From 661537179402675cc75949833ed4ed451d42503d Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 7 Oct 2022 17:00:55 +0100
Subject: [PATCH 01/85] .first() is not needed

---
 subworkflows/local/params_check.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/params_check.nf b/subworkflows/local/params_check.nf
index e1fcf63..3b7610b 100644
--- a/subworkflows/local/params_check.nf
+++ b/subworkflows/local/params_check.nf
@@ -31,7 +31,7 @@ workflow PARAMS_CHECK {
             ] }
             .set { ch_inputs }
 
-        ch_versions = ch_versions.mix(SAMPLESHEET_CHECK.out.versions.first())
+        ch_versions = ch_versions.mix(SAMPLESHEET_CHECK.out.versions)
 
     } else {
 

From cf0e48fb35b9a40dffd492f71f7cfb96310fa355 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 8 Oct 2022 10:48:58 +0100
Subject: [PATCH 02/85] Version bump

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 8263dc8..93c7650 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -173,7 +173,7 @@ manifest {
     description     = 'Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure'
     mainScript      = 'main.nf'
     nextflowVersion = '!>=22.04.0'
-    version         = '1.1.0'
+    version         = '1.2dev'
 }
 
 // Load modules.config for DSL2 module specific options

From b63474ec06f72931bebd9278a9b74fc832ad7d26 Mon Sep 17 00:00:00 2001
From: Guoying Qi <gq2@sanger.ac.uk>
Date: Sat, 3 Dec 2022 13:34:18 +0000
Subject: [PATCH 03/85] add missing light logo image in assets directory

---
 assets/sanger-tol-insdcdownload_logo_light.png | Bin 0 -> 159422 bytes
 assets/sendmail_template.txt                   |   2 +-
 2 files changed, 1 insertion(+), 1 deletion(-)
 create mode 100755 assets/sanger-tol-insdcdownload_logo_light.png

diff --git a/assets/sanger-tol-insdcdownload_logo_light.png b/assets/sanger-tol-insdcdownload_logo_light.png
new file mode 100755
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diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt
index 7e07ad0..6703a88 100644
--- a/assets/sendmail_template.txt
+++ b/assets/sendmail_template.txt
@@ -9,7 +9,7 @@ Content-Type: text/html; charset=utf-8
 $email_html
 
 --nfcoremimeboundary
-Content-Type: image/png;name="sanger-tol-insdcdownload_logo.png"
+Content-Type: image/png;name="sanger-tol-insdcdownload_logo_light.png"
 Content-Transfer-Encoding: base64
 Content-ID: <nfcorepipelinelogo>
 Content-Disposition: inline; filename="sanger-tol-insdcdownload_logo_light.png"

From cd0c71054e002eef5ebd9b0298954bb1f1d0a30e Mon Sep 17 00:00:00 2001
From: Guoying Qi <gq2@sanger.ac.uk>
Date: Sat, 3 Dec 2022 13:35:18 +0000
Subject: [PATCH 04/85] resize logo image size in html email

---
 assets/email_template.html | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/assets/email_template.html b/assets/email_template.html
index d63771d..01675b1 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -10,7 +10,7 @@
 <body>
 <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
 
-<img src="cid:nfcorepipelinelogo">
+<img src="cid:nfcorepipelinelogo" width="35%">
 
 <h1>sanger-tol/insdcdownload v${version}</h1>
 <h2>Run Name: $runName</h2>

From ba63cebd6890bfafe18069f915fc4669ccaa6282 Mon Sep 17 00:00:00 2001
From: Guoying Qi <gq2@sanger.ac.uk>
Date: Sun, 4 Dec 2022 12:05:42 +0000
Subject: [PATCH 05/85] reduce the logo image furthrer and show the full size
 in the email html template

---
 assets/email_template.html                    |   2 +-
 .../sanger-tol-insdcdownload_logo_light.png   | Bin 159422 -> 102793 bytes
 2 files changed, 1 insertion(+), 1 deletion(-)

diff --git a/assets/email_template.html b/assets/email_template.html
index 01675b1..d63771d 100644
--- a/assets/email_template.html
+++ b/assets/email_template.html
@@ -10,7 +10,7 @@
 <body>
 <div style="font-family: Helvetica, Arial, sans-serif; padding: 30px; max-width: 800px; margin: 0 auto;">
 
-<img src="cid:nfcorepipelinelogo" width="35%">
+<img src="cid:nfcorepipelinelogo">
 
 <h1>sanger-tol/insdcdownload v${version}</h1>
 <h2>Run Name: $runName</h2>
diff --git a/assets/sanger-tol-insdcdownload_logo_light.png b/assets/sanger-tol-insdcdownload_logo_light.png
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From 6c418edb05aafffcfb967187c794968a2fd366a5 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 7 Dec 2022 15:26:24 +0000
Subject: [PATCH 06/85] Create tower_lsf_full_tests.yml

---
 .github/workflows/tower_lsf_full_tests.yml | 31 ++++++++++++++++++++++
 1 file changed, 31 insertions(+)
 create mode 100644 .github/workflows/tower_lsf_full_tests.yml

diff --git a/.github/workflows/tower_lsf_full_tests.yml b/.github/workflows/tower_lsf_full_tests.yml
new file mode 100644
index 0000000..0b799af
--- /dev/null
+++ b/.github/workflows/tower_lsf_full_tests.yml
@@ -0,0 +1,31 @@
+name: nf-core LSF full size tests
+
+on:
+  pull_request:
+    branches:
+      - main
+      - dev
+jobs:
+  run-tower:
+    name: Run LSF full size tests
+    runs-on: ubuntu-latest
+    steps:
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@v2
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.event.pull_request.head.sha }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.event.pull_request.head.sha }}
+          parameters: |
+            {
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.event.pull_request.head.sha }}",
+              "email": "${{ secrets.TOWER_RUN_EMAIL }}",
+              "email_on_fail": "${{ secrets.TOWER_RUN_EMAIL }}"
+            }
+          profiles: test_full,sanger,singularity
+          nextflow_config: |
+            process.errorStrategy = 'retry'
+            process.maxRetries = 3

From 33e89e36c943d2c3bf6967bbc887181777c69809 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Thu, 8 Dec 2022 09:13:46 +0000
Subject: [PATCH 07/85] Update tower_lsf_full_tests.yml

Remove email for notification and retry
---
 .github/workflows/tower_lsf_full_tests.yml | 5 -----
 1 file changed, 5 deletions(-)

diff --git a/.github/workflows/tower_lsf_full_tests.yml b/.github/workflows/tower_lsf_full_tests.yml
index 0b799af..749995e 100644
--- a/.github/workflows/tower_lsf_full_tests.yml
+++ b/.github/workflows/tower_lsf_full_tests.yml
@@ -22,10 +22,5 @@ jobs:
           parameters: |
             {
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.event.pull_request.head.sha }}",
-              "email": "${{ secrets.TOWER_RUN_EMAIL }}",
-              "email_on_fail": "${{ secrets.TOWER_RUN_EMAIL }}"
             }
           profiles: test_full,sanger,singularity
-          nextflow_config: |
-            process.errorStrategy = 'retry'
-            process.maxRetries = 3

