You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi, I am trying to run virus breakend on bwa mem output (it is a sorted bam file). I have generated the bam index using samtools index.
I pulled gridss image using docker pull gridss/gridss:latest
Then I enter the docker image through docker run -v $(pwd):/home/bleh -it gridss/gridss:latest
Next, I run virusbreakend: virusbreakend --kraken2db /home/bleh/virusbreakenddb_20210401 --output output.vcf --reference /home/bleh/fastvifi_ref/data_repo/hg19/hg19full.fa -j /home/bleh/gridss-2.13.2-gridss-jar-with-dependencies.jar /home/bleh/data/hg19_map/sortbwaB5.bam
I get the following error after the calling structural variants step:
Exit status 0 for $timecmd gridss -w $adjusteddir -t $threads -r $prefix_adjusted.viral.fa -j $gridss_jar -o $file_gridss_vcf -a $file_assembly -c $file_gridss_configuration --maxcoverage $maxcoverage $gridssargs $bam_list_args 1>&2 2>> $logfile
See log file ./output.vcf.virusbreakend.working/virusbreakend.20230902_090310.ef4dd93274b1.10.log for underlying error
The above log file asks me to refer to another log file at ./output.vcf.virusbreakend.working/adjusted/gridss.full.20230902_090418.ef4dd93274b1.1155.log gridss.full.20230902_090418.ef4dd93274b1.1155.log
Inside this log file it says at line 307
ERROR 2023-09-02 09:04:22 VariantCaller Fatal error during breakpoint identification
java.util.concurrent.ExecutionException: htsjdk.samtools.SAMException: No index is available for this BAM file.
But I have already created the bam index. I have modified the permissions of the index file as well with chmod 777
Any help is much appreciated.
The text was updated successfully, but these errors were encountered:
Hi, I am trying to run virus breakend on
bwa mem
output (it is a sorted bam file). I have generated the bam index usingsamtools index
.I pulled gridss image using
docker pull gridss/gridss:latest
Then I enter the docker image through
docker run -v $(pwd):/home/bleh -it gridss/gridss:latest
Next, I run virusbreakend:
virusbreakend --kraken2db /home/bleh/virusbreakenddb_20210401 --output output.vcf --reference /home/bleh/fastvifi_ref/data_repo/hg19/hg19full.fa -j /home/bleh/gridss-2.13.2-gridss-jar-with-dependencies.jar /home/bleh/data/hg19_map/sortbwaB5.bam
I get the following error after the calling structural variants step:
virusbreakend.20230902_090310.ef4dd93274b1.10.log
The above log file asks me to refer to another log file at
./output.vcf.virusbreakend.working/adjusted/gridss.full.20230902_090418.ef4dd93274b1.1155.log
gridss.full.20230902_090418.ef4dd93274b1.1155.log
Inside this log file it says at line 307
But I have already created the bam index. I have modified the permissions of the index file as well with
chmod 777
Any help is much appreciated.
The text was updated successfully, but these errors were encountered: