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Added fastq_trim_fastp_fastqc
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GallVp committed Feb 23, 2024
1 parent 43255b6 commit 1589033
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1 change: 0 additions & 1 deletion README.md
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Expand Up @@ -73,7 +73,6 @@ Prepare an `assemblysheet.csv` file with following columns representing target a
- `fasta:` FASTA file
- `gff3 [Optional]:` GFF3 annotation file if available
- `monoploid_ids [Optional]:` A txt file listing the IDs used to calculate LAI in monoploid mode if necessary
- `hic_reads [Optional]:` A SRA id such as 'SRR8238190' or path to paired reads such as 'PG_PETUNIA_HiC_CGYCF_CACTCA_L001_R{1,2}.fastq.gz'
- `synteny_labels [Optional]:` A two column tsv file listing fasta sequence ids (first column) and labels for the synteny plots (second column) when performing synteny analysis

Now, you can run the pipeline using:
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4 changes: 2 additions & 2 deletions assets/assemblysheet.csv
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@@ -1,2 +1,2 @@
tag,fasta,gff3,monoploid_ids,hic_reads,synteny_labels
FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,,
tag,fasta,gff3,monoploid_ids,synteny_labels
FI1,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/003/814/445/GCA_003814445.1_ASM381444v1/GCA_003814445.1_ASM381444v1_genomic.gff.gz,,
17 changes: 0 additions & 17 deletions assets/schema_input.json
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Expand Up @@ -35,23 +35,6 @@
}
]
},
"hic_reads": {
"errorMessage": "HiC reads should either be provided as a SRA ID or as a path to paired reads with pattern '*R{1,2}.(fastq|fq).gz'",
"anyOf": [
{
"type": "string",
"pattern": "^SR\\w+$"
},
{
"type": "string",
"pattern": "^\\S+R\\{1,2\\}\\.f(ast)?q\\.gz$"
},
{
"type": "string",
"maxLength": 0
}
]
},
"synteny_labels": {
"errorMessage": "Synteny labels tsv path cannot contain spaces and must have extension '.tsv'",
"anyOf": [
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15 changes: 15 additions & 0 deletions modules.json
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Expand Up @@ -90,6 +90,16 @@
"git_sha": "89ff95427f695086369d7927a3c17cea2a37a382",
"installed_by": ["modules"]
},
"fastp": {
"branch": "master",
"git_sha": "003920c7f9a8ae19b69a97171922880220bedf56",
"installed_by": ["fastq_trim_fastp_fastqc"]
},
"fastqc": {
"branch": "master",
"git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
"installed_by": ["fastq_trim_fastp_fastqc"]
},
"gunzip": {
"branch": "master",
"git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
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"branch": "master",
"git_sha": "2b21fbeb20ad9f17612f4a3dd7b12971513f08d5",
"installed_by": ["subworkflows"]
},
"fastq_trim_fastp_fastqc": {
"branch": "master",
"git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f",
"installed_by": ["subworkflows"]
}
}
}
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7 changes: 7 additions & 0 deletions modules/nf-core/fastp/environment.yml

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120 changes: 120 additions & 0 deletions modules/nf-core/fastp/main.nf

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75 changes: 75 additions & 0 deletions modules/nf-core/fastp/meta.yml

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