From 6a8820f9712b00f0641a054b7fb2877726a15b76 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Thu, 8 Dec 2022 17:28:47 +0000
Subject: [PATCH 08/85] Update and rename tower_lsf_full_tests.yml to
 sangerfulltest.yml

only run when release or manual
---
 ...ower_lsf_full_tests.yml => sangerfulltest.yml} | 15 +++++++--------
 1 file changed, 7 insertions(+), 8 deletions(-)
 rename .github/workflows/{tower_lsf_full_tests.yml => sangerfulltest.yml} (69%)

diff --git a/.github/workflows/tower_lsf_full_tests.yml b/.github/workflows/sangerfulltest.yml
similarity index 69%
rename from .github/workflows/tower_lsf_full_tests.yml
rename to .github/workflows/sangerfulltest.yml
index 749995e..308e77a 100644
--- a/.github/workflows/tower_lsf_full_tests.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -1,10 +1,9 @@
-name: nf-core LSF full size tests
+name: nf-core Sanger LSF full size tests
 
 on:
-  pull_request:
-    branches:
-      - main
-      - dev
+  release:
+    types: [published]
+  workflow_dispatch:
 jobs:
   run-tower:
     name: Run LSF full size tests
@@ -17,10 +16,10 @@ jobs:
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
-          revision: ${{ github.event.pull_request.head.sha }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.event.pull_request.head.sha }}
+          revision: ${{ github.sha }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.event.pull_request.head.sha }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.sha }}",
             }
           profiles: test_full,sanger,singularity

From afd58b623fa549dd39ca7164e070c1ca77970eab Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Thu, 8 Dec 2022 17:30:38 +0000
Subject: [PATCH 09/85] Create sangertest.yml

to manaully run test on Sanger LSF
---
 .github/workflows/sangertest.yml | 23 +++++++++++++++++++++++
 1 file changed, 23 insertions(+)
 create mode 100644 .github/workflows/sangertest.yml

diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sangertest.yml
new file mode 100644
index 0000000..13a0867
--- /dev/null
+++ b/.github/workflows/sangertest.yml
@@ -0,0 +1,23 @@
+name: nf-core Sanger LSF tests
+
+on:
+  workflow_dispatch:
+jobs:
+  run-tower:
+    name: Run LSF tests
+    runs-on: ubuntu-latest
+    steps:
+      - name: Launch workflow via tower
+        uses: nf-core/tower-action@v2
+        with:
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          pipeline: ${{ github.repository }}
+          revision: ${{ github.sha }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.sha }}",
+            }
+          profiles: test,sanger,singularity

From f0f1c95eae7e73d0e6bd2953e74ceef1f05d1d7b Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Mon, 12 Dec 2022 15:13:58 +0000
Subject: [PATCH 10/85] Update sangerfulltest.yml

trigger the full test workflow when push to main or dev
---
 .github/workflows/sangerfulltest.yml | 23 ++++++++++++++++++-----
 1 file changed, 18 insertions(+), 5 deletions(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index 308e77a..971cb24 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -1,14 +1,27 @@
 name: nf-core Sanger LSF full size tests
 
 on:
-  release:
-    types: [published]
+  push:
+    branches:
+      - main
+      - dev
   workflow_dispatch:
 jobs:
   run-tower:
     name: Run LSF full size tests
     runs-on: ubuntu-latest
     steps:
+
+      - name: Sets env vars for push
+        run: |
+          echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV
+        if: github.event_name == 'pull_request'
+
+      - name: Sets env vars for workflow_dispatch
+        run: |
+          echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV
+        if: github.event_name == 'workflow_dispatch'
+
       - name: Launch workflow via tower
         uses: nf-core/tower-action@v2
         with:
@@ -16,10 +29,10 @@ jobs:
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
-          revision: ${{ github.sha }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.sha }}
+          revision: ${{ env.REVISION }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ env.REVISION }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.sha }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ env.REVISION }}",
             }
           profiles: test_full,sanger,singularity

From 10416fcae0c3063048c62a2efb47f166daaeb482 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Mon, 12 Dec 2022 15:57:36 +0000
Subject: [PATCH 11/85] Update sangerfulltest.yml

update the wrong event name for push
---
 .github/workflows/sangerfulltest.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index 971cb24..dbf8caa 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -15,7 +15,7 @@ jobs:
       - name: Sets env vars for push
         run: |
           echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV
-        if: github.event_name == 'pull_request'
+        if: github.event_name == 'push'
 
       - name: Sets env vars for workflow_dispatch
         run: |

From 2bacd5ebe3f32db88954b94d281e40618645cbab Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Tue, 13 Dec 2022 10:38:43 +0000
Subject: [PATCH 12/85] Update sangerfulltest.yml

update the work and results directory name
---
 .github/workflows/sangerfulltest.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index dbf8caa..b40dd66 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -30,9 +30,9 @@ jobs:
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ env.REVISION }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ env.REVISION }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/insdcdownload/work-${{ env.REVISION }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ env.REVISION }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/insdcdownload/results-${{ env.REVISION }}",
             }
           profiles: test_full,sanger,singularity

From be8188027f08b255f89ace2c782cc1059bbb04ce Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Tue, 13 Dec 2022 10:39:33 +0000
Subject: [PATCH 13/85] Update sangertest.yml

update outdir and work directory name
---
 .github/workflows/sangertest.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sangertest.yml
index 13a0867..c0c89b2 100644
--- a/.github/workflows/sangertest.yml
+++ b/.github/workflows/sangertest.yml
@@ -15,9 +15,9 @@ jobs:
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/readmapping/work-${{ github.sha }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/insdcdownload/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/readmapping/results-${{ github.sha }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/insdcdownload/results-${{ github.sha }}",
             }
           profiles: test,sanger,singularity

From af7588a0adb62985ad9990dd10d1d4bb93620f70 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 14 Dec 2022 11:51:30 +0000
Subject: [PATCH 14/85] Update sangertest.yml

using variables for out and work dir
---
 .github/workflows/sangertest.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sangertest.yml
index c0c89b2..f9befb3 100644
--- a/.github/workflows/sangertest.yml
+++ b/.github/workflows/sangertest.yml
@@ -15,9 +15,9 @@ jobs:
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/insdcdownload/work-${{ github.sha }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/${{ github.repository }}/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/insdcdownload/results-${{ github.sha }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ github.sha }}",
             }
           profiles: test,sanger,singularity

From 91dcc6aee208958cd0ba909d3bf95af34b6919e3 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 14 Dec 2022 11:52:11 +0000
Subject: [PATCH 15/85] Update sangerfulltest.yml

using variable for our and work dir
---
 .github/workflows/sangerfulltest.yml | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index b40dd66..e89f0dd 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -30,9 +30,9 @@ jobs:
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           pipeline: ${{ github.repository }}
           revision: ${{ env.REVISION }}
-          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/insdcdownload/work-${{ env.REVISION }}
+          workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/${{ github.repository }}/work-${{ env.REVISION }}
           parameters: |
             {
-              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/insdcdownload/results-${{ env.REVISION }}",
+              "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ env.REVISION }}",
             }
           profiles: test_full,sanger,singularity

From 858714be84ff86f9a2656bf64b3e7ebad3572dae Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:08:34 +0000
Subject: [PATCH 16/85] Updated logo

---
 lib/NfcoreTemplate.groovy | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 78966e9..835aecc 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -223,12 +223,12 @@ class NfcoreTemplate {
         String.format(
             """\n
             ${dashedLine(monochrome_logs)}
-            ${colors.blue}   _____                                ${colors.green} _______   ${colors.red} _${colors.reset}
-            ${colors.blue}  / ____|                               ${colors.green}|__   __|  ${colors.red}| |${colors.reset}
-            ${colors.blue} | (___   __ _ _ __   __ _  ___ _ __ ${colors.reset}______${colors.green}| |${colors.yellow} ___ ${colors.red}| |${colors.reset}
-            ${colors.blue}  \\___ \\ / _` | '_ \\ / _` |/ _ \\ '__|${colors.reset}______${colors.green}| |${colors.yellow}/ _ \\${colors.red}| |${colors.reset}
-            ${colors.blue}  ____) | (_| | | | | (_| |  __/ |         ${colors.green}| |${colors.yellow} (_) ${colors.red}| |____${colors.reset}
-            ${colors.blue} |_____/ \\__,_|_| |_|\\__, |\\___|_|         ${colors.green}|_|${colors.yellow}\\___/${colors.red}}|______|${colors.reset}
+            ${colors.blue}   _____                               ${colors.green} _______   ${colors.red} _${colors.reset}
+            ${colors.blue}  / ____|                              ${colors.green}|__   __|  ${colors.red}| |${colors.reset}
+            ${colors.blue} | (___   __ _ _ __   __ _  ___ _ __ ${colors.reset} ___ ${colors.green}| |${colors.yellow} ___ ${colors.red}| |${colors.reset}
+            ${colors.blue}  \\___ \\ / _` | '_ \\ / _` |/ _ \\ '__|${colors.reset}|___|${colors.green}| |${colors.yellow}/ _ \\${colors.red}| |${colors.reset}
+            ${colors.blue}  ____) | (_| | | | | (_| |  __/ |        ${colors.green}| |${colors.yellow} (_) ${colors.red}| |____${colors.reset}
+            ${colors.blue} |_____/ \\__,_|_| |_|\\__, |\\___|_|        ${colors.green}|_|${colors.yellow}\\___/${colors.red}|______|${colors.reset}
             ${colors.blue}                      __/ |${colors.reset}
             ${colors.blue}                     |___/${colors.reset}
             ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}

From d2e10453a95390ac1e788d001703f533217d1cf3 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:08:49 +0000
Subject: [PATCH 17/85] Support compressed files too

---
 bin/repeats_bed.py | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/bin/repeats_bed.py b/bin/repeats_bed.py
index 71043ea..d4ff4b7 100755
--- a/bin/repeats_bed.py
+++ b/bin/repeats_bed.py
@@ -2,6 +2,7 @@
 # This script was originally conceived by @muffato
 
 import argparse
+import gzip
 import sys
 
 __doc__ = "This script prints a BED file of the masked regions a fasta file."
@@ -10,7 +11,7 @@
 def fasta_to_bed(fasta):
 
     in_gap = None
-    with open(sys.argv[1]) as fh:
+    with gzip.open(fasta, "rt") if fasta.endswith(".gz") else open(fasta) as fh:
         for line in fh:
             line = line[:-1]
             if line.startswith(">"):
@@ -41,7 +42,7 @@ def fasta_to_bed(fasta):
 
     parser = argparse.ArgumentParser(description=__doc__)
     parser.add_argument("fasta", help="Input Fasta file.")
-    parser.add_argument("--version", action="version", version="%(prog)s 1.0")
+    parser.add_argument("--version", action="version", version="%(prog)s 1.1")
     args = parser.parse_args()
 
     fasta_to_bed(args.fasta)

From 8ac8ff2ec29b01b7c9a9f37f2bc79cc08de4174f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:09:42 +0000
Subject: [PATCH 18/85] Imported the latest shared subworkflows

---
 conf/base.config                           |  6 +--
 subworkflows/sanger-tol/prepare_fasta.nf   | 47 +++++++++++++++++++---
 subworkflows/sanger-tol/prepare_repeats.nf | 21 ++++++++--
 workflows/insdcdownload.nf                 |  2 +-
 4 files changed, 64 insertions(+), 12 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index aa29932..3a47a90 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -16,10 +16,10 @@ process {
     memory = { check_max( 50.MB * task.attempt, 'memory' ) }
     time   = { check_max( 30.min * task.attempt, 'time' ) }
 
-    // samtools dict takes more memory on larger genomes
+    // samtools dict loads entire sequences in memory
     withName: 'SAMTOOLS_DICT' {
-        // 50 MB per 500 Mbp
-        memory = { check_max( 50.MB + 50.MB * task.attempt * Math.ceil(fasta.size() / 500000000), 'memory' ) }
+        // 50 MB per 50 Mbp
+        memory = { check_max( 50.MB + 50.MB * task.attempt * Math.ceil(meta.max_length / 50000000), 'memory' ) }
     }
 
     withName:CUSTOM_DUMPSOFTWAREVERSIONS {
diff --git a/subworkflows/sanger-tol/prepare_fasta.nf b/subworkflows/sanger-tol/prepare_fasta.nf
index c2c576d..13b6859 100644
--- a/subworkflows/sanger-tol/prepare_fasta.nf
+++ b/subworkflows/sanger-tol/prepare_fasta.nf
@@ -24,16 +24,53 @@ workflow PREPARE_FASTA {
     ch_samtools_faidx   = CUSTOM_GETCHROMSIZES (ch_compressed_fasta).fai
     ch_versions         = ch_versions.mix(CUSTOM_GETCHROMSIZES.out.versions)
 
+    // Read the .fai file, extract sequence statistics, and make an extended meta map
+    sequence_map        = ch_samtools_faidx.map {
+        meta, fai -> [meta, meta + get_sequence_map(fai)]
+    }
+    // Update all channels to use the extended meta map
+    fasta_gz            = ch_compressed_fasta.join(sequence_map).map { [it[2], it[1]]}
+    faidx               = ch_samtools_faidx.join(sequence_map).map { [it[2], it[1]]}
+    gzi                 = CUSTOM_GETCHROMSIZES.out.gzi.join(sequence_map).map { [it[2], it[1]]}
+    sizes               = CUSTOM_GETCHROMSIZES.out.sizes.join(sequence_map).map { [it[2], it[1]]}
+    expanded_fasta      = fasta.join(sequence_map).map { [it[2], it[1]]}
+
     // Generate Samtools dictionary
-    ch_samtools_dict    = SAMTOOLS_DICT (fasta).dict
+    ch_samtools_dict    = SAMTOOLS_DICT (expanded_fasta).dict
     ch_versions         = ch_versions.mix(SAMTOOLS_DICT.out.versions)
 
 
     emit:
-    fasta_gz = ch_compressed_fasta       // path: genome.fa.gz
-    faidx    = ch_samtools_faidx         // path: genome.fa.gz.fai
+    fasta_gz = fasta_gz                  // path: genome.fa.gz
+    faidx    = faidx                     // path: genome.fa.gz.fai
     dict     = ch_samtools_dict          // path: genome.fa.dict
-    gzi      = CUSTOM_GETCHROMSIZES.out.gzi     // path: genome.fa.gz.gzi
-    sizes    = CUSTOM_GETCHROMSIZES.out.sizes   // path: genome.fa.gz.sizes
+    gzi      = gzi                       // path: genome.fa.gz.gzi
+    sizes    = sizes                     // path: genome.fa.gz.sizes
     versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ]
 }
+
+// Read the .fai file to extract the number of sequences, the maximum and total sequence length
+// Inspired from https://github.com/nf-core/rnaseq/blob/3.10.1/lib/WorkflowRnaseq.groovy
+def get_sequence_map(fai_file) {
+    def n_sequences = 0
+    def max_length = 0
+    def total_length = 0
+    fai_file.eachLine { line ->
+        def lspl   = line.split('\t')
+        def chrom  = lspl[0]
+        def length = lspl[1].toInteger()
+        n_sequences ++
+        total_length += length
+        if (length > max_length) {
+            max_length = length
+        }
+    }
+
+    def sequence_map = [:]
+    sequence_map.n_sequences = n_sequences
+    sequence_map.total_length = total_length
+    if (n_sequences) {
+        sequence_map.max_length = max_length
+    }
+    return sequence_map
+}
diff --git a/subworkflows/sanger-tol/prepare_repeats.nf b/subworkflows/sanger-tol/prepare_repeats.nf
index 7b25e03..64d23f1 100644
--- a/subworkflows/sanger-tol/prepare_repeats.nf
+++ b/subworkflows/sanger-tol/prepare_repeats.nf
@@ -26,10 +26,23 @@ workflow PREPARE_REPEATS {
     ch_compressed_bed   = TABIX_BGZIP ( ch_bed ).output
     ch_versions         = ch_versions.mix(TABIX_BGZIP.out.versions)
 
-    // Index the BED file in two formats for maximum compatibility
-    ch_indexed_bed_csi  = TABIX_TABIX_CSI ( ch_compressed_bed ).csi
+    // Try indexing the BED file in two formats for maximum compatibility
+    // but each has its own limitations
+    tabix_selector      = ch_compressed_bed.branch { meta, bed ->
+        tbi_and_csi: meta.max_length < 2**29
+        only_csi:    meta.max_length < 2**32
+        no_tabix:    true
+    }
+
+    // Output channels to tell the downstream subworkflows which indexes are missing
+    // (therefore, only meta is available)
+    no_csi              = tabix_selector.no_tabix.map {it[0]}
+    no_tbi              = tabix_selector.only_csi.mix(tabix_selector.no_tabix).map {it[0]}
+
+    // Do the indexing on the compatible Fasta files
+    ch_indexed_bed_csi  = TABIX_TABIX_CSI ( tabix_selector.tbi_and_csi.mix(tabix_selector.only_csi) ).csi
     ch_versions         = ch_versions.mix(TABIX_TABIX_CSI.out.versions)
-    ch_indexed_bed_tbi  = TABIX_TABIX_TBI ( ch_compressed_bed ).tbi
+    ch_indexed_bed_tbi  = TABIX_TABIX_TBI ( tabix_selector.tbi_and_csi ).tbi
     ch_versions         = ch_versions.mix(TABIX_TABIX_TBI.out.versions)
 
 
@@ -37,5 +50,7 @@ workflow PREPARE_REPEATS {
     bed_gz   = ch_compressed_bed            // path: genome.bed.gz
     bed_csi  = ch_indexed_bed_csi           // path: genome.bed.gz.csi
     bed_tbi  = ch_indexed_bed_tbi           // path: genome.bed.gz.tbi
+    no_csi   = no_csi                       // (only meta)
+    no_tbi   = no_tbi                       // (only meta)
     versions = ch_versions.ifEmpty(null)    // channel: [ versions.yml ]
 }
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index cfcb5cf..7308ce7 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -73,7 +73,7 @@ workflow INSDCDOWNLOAD {
     )
     ch_versions         = ch_versions.mix(PREPARE_REPEAT_MASKED_FASTA.out.versions)
     PREPARE_REPEATS (
-        DOWNLOAD_GENOME.out.fasta_masked
+        PREPARE_REPEAT_MASKED_FASTA.out.fasta_gz
     )
     ch_versions         = ch_versions.mix(PREPARE_REPEATS.out.versions)
 

From 0f538f951bde818e1846413ef1b0842f55beb335 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:10:41 +0000
Subject: [PATCH 19/85] Reordering of parameters to match the other pipelines
 and connect relative paths from the samplesheet to the --outdir parameter

---
 subworkflows/local/params_check.nf | 20 +++++++-------------
 workflows/insdcdownload.nf         | 14 ++++++++------
 2 files changed, 15 insertions(+), 19 deletions(-)

diff --git a/subworkflows/local/params_check.nf b/subworkflows/local/params_check.nf
index 3b7610b..8d4e31f 100644
--- a/subworkflows/local/params_check.nf
+++ b/subworkflows/local/params_check.nf
@@ -7,13 +7,13 @@ include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check'
 workflow PARAMS_CHECK {
 
     take:
-    inputs          // tuple, see below
+    samplesheet  // file
+    cli_params   // tuple, see below
+    outdir       // file output directory
 
 
     main:
 
-    def (samplesheet, assembly_accession, assembly_name, outdir) = inputs
-
     ch_versions = Channel.empty()
 
     ch_inputs = Channel.empty()
@@ -27,22 +27,16 @@ workflow PARAMS_CHECK {
             .map { [
                 it["assembly_accession"],
                 it["assembly_name"],
-                it["species_dir"],
+                it["species_dir"].startsWith("/") ? "" : outdir + "/",
             ] }
             .set { ch_inputs }
 
         ch_versions = ch_versions.mix(SAMPLESHEET_CHECK.out.versions)
 
     } else {
-
-        ch_inputs = Channel.of(
-            [
-                assembly_accession,
-                assembly_name,
-                outdir,
-            ]
-        )
-
+        // Add the other input channel in, as it's expected to have all the parameters in the right order
+        // except the output directory which must be appended
+        ch_inputs = ch_inputs.mix(cli_params.map { it + [outdir] } )
     }
 
 
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index 7308ce7..ac449b5 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -46,12 +46,14 @@ workflow INSDCDOWNLOAD {
     ch_versions = Channel.empty()
 
     PARAMS_CHECK (
-        [
-            params.input,
-            params.assembly_accession,
-            params.assembly_name,
-            params.outdir,
-        ]
+        params.input,
+        Channel.of(
+            [
+                params.assembly_accession,
+                params.assembly_name,
+            ]
+        ),
+        params.outdir,
     )
     ch_versions         = ch_versions.mix(PARAMS_CHECK.out.versions)
 

From b0212c4d8b44790e13836ab1e0ae52545e04111f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:11:52 +0000
Subject: [PATCH 20/85] Exclude the gene annotation files from md5checksums.txt

---
 modules/local/ncbi_download.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/modules/local/ncbi_download.nf b/modules/local/ncbi_download.nf
index 829e3bc..dc57a21 100644
--- a/modules/local/ncbi_download.nf
+++ b/modules/local/ncbi_download.nf
@@ -52,7 +52,8 @@ process NCBI_DOWNLOAD {
     wget ${ftp_path}/${remote_filename_stem}_genomic.fna.gz
     wget ${ftp_path}/md5checksums.txt
 
-    grep "\\(_assembly_report\\.txt\$\\|_assembly_stats\\.txt\$\\|_genomic\\.fna\\.gz\$\\)" md5checksums.txt > md5checksums_restricted.txt
+    grep "\\(_assembly_report\\.txt\$\\|_assembly_stats\\.txt\$\\|_genomic\\.fna\\.gz\$\\)" md5checksums.txt \
+        | grep -v "\\(_from_genomic\\.fna\\.gz\$\\)"> md5checksums_restricted.txt
     md5sum -c md5checksums_restricted.txt
     mv ${remote_filename_stem}_assembly_report.txt ${filename_assembly_report}
     mv ${remote_filename_stem}_assembly_stats.txt  ${filename_assembly_stats}

From 1c292c4fdcdd77e7dbaf857c4059cdaf34537538 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 19 Jan 2023 17:12:05 +0000
Subject: [PATCH 21/85] Provide a default value for --outdir

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 93c7650..92ff15b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,7 @@ params {
     ftp_root                   = "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA"
 
     // Boilerplate options
-    outdir                     = null
+    outdir                     = 'results'
     tracedir                   = "${params.outdir}/pipeline_info"
     publish_dir_mode           = 'copy'
     email                      = null

From 51437fd17df1acbb3cdd0a98b64561c6df5e202c Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 13:11:34 +0000
Subject: [PATCH 22/85] [prettier]

---
 .github/workflows/sangerfulltest.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index e89f0dd..9b68e67 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -11,7 +11,6 @@ jobs:
     name: Run LSF full size tests
     runs-on: ubuntu-latest
     steps:
-
       - name: Sets env vars for push
         run: |
           echo "REVISION=${{ github.sha }}" >> $GITHUB_ENV

From 9d4c07d04244a85c3d4c25d09a44acba75cd0380 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 13:15:07 +0000
Subject: [PATCH 23/85] nf-core lint fixes

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 093639e..4c29df8 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -12,6 +12,7 @@ lint:
     - .github/workflows/awsfulltest.yml
   files_unchanged:
     - LICENSE
+    - .gitattributes
     - .github/CONTRIBUTING.md
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - .github/workflows/linting.yml

From 5d2f67edf43f07b3119f459db2367e93f3752952 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 13:34:53 +0000
Subject: [PATCH 24/85] Pin nf-core to 2.5 since that is the version of the
 template I used

---
 .github/workflows/linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 8a5ce69..3a91d8e 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -82,7 +82,7 @@ jobs:
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core
+          pip install nf-core==2.5.0
 
       - name: Run nf-core lint
         env:

