Skip to content

Commit

Permalink
update the Colab_Reproducibility:https
Browse files Browse the repository at this point in the history
  • Loading branch information
DBinary committed Nov 19, 2023
1 parent b0ba2a8 commit 66d66b3
Show file tree
Hide file tree
Showing 21 changed files with 68 additions and 43 deletions.
4 changes: 3 additions & 1 deletion omicverse_guide/docs/Tutorials-bulk/t_bulk_combat.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,9 @@
"\n",
"Paper: [pyComBat, a Python tool for batch effects correction in high-throughput molecular data using empirical Bayes methods](https://doi.org/10.1101/2020.03.17.995431)\n",
"\n",
"Code: https://github.com/epigenelabs/pyComBat"
"Code: https://github.com/epigenelabs/pyComBat\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/121bbIiI3j4pTZ3yA_5p8BRkRyGMMmNAq?usp=sharing"
]
},
{
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-bulk/t_deg.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -10,7 +10,7 @@
"\n",
"An important task of bulk rna-seq analysis is the different expression , which we can perform with omicverse. For different expression analysis, ov change the `gene_id` to `gene_name` of matrix first. When our dataset existed the batch effect, we can use the SizeFactors of DEseq2 to normalize it, and use `t-test` of `wilcoxon` to calculate the p-value of genes. Here we demonstrate this pipeline with a matrix from `featureCounts`. The same pipeline would generally be used to analyze any collection of RNA-seq tasks. \n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/19MyE4hNu_PRtz7Yvhs3VIOR8VMc9PkyI?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/1q5lDfJepbtvNtc1TKz-h4wGUifTZ3i0_?usp=sharing"
]
},
{
Expand Down
12 changes: 11 additions & 1 deletion omicverse_guide/docs/Tutorials-bulk/t_deseq2.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -16,7 +16,7 @@
"\n",
"Code: https://github.com/owkin/PyDESeq2\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1jX73eidBkDqGpd6qLASIjGENQaeZ34EU?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/1fZS-v0zdIYkXrEoIAM1X5kPoZVfVvY5h?usp=sharing"
]
},
{
Expand Down Expand Up @@ -224,6 +224,16 @@
"We performed the gene_id mapping by the mapping pair file `GRCm39` downloaded before."
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "5eda606e",
"metadata": {},
"outputs": [],
"source": [
"ov.utils.download_geneid_annotation_pair()"
]
},
{
"cell_type": "code",
"execution_count": 3,
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-bulk/t_network.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@
"\n",
"Here we produce a tutorial that use python to construct protein-protein interaction network\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1q0_ikTYYscphDW_LUGZkplN94yAQ2CvU?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/1ReLCFA5cNNcem_WaMXYN9da7W0GN4gzl?usp=sharing"
]
},
{
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-bulk/t_wgcna.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -13,7 +13,7 @@
"\n",
"Code: Reproduce by Python. Raw is http://www.genetics.ucla.edu/labs/horvath/CoexpressionNetwork/Rpackages/WGCNA\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1XUspbEMX9aOS96RPMkfmb9mi9H7lZl1F?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1EbP-Tq1IwYO9y1_-zzw23XlPbzrxP0og?usp=sharing\n",
"\n",
"Here, you will be briefly guided through the basics of how to use omicverse to perform wgcna anlysis. Once you are set"
]
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -13,7 +13,7 @@
"\n",
"Code: https://github.com/ZJUFanLab/bulk2space\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1He71hAyeAv1DHQyXUlxtoJ4QvwZwW7I0?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1ST5tvSH3lqMkXLf8xv8jpPMHCWO8KJOC?usp=sharing\n",
"\n",
"This tutorial walks through how to read, set-up and train the model from bulk RNA-seq and reference scRNA-seq data. We use the pdac datasets as example"
]
Expand Down Expand Up @@ -489,7 +489,7 @@
}
],
"source": [
"GEP,CellFractionPrediction=model.predicted_fraction()"
"CellFractionPrediction=model.predicted_fraction()"
]
},
{
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@
"\n",
"We developed BulkTrajBlend in OmicVerse, which is specifically designed to address cell continuity in single-cell sequencing.BulkTrajBlend first deconvolves single-cell data from Bulk RNA-seq and then uses a GNN-based overlapping community discovery algorithm to identify contiguous cells in the generated single-cell data.\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1HulVXQIlUEcpGRDZo4MxcHYOjnVhuCC-?usp=sharing\n"
"Colab_Reproducibility:https://colab.research.google.com/drive/1JChlTjcejvZydZleMGvsJPKgSG4TdrB-?usp=sharing\n"
]
},
{
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -14,7 +14,7 @@
"\n",
"Code: https://github.com/ZJUFanLab/bulk2space\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1oPvPpNnt2YF803CQNdH_7PdRuPqtKRVb?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1E3TlGUW2bxEew8JYCLE9qA6saYbIjBnY?usp=sharing\n",
"\n",
"This tutorial walks through how to read, set-up and train the model from scRNA-seq and reference stRNA-seq data. We use the pdac datasets as example"
]
Expand Down
12 changes: 7 additions & 5 deletions omicverse_guide/docs/Tutorials-single/t_anno_trans.ipynb

Large diffs are not rendered by default.

2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_aucell.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -16,7 +16,7 @@
"\n",
"Code: https://github.com/aertslab/AUCell\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1d0nvAyP6Jo2Be7NdX00aHD39YZubDdeX?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1Rk7Zopil-Ve1WFRQLV_AZOwCHRyhsOXk?usp=sharing\n",
"\n",
"<div class=\"admonition warning\">\n",
" <p class=\"admonition-title\">Warning</p>\n",
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_cellanno.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -14,7 +14,7 @@
"\n",
"Code: https://github.com/bioinfo-ibms-pumc/SCSA\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1RO6giAKFEsleaGAN8YIiA6M0QPJlhJuY?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1BC6hPS0CyBhNu0BYk8evu57-ua1bAS0T?usp=sharing\n",
"\n",
"<div class=\"admonition warning\">\n",
" <p class=\"admonition-title\">Note</p>\n",
Expand Down
29 changes: 15 additions & 14 deletions omicverse_guide/docs/Tutorials-single/t_cellfate_gene.ipynb

Large diffs are not rendered by default.

16 changes: 9 additions & 7 deletions omicverse_guide/docs/Tutorials-single/t_cellfate_genesets.ipynb

Large diffs are not rendered by default.

4 changes: 3 additions & 1 deletion omicverse_guide/docs/Tutorials-single/t_cluster.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,9 @@
"\n",
"## Preprocess data\n",
"\n",
"As an example, we apply differential kinetic analysis to dentate gyrus neurogenesis, which comprises multiple heterogeneous subpopulations."
"As an example, we apply differential kinetic analysis to dentate gyrus neurogenesis, which comprises multiple heterogeneous subpopulations.\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1d_szq-y-g7O0C5rJgK22XC7uWTUNrYpK?usp=sharing"
]
},
{
Expand Down
2 changes: 2 additions & 0 deletions omicverse_guide/docs/Tutorials-single/t_metatime.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -15,6 +15,8 @@
"\n",
"Code: https://github.com/yi-zhang/MetaTiME\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1isvjTfSFM2cy6GzHWAwbuvSjveEJijzP?usp=sharing\n",
"\n",
"![metatime](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41467-023-38333-8/MediaObjects/41467_2023_38333_Fig1_HTML.png)"
]
},
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_mofa.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -15,7 +15,7 @@
"\n",
"Code: https://github.com/bioFAM/mofapy2\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1Grp-r-fQ3LHO7myJt4r_fPX7T3uJ-vBy?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/1UPGQA3BenrC-eLIGVtdKVftSnOKIwNrP?usp=sharing"
]
},
{
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_mofa_glue.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,7 @@
"\n",
"Code: https://github.com/bioFAM/mofapy2 and https://github.com/gao-lab/GLUE\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1_rickQz7irTremCZytnnNQTQLyhgBn0O?usp=sharing\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1zlakFf20IoBdyuOQDocwFQHu8XOVizRL?usp=sharing\n",
"\n",
"We used the result anndata object `rna-emb.h5ad` and `atac.emb.h5ad` from [GLUE'tutorial](https://scglue.readthedocs.io/en/latest/training.html)"
]
Expand Down
2 changes: 2 additions & 0 deletions omicverse_guide/docs/Tutorials-single/t_nocd.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -11,6 +11,8 @@
"\n",
"This tutorial focuses on how to cluster the cell with overlapping and identify the cell with multi-fate\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1l7iHdVmTQcv9YmpIjhK_UzLuHbJ1Jv9E?usp=sharing\n",
"\n",
"<div class=\"admonition warning\">\n",
" <p class=\"admonition-title\">Warning</p>\n",
" <p>\n",
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_preprocess.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -13,7 +13,7 @@
"\n",
"User can compare our tutorial with [scanpy'tutorial](https://scanpy-tutorials.readthedocs.io/en/latest/pbmc3k.html) to learn how to use omicverse well\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/18a6vsvdRdWVPCP-KSaiznou7PBVtNVXw?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/1DXLSls_ppgJmAaZTUvqazNC_E7EDCxUe?usp=sharing"
]
},
{
Expand Down
2 changes: 1 addition & 1 deletion omicverse_guide/docs/Tutorials-single/t_scdeg.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@
"Sometimes we need to compare differentially expressed genes or differentially expressed features between two cell types on single cell data, but existing methods focus more on cell-specific gene analysis. Researchers need to transfer bulk RNA-seq analysis to single-cell analysis, which involves interaction between different programming languages or programming tools, adding significantly to the workload of the researcher.\n",
"Here, we use omicverse's bulk RNA-seq pyDEG method to complete differential expression analysis at the single cell level.\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/1u6digKEpN7m88iA0_668UZLvlqYwypny?usp=sharing"
"Colab_Reproducibility:https://colab.research.google.com/drive/12faBRh0xT7v6KSy8NCSRqbegF_AEoDXr?usp=sharing"
]
},
{
Expand Down
4 changes: 3 additions & 1 deletion omicverse_guide/docs/Tutorials-single/t_traj.ipynb
Original file line number Diff line number Diff line change
Expand Up @@ -16,7 +16,9 @@
"\n",
"## Preprocess data\n",
"\n",
"As an example, we apply differential kinetic analysis to dentate gyrus neurogenesis, which comprises multiple heterogeneous subpopulations."
"As an example, we apply differential kinetic analysis to dentate gyrus neurogenesis, which comprises multiple heterogeneous subpopulations.\n",
"\n",
"Colab_Reproducibility:https://colab.research.google.com/drive/18aFCEmuiYxH5BDS3H7SkemmL2isG7iL2?usp=sharing"
]
},
{
Expand Down

0 comments on commit 66d66b3

Please sign in to comment.