diff --git a/README.md b/README.md index dc8f128..dcf9363 100644 --- a/README.md +++ b/README.md @@ -352,6 +352,9 @@ Detailed Usage process_with_bam: processing from bam file input: bam file for aggregated data, outputted from the mapping module output: filtered peak-by-cell matrix and all intermediate results + rmDoublets: remove potential doublets + input: a peak-by-cell matrix file or a seurat object file in .rds format + output: doublets removed matrix.rds and barcodes.txt file and seurat objects w/ and w/o doublets saved in the input directory (and a umap plot colored by singlet/doubet) clustering: cell clustering input: filtered peak-by-cell matrix file, outputted from the call_cell module (or a seurat.rds file) output: seurat objects with clustering label in the metadata (.rds file) and @@ -421,9 +424,6 @@ Detailed Usage visualize: interactively visualize the data through VisCello input: VisCello_obj directory, outputted from the clustering module output: launch VisCello through web browser for interactively visualization" - rmDoublets: remove potential doublets - input: a peak-by-cell matrix file or a seurat object file in .rds format - output: doublets removed matrix.rds and barcodes.txt file and seurat objects w/ and w/o doublets saved in the input directory (and a umap colored by singlet/doubet) addCB2bam: add cell barcode tag to bam file input: a bam file generated by scATAC-pro diff --git a/scripts/dex_fastq_single.sh b/scripts/dex_fastq_single.sh index 26a9ece..a9841e1 100755 --- a/scripts/dex_fastq_single.sh +++ b/scripts/dex_fastq_single.sh @@ -65,7 +65,7 @@ else ${output_dir}/tmp${i}_demplxed_${dex_prefix1} ${fastqs[$i]} & ${PYTHON_PATH}/python ${curr_dir}/src/dex_fastq_extraIndex.py ${output_dir}/demplxed_${dex_prefix2} \ ${output_dir}/tmp${i}_demplxed_${dex_prefix2} ${fastqs[$i]} & - + wait mv ${output_dir}/tmp${i}_demplxed_${dex_prefix1} ${output_dir}/demplxed_${dex_prefix1} mv ${output_dir}/tmp${i}_demplxed_${dex_prefix2} ${output_dir}/demplxed_${dex_prefix2} done