From cf19126e9f1ba4a429859cf6b88b3744c1895c0a Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 16:45:33 +0000
Subject: [PATCH 25/85] --outdir is now mandatory

---
 lib/WorkflowInsdcdownload.groovy | 8 ++++++--
 1 file changed, 6 insertions(+), 2 deletions(-)

diff --git a/lib/WorkflowInsdcdownload.groovy b/lib/WorkflowInsdcdownload.groovy
index 81b679d..893d8b8 100755
--- a/lib/WorkflowInsdcdownload.groovy
+++ b/lib/WorkflowInsdcdownload.groovy
@@ -17,11 +17,15 @@ class WorkflowInsdcdownload {
                 System.exit(1)
             }
         } else {
-            if (!params.assembly_accession || !params.assembly_name || !params.outdir) {
-                log.error "Either --input, or --assembly_accession, --assembly_name, and --outdir must be provided"
+            if (!params.assembly_accession || !params.assembly_name) {
+                log.error "Either --input, or --assembly_accession and --assembly_name must be provided"
                 System.exit(1)
             }
         }
+        if (!params.outdir) {
+            log.error "--outdir is mandatory"
+            System.exit(1)
+        }
     }
 
 }

From 9bb76be43d621a13058ae7f13ab5221ca43a9c62 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 18:36:49 +0000
Subject: [PATCH 26/85] Updated the change-log

---
 CHANGELOG.md | 12 ++++++++++++
 1 file changed, 12 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7b386e7..b518284 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,18 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## v1.2.0 - [date]
+
+### `Fixed`
+
+- Relative paths in the sample-sheet are now evaluated from the `--outdir` parameter
+- Memory usage rules for `samtools dict`
+- Appropriate use of `tabix`'s TBI and CSI indexing, depending on the sequence lengths
+
+### `Added`
+
+- `--outdir` is a _mandatory_ parameter
+
 ## v1.1.0 - [2022-10-07]
 
 Minor update that fixes a few bugs

From d2b5de94cf3e90c50b841745b5a912c2895fd9c1 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 20 Jan 2023 18:37:01 +0000
Subject: [PATCH 27/85] Doc update

---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index d5ccd56..2820121 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -70,7 +70,7 @@ the directory structure includes the assembly name, e.g. `gfLaeSulp1.1`, and all
 - `GCA_*.masked.ncbi.fa.gz`: Masked assembly in Fasta format, compressed with `bgzip` (whose index is `GCA_*.fa.gz.gzi`)
 - `GCA_*.masked.ncbi.fa.gz.fai`: `samtools faidx` index, which allows accessing any region of the assembly in constant time
 - `GCA_*.masked.ncbi.fa.dict`: `samtools dict` index, which allows identifying a sequence by its MD5 checksum
-- `GCA_*.masked.ncbi.bed.gz`: BED file with the coordinates of the regions masked by the NCBI pipeline, with accompanying `tabix` indices (`.csi` and `.tbi`)
+- `GCA_*.masked.ncbi.bed.gz`: BED file with the coordinates of the regions masked by the NCBI pipeline, with accompanying `tabix` indices (`.csi` and `.tbi`), depending on the sequence lengths
 
 ### Pipeline information
 

From 631688ce4d9481c768c93e0246aed5b935851ec8 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 23 Jan 2023 10:23:13 +0000
Subject: [PATCH 28/85] bugfix: was discarding species_dir

---
 subworkflows/local/params_check.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/params_check.nf b/subworkflows/local/params_check.nf
index 8d4e31f..8992365 100644
--- a/subworkflows/local/params_check.nf
+++ b/subworkflows/local/params_check.nf
@@ -27,7 +27,7 @@ workflow PARAMS_CHECK {
             .map { [
                 it["assembly_accession"],
                 it["assembly_name"],
-                it["species_dir"].startsWith("/") ? "" : outdir + "/",
+                (it["species_dir"].startsWith("/") ? "" : outdir + "/") + it["species_dir"],
             ] }
             .set { ch_inputs }
 

From 8ab4b3a15bfe8af2ca9c9d89148d08b33aaf92aa Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Wed, 1 Feb 2023 08:11:20 +0000
Subject: [PATCH 29/85] Optimised resource usage for tabix too

---
 conf/base.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/conf/base.config b/conf/base.config
index 3a47a90..c3863bb 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -16,6 +16,12 @@ process {
     memory = { check_max( 50.MB * task.attempt, 'memory' ) }
     time   = { check_max( 30.min * task.attempt, 'time' ) }
 
+    // tabix needs pointers to the sequences in memory
+    withName: '.*:.*:PREPARE_REPEATS:TABIX_TABIX_.*' {
+        // 50 MB per 25,000 sequences
+        memory = { check_max( 50.MB * task.attempt * Math.ceil(meta.n_sequences / 25000), 'memory' ) }
+    }
+
     // samtools dict loads entire sequences in memory
     withName: 'SAMTOOLS_DICT' {
         // 50 MB per 50 Mbp

From fedd540dea03f5c65f0dbf1084670b626c96df99 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Wed, 1 Feb 2023 08:19:26 +0000
Subject: [PATCH 30/85] More information about the output files

---
 docs/output.md |  3 ++-
 docs/usage.md  | 12 ++++++++----
 2 files changed, 10 insertions(+), 5 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 2820121..ab56998 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -4,7 +4,8 @@
 
 This document describes the output produced by the pipeline.
 
-The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory.
+The directories listed below will be created in the results directory (or `species_dir` when using a samplesheet) after the pipeline has finished.
+All paths are relative to the top-level results directory.
 
 The directories comply with Tree of Life's canonical directory structure.
 
diff --git a/docs/usage.md b/docs/usage.md
index 0217a00..5499571 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -37,18 +37,22 @@ nextflow run sanger-tol/insdcdownload --input '[path to samplesheet file]' --out
 The values in the file must correspond to the values you would add to the `--assembly_accession` and `--assembly_name` parameters.
 
 ```console
-assembly_accession,assembly_name
-GCA_927399515.1,gfLaeSulp1.1
-GCA_922984935.2,mMelMel3.2_paternal_haplotype
+species_dir,assembly_name,assembly_accession
+darwin/data/fungi/Laetiporus_sulphureus,gfLaeSulp1.1,GCA_927399515.1
+darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
 ```
 
 | Column               | Description                                                                    |
 | -------------------- | ------------------------------------------------------------------------------ |
-| `assembly_accession` | Accession number of the assembly to download. Typically of the form `GCA_*.*`. |
+| `species_dir`        | Base download directory for this species. Evaluated from `--outdir` if relative. |
 | `assembly_name`      | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.             |
+| `assembly_accession` | Accession number of the assembly to download. Typically of the form `GCA_*.*`. |
 
 An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
 
+`--species_dir` is used to fit the output of this pipeline into a directory structure compatible with the other pipelines
+from Sanger Tree of Life.
+
 ### Updating the pipeline
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:

From 1e82b2bba11a35a6b5ff86ade6269bf9196b01ff Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Wed, 1 Feb 2023 10:53:10 +0000
Subject: [PATCH 31/85] Nextflow version bump due to the way Conda is
 enabled/disabled

---
 .github/workflows/ci.yml | 2 +-
 README.md                | 4 ++--
 nextflow.config          | 2 +-
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 8327968..34d0a5f 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       matrix:
         NXF_VER:
-          - "22.04.0"
+          - "22.10.0"
           - "latest-everything"
     steps:
       - name: Check out pipeline code
diff --git a/README.md b/README.md
index f3d6b5d..c4ec659 100644
--- a/README.md
+++ b/README.md
@@ -6,7 +6,7 @@
 
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7155119-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7155119)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.04.0-23aa62.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.0-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -43,7 +43,7 @@ Steps involved:
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.04.0`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.0`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
 
diff --git a/nextflow.config b/nextflow.config
index 92ff15b..949b90d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -172,7 +172,7 @@ manifest {
     homePage        = 'https://github.com/sanger-tol/insdcdownload'
     description     = 'Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure'
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=22.04.0'
+    nextflowVersion = '!>=22.10.0'
     version         = '1.2dev'
 }
 

From fc4a7894e950ea094565f5de3d6e238ba7b0d2f3 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Wed, 1 Feb 2023 10:55:42 +0000
Subject: [PATCH 32/85] Documentation update

---
 docs/usage.md | 32 ++++++++++++++++++++------------
 1 file changed, 20 insertions(+), 12 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 5499571..aafdd43 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -5,9 +5,13 @@
 The pipeline takes an assembly accession number, as well as the assembly name, and downloads it in a given directory.
 It also extracts the repeat-masking performed by the NCBI, and builds a set of common indices (such as `samtools faidx`).
 
