Parchman lab chemical and plastic inventory / project workflow timelines

TO ORDER

2 MseI 2-3 T4 2-3 large Iproof 2-3 dNTPs

All oligos fresh

Plastics

Listed by category and order number below

BioRad plates and caps

Order confirmations: 1006420777
Plates: Multiplate™ 96-Well PCR Plates, high profile, unskirted, clear #MLP9601
Caps: TCS0801

Tips

We mostly go through a large volume of 10 μl filtered tips.

10 μl filtered tips VWR NA cat number: 76322-132 (case is 5 boxes of 10 racks; $247.23)
order: 1-800-932-5000 7:30 a.m. to 8:00 p.m. (EST)

Reagents:

NEB

1-800-632-5227 (Monday - Friday 9:00 AM - 8:00 PM EST)

T4 buffer B0202S 10 X 5 ml $27.00
EcoRI (NEB, 20,000 units/mL) R0101L
MseI (NEB, 10,000 units/mL) R0525L
T4 DNA ligase (NEB, 400,000 units/mL) M0202L

500 bp ladder: N3231S

Promega

DNTPS (U1515)
order: (608) 274-4330

BioRad

iproof polymerase: 2 units/μl, 250 μl 500U 172-5302
order: 1-800-4-BIORAD (1-800-424-6723)

Project workflow - on deck

Eriogonum umbellatum

~700 samples, top freezer- check DNA

Fraxinus velutina

Chestnut

Erigeron