. It catalyzes the reaction involving the phosphorylation of 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] using ATP to produce 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-2-O-phosphoxylosyl)-L-seryl-[protein], ADP, and H+. However, the reaction provided in the prompt involves the conversion of 1-deoxy-1-(N6-lysino)-D-fructose and ATP to ADP, fructoselysine-3-phosphate, and H+, which is a different biochemical process unrelated to the function and catalytic activity described for the gene ENSG00000116199. Therefore, the gene and the reaction are not related.	No
ENSG00000103275	P63279	MAR09491: [protein]-L-lysine + ATP + ubiquitin --> [protein]-N-ubiquityllysine + AMP + PPi	(ENSG00000103549 and ENSG00000155827) or (ENSG00000126261 and ENSG00000142230) or (ENSG00000121481 and ENSG00000204227) or ENSG00000002746 or ENSG00000005810 or ENSG00000007944 or ENSG00000009335 or ENSG00000011275 or ENSG00000012963 or ENSG00000013561 or ENSG00000024048 or ENSG00000033178 or ENSG00000034677 or ENSG00000049759 or ENSG00000069869 or ENSG00000070423 or ENSG00000070950 or ENSG00000071794 or ENSG00000072401 or ENSG00000072609 or ENSG00000075975 or ENSG00000077152 or ENSG00000077721 or ENSG00000078140 or ENSG00000078747 or ENSG00000078967 or ENSG00000080802 or ENSG00000082996 or ENSG00000085382 or ENSG00000086758 or ENSG00000089234 or ENSG00000090432 or ENSG00000092098 or ENSG00000092148 or ENSG00000099785 or ENSG00000099804 or ENSG00000100814 or ENSG00000101695 or ENSG00000101752 or ENSG00000101871 or ENSG00000102858 or ENSG00000103266 or ENSG00000103275 or ENSG00000103657 or ENSG00000104343 or ENSG00000104517 or ENSG00000105879 or ENSG00000106459 or ENSG00000107341 or ENSG00000107954 or ENSG00000108106 or ENSG00000108523 or ENSG00000108854 or ENSG00000109332 or ENSG00000110344 or ENSG00000110395 or ENSG00000112130 or ENSG00000113269 or ENSG00000114062 or ENSG00000114423 or ENSG00000115392 or ENSG00000115760 or ENSG00000116514 or ENSG00000118518 or ENSG00000119048 or ENSG00000119401 or ENSG00000122257 or ENSG00000123124 or ENSG00000126107 or ENSG00000127481 or ENSG00000128731 or ENSG00000130725 or ENSG00000130939 or ENSG00000130985 or ENSG00000131508 or ENSG00000131653 or ENSG00000132256 or ENSG00000132388 or ENSG00000133135 or ENSG00000133606 or ENSG00000134758 or ENSG00000135679 or ENSG00000136536 or ENSG00000137393 or ENSG00000138376 or ENSG00000138411 or ENSG00000138641 or ENSG00000138942 or ENSG00000139266 or ENSG00000140367 or ENSG00000142273 or ENSG00000143207 or ENSG00000144357 or ENSG00000144583 or ENSG00000144744 or ENSG00000145416 or ENSG00000145495 or ENSG00000146373 or ENSG00000146414 or ENSG00000147854 or ENSG00000148356 or ENSG00000148634 or ENSG00000151148 or ENSG00000151692 or ENSG00000153827 or ENSG00000154370 or ENSG00000154447 or ENSG00000156463 or ENSG00000156587 or ENSG00000158022 or ENSG00000159202 or ENSG00000159459 or ENSG00000159461 or ENSG00000160087 or ENSG00000160714 or ENSG00000162298 or ENSG00000163012 or ENSG00000163162 or ENSG00000163481 or ENSG00000163743 or ENSG00000164068 or ENSG00000164197 or ENSG00000165338 or ENSG00000165406 or ENSG00000166349 or ENSG00000168159 or ENSG00000168411 or ENSG00000170035 or ENSG00000170142 or ENSG00000170881 or ENSG00000172985 or ENSG00000173838 or ENSG00000173926 or ENSG00000175063 or ENSG00000175809 or ENSG00000175931 or ENSG00000176641 or ENSG00000177414 or ENSG00000177889 or ENSG00000179455 or ENSG00000180233 or ENSG00000180537 or ENSG00000181191 or ENSG00000181788 or ENSG00000181852 or ENSG00000182179 or ENSG00000182247 or ENSG00000182670 or ENSG00000183654 or ENSG00000184182 or ENSG00000184787 or ENSG00000185345 or ENSG00000185651 or ENSG00000186187 or ENSG00000186591 or ENSG00000187566 or ENSG00000188050 or ENSG00000196470 or ENSG00000197323 or ENSG00000197530 or ENSG00000197579 or ENSG00000198060 or ENSG00000198373 or ENSG00000198742 or ENSG00000198833 or ENSG00000198919 or ENSG00000198961 or ENSG00000204308 or ENSG00000214357 or ENSG00000215218 or ENSG00000239305 or ENSG00000265491 or ENSG00000276043	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000103275 encodes a protein involved in the sumoylation process, which entails transferring ubiquitin-like proteins (SUMO) onto target proteins, which is necessary for nuclear architecture, chromosome segregation, and transcription-coupled nucleotide excision repair. However, the reaction provided describes a ubiquitination process involving ubiquitin attachment to a protein lysine residue, ATP consumption, and production of AMP and PPi, which is not related to the sumoylation functions of the gene ENSG00000103275.	No Yes
ENSG00000197506	Q9HAS3	MAR12460: H+ + allopurinol --> H+ + allopurinol	ENSG00000197506	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000197506 encodes a sodium-dependent nucleoside transporter involved in transporting nucleosides such as thymidine, inosine, cytidine, uridine, adenosine, and guanosine, as well as certain nucleoside analogs. The catalytic activities described involve the transport of these nucleosides across the membrane with a sodium ion gradient. However, the reaction provided (H+ + allopurinol --> H+ + allopurinol) involves allopurinol, which is not a nucleoside or a nucleoside analog, and therefore does not fall within the described function or catalytic activities of the gene product.	No
ENSG00000197506	Q9HAS3	MAR12471: Na+ + allopurinol --> Na+ + allopurinol	ENSG00000137860 or ENSG00000156222 or ENSG00000197506	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000197506 is involved in the sodium-dependent transport of nucleosides such as thymidine, uridine, cytidine, adenosine, guanosine, and inosine. It functions by employing a 2:1 sodium/nucleoside ratio and does not participate in the reaction involving allopurinol. The provided reaction with allopurinol does not match any of the catalyzed activities of the gene, making it unrelated.	No
ENSG00000156222	O00337	MAR12471: Na+ + allopurinol --> Na+ + allopurinol	ENSG00000137860 or ENSG00000156222 or ENSG00000197506	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000156222 codes for a sodium and pyrimidine nucleoside symporter that imports uridine, thymidine, cytidine, and adenosine into cells by coupling their transport to the transmembrane sodium electrochemical gradient. However, the provided reaction involves the transport of allopurinol, which is not a pyrimidine nucleoside and is not mentioned as a substrate for the symporter in the gene's annotation. Therefore, the gene does not relate to the reaction involving allopurinol.	No