-## Running the pipeline
+## One-off downloads
 
-The typical command for running the pipeline is as follows:
+The pipeline accepts command-one line arguments to specify a single genome to download:
+
+- `--assembly_name`: The name of the assembly,
+- `--assembly_accession`: The accession number of the assembly,
+- `--outdir`: Where to download the data.
 
 ```console
 nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir <OUTDIR>
@@ -16,19 +20,11 @@ nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --ass
 This will launch the pipeline and download the `gfLaeSulp1.1` assembly (accession `GCA_927399515.1`) into the `<OUTDIR>/` directory,
 which will be created if needed.
 
-Note that the pipeline will create the following files in your working directory:
-
-```bash
-work                # Directory containing the nextflow working files
-<OUTDIR>            # Finished results in specified location (defined with --outdir)
-.nextflow_log       # Log file from Nextflow
-# Other nextflow hidden files, eg. history of pipeline runs and old logs.
-```
 
 ## Bulk download
 
 The pipeline can download multiple assemblies at once, by providing them in a `.csv` file through the `--input` parameter.
-It has to be a comma-separated file with 2 columns, and a header row as shown in the examples below.
+It has to be a comma-separated file with three columns, and a header row as shown in the examples below.
 
 ```console
 nextflow run sanger-tol/insdcdownload --input '[path to samplesheet file]' --outdir <OUTDIR>
@@ -50,9 +46,21 @@ darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
 
 An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
 
-`--species_dir` is used to fit the output of this pipeline into a directory structure compatible with the other pipelines
+`species_dir` is used to fit the output of this pipeline into a directory structure compatible with the other pipelines
 from Sanger Tree of Life.
 
+## Nextflow outputs
+
+Note that the pipeline will create the following files in your working directory:
+
+```bash
+work                # Directory containing the nextflow working files
+<OUTDIR>            # Finished results in specified location (defined with --outdir)
+.nextflow_log       # Log file from Nextflow
+.nextflow           # Directory where Nextflow keeps track of jobs
+# Other nextflow hidden files, eg. history of pipeline runs and old logs.
+```
+
 ### Updating the pipeline
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:

From 3480e0ad52b5fc91942d17f26d1addc2b811196c Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Wed, 1 Feb 2023 11:23:27 +0000
Subject: [PATCH 33/85] Documentation update

---
 .nf-core.yml       |  1 +
 docs/README.md     |  2 ++
 docs/output.md     |  1 +
 docs/parameters.md | 67 ++++++++++++++++++++++++++++++++++++++++++++++
 docs/usage.md      | 33 +++++++++++++----------
 5 files changed, 90 insertions(+), 14 deletions(-)
 create mode 100644 docs/parameters.md

diff --git a/.nf-core.yml b/.nf-core.yml
index 4c29df8..40826dc 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -17,6 +17,7 @@ lint:
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - .github/workflows/linting.yml
     - assets/sendmail_template.txt
+    - docs/README.md
     - lib/NfcoreTemplate.groovy
   nextflow_config:
     - manifest.name
diff --git a/docs/README.md b/docs/README.md
index 60d64f6..7b74c4f 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -4,5 +4,7 @@ The sanger-tol/insdcdownload documentation is split into the following pages:
 
 - [Usage](usage.md)
   - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
+- [Parameters](parameters.md)
+  - An overview of the different pipeline options and how to use them.
 - [Output](output.md)
   - An overview of the different results produced by the pipeline and how to interpret them.
diff --git a/docs/output.md b/docs/output.md
index ab56998..30b1452 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -78,5 +78,6 @@ the directory structure includes the assembly name, e.g. `gfLaeSulp1.1`, and all
 - `pipeline_info/`
   - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
   - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
+  - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
 
 [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
diff --git a/docs/parameters.md b/docs/parameters.md
new file mode 100644
index 0000000..a4d1569
--- /dev/null
+++ b/docs/parameters.md
@@ -0,0 +1,67 @@
+# sanger-tol/insdcdownload pipeline parameters
+
+Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure
+
+## Input/output options
+
+Define where the pipeline should find input data and save output data.
+
+| Parameter                                                                                                                                                        | Description                                                                                                                                                                                          | Type     | Default | Required | Hidden |
+| ---------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ |
+| `assembly_accession`                                                                                                                                             | Accession number of the assembly to download. Typically of the form `GCA_*.*`                                                                                                                        | `string` |         |          |        |
+| `assembly_name`                                                                                                                                                  | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.                                                                                                                                   | `string` |         |          |        |
+| `outdir`                                                                                                                                                         | The output directory where the results will be saved. Not considered when running the pipeline with a .csv file as input.                                                                            | `string` |         |          |        |
+| `input`                                                                                                                                                          | Path to comma-separated file containing information about the assemblies to download. Used for bulk download of many assemblies. <details><summary>Help</summary><small>The file has to be a         |
+| comma-separated file with three columns, and a header row. The columns names must be `species_dir`, `assembly_accession`, and `assembly_name`.</small></details> | `string`                                                                                                                                                                                             |          |         |          |
+| `ftp_root`                                                                                                                                                       | Root location of the NCBI FTP, in which all assemblies can be found. Access protocol is actually not limited to FTP, and we use HTTPS by default.                                                    | `string` |
+| https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA                                                                                                                     |                                                                                                                                                                                                      | True     |
+| `email`                                                                                                                                                          | Email address for completion summary. <details><summary>Help</summary><small>Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow |
+| exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.</small></details>           | `string`                                                                                                                                                                                             |          |         | True     |
+
+## Institutional config options
+
+Parameters used to describe centralised config profiles. These should not be edited.
+
+| Parameter                                                                                                                                                                                                  | Description                                                                                                                                                                            | Type     | Default | Required | Hidden |
+| ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ |
+| `custom_config_version`                                                                                                                                                                                    | Git commit id for Institutional configs.                                                                                                                                               | `string` | master  |          | True   |
+| `custom_config_base`                                                                                                                                                                                       | Base directory for Institutional configs. <details><summary>Help</summary><small>If you're running offline, Nextflow will not be able to fetch the institutional config files from the |
+| internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.</small></details> |
+| `string`                                                                                                                                                                                                   | https://raw.githubusercontent.com/nf-core/configs/master                                                                                                                               |          | True    |
+| `config_profile_name`                                                                                                                                                                                      | Institutional config name.                                                                                                                                                             | `string` |         |          | True   |
+| `config_profile_description`                                                                                                                                                                               | Institutional config description.                                                                                                                                                      | `string` |         |          | True   |
+| `config_profile_contact`                                                                                                                                                                                   | Institutional config contact information.                                                                                                                                              | `string` |         |          | True   |
+| `config_profile_url`                                                                                                                                                                                       | Institutional config URL link.                                                                                                                                                         | `string` |         |          | True   |
+
+## Max job request options
+
+Set the top limit for requested resources for any single job.
+
+| Parameter                                                                      | Description                                                                                                                                                                                        | Type   | Default | Required | Hidden |
+| ------------------------------------------------------------------------------ | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------ | ------- | -------- | ------ |
+| `max_cpus`                                                                     | Maximum number of CPUs that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the CPU requirement for each process. Should be an integer   |
+| e.g. `--max_cpus 1`</small></details>                                          | `integer`                                                                                                                                                                                          | 16     |         | True     |
+| `max_memory`                                                                   | Maximum amount of memory that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the memory requirement for each process. Should be a       |
+| string in the format integer-unit e.g. `--max_memory '8.GB'`</small></details> | `string`                                                                                                                                                                                           | 128.GB |         | True     |
+| `max_time`                                                                     | Maximum amount of time that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the time requirement for each process. Should be a string in |
+| the format integer-unit e.g. `--max_time '2.h'`</small></details>              | `string`                                                                                                                                                                                           | 240.h  |         | True     |
+
+## Generic options
+
+Less common options for the pipeline, typically set in a config file.
+
+| Parameter                                                                                                                                                                                                        | Description                                                                                                                                                                                   | Type      | Default                        | Required | Hidden |
+| ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------------------------------ | -------- | ------ |
+| `help`                                                                                                                                                                                                           | Display help text.                                                                                                                                                                            | `boolean` |                                |          | True   |
+| `publish_dir_mode`                                                                                                                                                                                               | Method used to save pipeline results to output directory. <details><summary>Help</summary><small>The Nextflow `publishDir` option specifies which intermediate files should be saved to the   |
+| output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.</small></details> |
+| `string`                                                                                                                                                                                                         | copy                                                                                                                                                                                          |           | True                           |
+| `email_on_fail`                                                                                                                                                                                                  | Email address for completion summary, only when pipeline fails. <details><summary>Help</summary><small>An email address to send a summary email to when the pipeline is completed - ONLY sent |
+| if the pipeline does not exit successfully.</small></details>                                                                                                                                                    | `string`                                                                                                                                                                                      |           |                                | True     |
+| `plaintext_email`                                                                                                                                                                                                | Send plain-text email instead of HTML.                                                                                                                                                        | `boolean` |                                |          | True   |
+| `monochrome_logs`                                                                                                                                                                                                | Do not use coloured log outputs.                                                                                                                                                              | `boolean` |                                |          | True   |
+| `tracedir`                                                                                                                                                                                                       | Directory to keep pipeline Nextflow logs and reports.                                                                                                                                         | `string`  | ${params.outdir}/pipeline_info |          | True   |
+| `validate_params`                                                                                                                                                                                                | Boolean whether to validate parameters against the schema at runtime                                                                                                                          | `boolean` | True                           |          | True   |
+| `show_hidden_params`                                                                                                                                                                                             | Show all params when using `--help` <details><summary>Help</summary><small>By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with       |
+| `--help`. Specifying this option will tell the pipeline to show all parameters.</small></details>                                                                                                                | `boolean`                                                                                                                                                                                     |           |                                | True     |
+| `enable_conda`                                                                                                                                                                                                   | Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.                                                                                          | `boolean` |                                |          | True   |
diff --git a/docs/usage.md b/docs/usage.md
index aafdd43..29caf4a 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -14,41 +14,46 @@ The pipeline accepts command-one line arguments to specify a single genome to do
 - `--outdir`: Where to download the data.
 
 ```console
-nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir <OUTDIR>
+nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir gfLaeSulp1.1_data
 ```
 
