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civibe-pupilprep

PLEASE READ BEFORE STARTING DATA PROCESSING.

This is a Python repository for preprocessing pupillometry data. It is licensed under GPL-3.

The data this was developed for was recorded using the retinaWISE device. Dominant eye was stimulated for the following conditions: flux, mel, lms, l-m, s. The experiment consists of 11 blocks, each with 2 recordings: test a and b, spaced over 40 hours.

The protocol for one recording was as follows:

  1. 4 minutes adaptation sequence.
  2. 18 seconds sequence composed of: 5 s condition-based stim, 13 s baseline stim.

In each test there was one adaptation sequence, and 5 condition sequences for each condition. The pre-stimulation phase which was used as baseline comes from 1 second period before stimulation onset (taken from the previous sequence).

For the purpose of analysis, using this preprocessing pipeline the data is resampled to 30 Hz and segmented into 19 s long trials, lasting from -1 to 18 s. The artefacts are removed based on non-physiological pupil size outside of 1.5 - 9 mm range (pre-resampling), MAD pupil velocity thresholding (post-resampling), MAD pupil size thresholding (post-resampling, post-velocity thresholding). As the data is already sparse and underwent smoothing in device, we limit artefact removal to minimum.

Installation

It's recommended you install requirements directly if you're not sure you have the wheel package. To do so, run:

pip install -r requirements.txt

To get the package, first you need to build a wheel for it. Run this in the repository folder:

python setup.py bdist_wheel

Then install the package from the wheel. To do so, run:

pip install .\dist\wheel-name-here.whl

For instance, for version 0.1.1 the wheel name is civibe_pupilprep_utils-0.1.1-py3-none-any.whl. Adjust formatting as needed (the above is for Windows). The package and dependencies should now be installed.

Then you can import the package for instance as:

import pupilprep_utilities

print(pupilprep_utilities.preprocessing_utils.resample_by_trial.__doc__)

or better import modules you need as:

import pupilprep_utilities.preprocessing_utils as prep

print(prep.resample_by_trial.__doc__)

Scripts to run before running the notebooks

Run fully_preprocess_all.py with saving intermediate steps enabled to get data from all preprocessing steps.

  1. load_and_resample: get raw and resampled data. By default, data is resampled to 30 Hz and cut to -1:18 s trial segments.
  2. remove_artefacts: perform artefact removal on the resampled data. By default, it performs thresholding based on rolling pupil velocity MAD and rolling pupil size MAD.
  3. reject_incomplete: perform trial/block rejection on resampled and cleaned/ or just resampled data. By default the period of interest is 0:6 s with min. 75% completeness, baseline is -1:0 s with min. 40% completeness, no long NaN > 625 ms. A block is valid for the condition if it has min. 3 trials for that condition and flux.

Notebooks explanation

load_ - notebooks relating to loading the data from raw experiment files

prep_ - notebooks relating to developing functions for data preprocessing

eda_ - notebooks with exploratory data analysis relating to e.g. exploration of the recording details, determination of thresholds for data acceptance, statistics of data completeness

All notebooks have a section 'Purpose of the notebook' which explains which data they use, from which script.

Utilities explanation

loading_utils - utilities for loading and marking data from raw files based on protocol

preprocessing_utils - utilities for preprocessing data from dataframes created with loading_utils, as in: resampling, artefact removal, trial/block rejection

visualisation_utils - utilities for plotting data

pipelines - module with pipelines for pupillometry preprocessing

analysis_utils - utilities for data analysis after preprocessing