[JHL-452b]

Aha, does this Archive in here have anything to do with this issue? @SagarChandra07

[thelabcat]

Aha, does this Archive in here have anything to do with this issue? @SagarChandra07

Do not run that file! It is malware. The account has been spamming links to it all over GitHub. They use a password protected zip archive to evade the auto-check by MediaFire. I extracted it on a non-Windows machine and checked it manually. 17 positives!

[JHL-452b]

That's too bad! 😕 I deleted the reply with the malicious link, thanks for your reminder.

[feiranl]

For MAR04201, retain the genes ENSG00000167419, ENSG00000117592, and ENSG00000130508. For MAR04202, retain ENSG00000198099, ENSG00000196616, and ENSG00000196344, noting that ADH7 has a broader substrate range. For MAR08752, retain ENSG00000117592 and ENSG00000167419. For MAR02531, retain ENSG00000117592. For MAR09534, retain ENSG00000165591. For MAR09491, retain ENSG00000103275, which is related to protein ubiquitination.

For MAR02195, MAR02197, and MAR02189, remove ENSG00000184254, but add a copy of those rxns with the change from NADPH to NAD to reflect NAD-dependent characteristics.

Good example for the manuscript:
ENSG00000168137 and ENSG00000243056 for MAR09464, totally wrong gene associations
ENSG00000162408 for MAR09466, totally wrong gene associations
ENSG00000221988 for MAR09499, because of structural constraints, is unable to remove palmitate from peptides or proteins as annotated in UniProt. AI is correct.
ENSG00000213722 for MAR09513. Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed:21493890, PubMed:37296100). AI is correct.
ENSG00000197506 for MAR12460, it is a sodium dependent transporter, thus the reaction provided (H+ + allopurinol --> H+ + allopurinol) is not correct.

[JHL-452b]

For MAR02195, MAR02197, and MAR02189, remove ENSG00000184254, but add a copy of those rxns with the change from NADPH to NAD to reflect NAD-dependent characteristics.

Agree to remove ENSG00000184254 from MAR02195, MAR02197 and MAR02189. However, a copy of those rxns with NAD has already in Human-GEM.

• MAR02192 is the copy of MAR02195 with NAD.
• MAR02196 is the copy of MAR02197 with NAD.
• MAR02188 is the copy of MAR02189 with NAD.

And all GPRs of these reactions include ENSG00000184254. So, there is no need to add a new copy reactions for MAR02195, MAR02197, and MAR02189.

[JHL-452b]

Fixed by #931