-This will launch the pipeline and download the `gfLaeSulp1.1` assembly (accession `GCA_927399515.1`) into the `<OUTDIR>/` directory,
+This will launch the pipeline and download the `gfLaeSulp1.1` assembly (accession `GCA_927399515.1`) into the `gfLaeSulp1.1_data/` directory,
 which will be created if needed.
 
-
 ## Bulk download
 
 The pipeline can download multiple assemblies at once, by providing them in a `.csv` file through the `--input` parameter.
 It has to be a comma-separated file with three columns, and a header row as shown in the examples below.
 
-```console
-nextflow run sanger-tol/insdcdownload --input '[path to samplesheet file]' --outdir <OUTDIR>
-```
-
-The values in the file must correspond to the values you would add to the `--assembly_accession` and `--assembly_name` parameters.
-
 ```console
 species_dir,assembly_name,assembly_accession
 darwin/data/fungi/Laetiporus_sulphureus,gfLaeSulp1.1,GCA_927399515.1
 darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
 ```
 
-| Column               | Description                                                                    |
-| -------------------- | ------------------------------------------------------------------------------ |
+| Column               | Description                                                                      |
+| -------------------- | -------------------------------------------------------------------------------- |
 | `species_dir`        | Base download directory for this species. Evaluated from `--outdir` if relative. |
-| `assembly_name`      | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.             |
-| `assembly_accession` | Accession number of the assembly to download. Typically of the form `GCA_*.*`. |
+| `assembly_name`      | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.               |
+| `assembly_accession` | Accession number of the assembly to download. Typically of the form `GCA_*.*`.   |
 
-An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
+A samplesheet may contain:
 
+- multiple assemblies of the same species
+- multiple assemblies in the same output directory
+- only one row per assembly
+
+All samplesheet columns correspond exactly to their corresponding command-line parameter,
+except `species_dir` which overrides or complements `--oudir`.
 `species_dir` is used to fit the output of this pipeline into a directory structure compatible with the other pipelines
 from Sanger Tree of Life.
 
+An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
+
+```bash
+nextflow run sanger-tol/insdcdownload -profile singularity --input /path/to/samplesheet.csv --outdir /path/to/results
+```
+
 ## Nextflow outputs
 
 Note that the pipeline will create the following files in your working directory:

From cf7443fc46258c8ecaa372a701b01dc50351f924 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 13 Apr 2023 11:33:51 +0100
Subject: [PATCH 34/85] New cleanup profile to tidy up the integration runs on
 the farm

---
 nextflow.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/nextflow.config b/nextflow.config
index 949b90d..7fa7066 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -68,6 +68,7 @@ try {
 
 
 profiles {
+    cleanup { cleanup = true }
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
         params.enable_conda    = true

From 0a9c6486241ef4633b4c9bd9af751af8d7214c48 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 09:56:38 +0100
Subject: [PATCH 35/85] Template update for nf-core/tools version 2.6

---
 .prettierignore                               |  1 +
 CITATION.cff                                  |  8 +--
 assets/adaptivecard.json                      | 67 +++++++++++++++++++
 assets/methods_description_template.yml       | 25 +++++++
 assets/multiqc_config.yml                     |  6 +-
 bin/check_samplesheet.py                      |  9 ++-
 docs/usage.md                                 |  8 +++
 lib/NfcoreTemplate.groovy                     | 55 +++++++++++++++
 lib/Utils.groovy                              | 21 ++++--
 lib/WorkflowInsdcdownload.groovy              | 19 ++++++
 modules.json                                  | 27 ++++----
 .../custom/dumpsoftwareversions/main.nf       |  8 +--
 .../custom/dumpsoftwareversions/meta.yml      |  0
 .../templates/dumpsoftwareversions.py         |  0
 modules/nf-core/{modules => }/fastqc/main.nf  | 12 ++++
 modules/nf-core/{modules => }/fastqc/meta.yml |  0
 modules/nf-core/modules/multiqc/main.nf       | 31 ---------
 modules/nf-core/multiqc/main.nf               | 53 +++++++++++++++
 .../nf-core/{modules => }/multiqc/meta.yml    | 15 +++++
 nextflow.config                               |  5 +-
 nextflow_schema.json                          | 18 +++++
 pyproject.toml                                | 10 +++
 workflows/insdcdownload.nf                    | 26 ++++---
 23 files changed, 351 insertions(+), 73 deletions(-)
 create mode 100644 assets/adaptivecard.json
 create mode 100644 assets/methods_description_template.yml
 mode change 100755 => 100644 lib/Utils.groovy
 rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/main.nf (79%)
 rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/meta.yml (100%)
 rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py (100%)
 rename modules/nf-core/{modules => }/fastqc/main.nf (85%)
 rename modules/nf-core/{modules => }/fastqc/meta.yml (100%)
 delete mode 100644 modules/nf-core/modules/multiqc/main.nf
 create mode 100644 modules/nf-core/multiqc/main.nf
 rename modules/nf-core/{modules => }/multiqc/meta.yml (73%)
 create mode 100644 pyproject.toml

diff --git a/.prettierignore b/.prettierignore
index d0e7ae5..eb74a57 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -1,4 +1,5 @@
 email_template.html
+adaptivecard.json
 .nextflow*
 work/
 data/
diff --git a/CITATION.cff b/CITATION.cff
index 4533e2f..017666c 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -13,8 +13,8 @@ authors:
     given-names: Johannes
   - family-names: Wilm
     given-names: Andreas
-  - family-names: Ulysse Garcia
-    given-names: Maxime
+  - family-names: Garcia
+    given-names: Maxime Ulysse
   - family-names: Di Tommaso
     given-names: Paolo
   - family-names: Nahnsen
@@ -39,8 +39,8 @@ prefered-citation:
       given-names: Johannes
     - family-names: Wilm
       given-names: Andreas
-    - family-names: Ulysse Garcia
-      given-names: Maxime
+    - family-names: Garcia
+      given-names: Maxime Ulysse
     - family-names: Di Tommaso
       given-names: Paolo
     - family-names: Nahnsen
diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json
new file mode 100644
index 0000000..44934f8
--- /dev/null
+++ b/assets/adaptivecard.json
@@ -0,0 +1,67 @@
+{
+    "type": "message",
+    "attachments": [
+        {
+            "contentType": "application/vnd.microsoft.card.adaptive",
+            "contentUrl": null,
+            "content": {
+                "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json",
+                "msteams": {
+                    "width": "Full"
+                },
+                "type": "AdaptiveCard",
+                "version": "1.2",
+                "body": [
+                    {
+                        "type": "TextBlock",
+                        "size": "Large",
+                        "weight": "Bolder",
+                        "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>",
+                        "text": "sanger-tol/insdcdownload v${version} - ${runName}",
+                        "wrap": true
+                    },
+                    {
+                        "type": "TextBlock",
+                        "spacing": "None",
+                        "text": "Completed at ${dateComplete} (duration: ${duration})",
+                        "isSubtle": true,
+                        "wrap": true
+                    },
+                    {
+                        "type": "TextBlock",
+                        "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>",
+                        "wrap": true
+                    },
+                    {
+                        "type": "TextBlock",
+                        "text": "The command used to launch the workflow was as follows:",
+                        "wrap": true
+                    },
+                    {
+                        "type": "TextBlock",
+                        "text": "${commandLine}",
+                        "isSubtle": true,
+                        "wrap": true
+                    }
+                ],
+                "actions": [
+                    {
+                        "type": "Action.ShowCard",
+                        "title": "Pipeline Configuration",
+                        "card": {
+                            "type": "AdaptiveCard",
+                            "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json",
+                            "body": [
+                                {
+                                    "type": "FactSet",
+                                    "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %>
+                                    ]
+                                }
+                            ]
+                        }
+                    }
+                ]
+            }
+        }
+    ]
+}
diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml
new file mode 100644
index 0000000..76b0c0e
--- /dev/null
+++ b/assets/methods_description_template.yml
@@ -0,0 +1,25 @@
+id: "sanger-tol-insdcdownload-methods-description"
+description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication."
+section_name: "sanger-tol/insdcdownload Methods Description"
+section_href: "https://github.com/sanger-tol/insdcdownload"
+plot_type: "html"
+## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline
+## You inject any metadata in the Nextflow '${workflow}' object
+data: |
+  <h4>Methods</h4>
+  <p>Data was processed using sanger-tol/insdcdownload v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>).</p>
+  <p>The pipeline was executed with Nextflow v${workflow.nextflow.version} (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p>
+  <pre><code>${workflow.commandLine}</code></pre>
+  <h4>References</h4>
+  <ul>
+    <li>Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. <a href="https://doi.org/10.1038/nbt.3820">https://doi.org/10.1038/nbt.3820</a></li>
+    <li>Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. <a href="https://doi.org/10.1038/s41587-020-0439-x">https://doi.org/10.1038/s41587-020-0439-x</a></li>
+  </ul>
+  <div class="alert alert-info">
+    <h5>Notes:</h5>
+    <ul>
+      ${nodoi_text}
+      <li>The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!</li>
+      <li>You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.</li>
+    </ul>
+  </div>
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 407fee5..0860151 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -2,9 +2,11 @@ report_comment: >
   This report has been generated by the <a href="https://github.com/sanger-tol/insdcdownload" target="_blank">sanger-tol/insdcdownload</a>
   analysis pipeline.
 report_section_order:
-  software_versions:
+  "sanger-tol-insdcdownload-methods-description":
     order: -1000
-  "sanger-tol-insdcdownload-summary":
+  software_versions:
     order: -1001
+  "sanger-tol-insdcdownload-summary":
+    order: -1002
 
 export_plots: true
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 9a8b896..11b1557 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -98,7 +98,9 @@ def _validate_pair(self, row):
         """Assert that read pairs have the same file extension. Report pair status."""
         if row[self._first_col] and row[self._second_col]:
             row[self._single_col] = False
-            if Path(row[self._first_col]).suffixes[-2:] != Path(row[self._second_col]).suffixes[-2:]:
+            first_col_suffix = Path(row[self._first_col]).suffixes[-2:]
+            second_col_suffix = Path(row[self._second_col]).suffixes[-2:]
+            if first_col_suffix != second_col_suffix:
                 raise AssertionError("FASTQ pairs must have the same file extensions.")
         else:
             row[self._single_col] = True
@@ -157,7 +159,7 @@ def sniff_format(handle):
     handle.seek(0)
     sniffer = csv.Sniffer()
     if not sniffer.has_header(peek):
-        logger.critical(f"The given sample sheet does not appear to contain a header.")
+        logger.critical("The given sample sheet does not appear to contain a header.")
         sys.exit(1)
     dialect = sniffer.sniff(peek)
     return dialect
@@ -195,7 +197,8 @@ def check_samplesheet(file_in, file_out):
         reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle))
         # Validate the existence of the expected header columns.
         if not required_columns.issubset(reader.fieldnames):
-            logger.critical(f"The sample sheet **must** contain the column headers: {', '.join(required_columns)}.")
+            req_cols = ", ".join(required_columns)
+            logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.")
             sys.exit(1)
         # Validate each row.
         checker = RowChecker()
diff --git a/docs/usage.md b/docs/usage.md
index ce53d51..58365c3 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -235,6 +235,14 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config
 
 If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
 
+## Azure Resource Requests
+
+To be used with the `azurebatch` profile by specifying the `-profile azurebatch`.
+We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required.
+
+Note that the choice of VM size depends on your quota and the overall workload during the analysis.
+For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes).
+
 ## Running in the background
 
 Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 8f85736..45992fa 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -145,6 +145,61 @@ class NfcoreTemplate {
         output_tf.withWriter { w -> w << email_txt }
     }
 
+    //
+    // Construct and send adaptive card
+    // https://adaptivecards.io
+    //
+    public static void adaptivecard(workflow, params, summary_params, projectDir, log) {
+        def hook_url = params.hook_url
+
+        def summary = [:]
+        for (group in summary_params.keySet()) {
+            summary << summary_params[group]
+        }
+
+        def misc_fields = [:]
+        misc_fields['start']                                = workflow.start
+        misc_fields['complete']                             = workflow.complete
+        misc_fields['scriptfile']                           = workflow.scriptFile
+        misc_fields['scriptid']                             = workflow.scriptId
+        if (workflow.repository) misc_fields['repository']  = workflow.repository
+        if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
+        if (workflow.revision)   misc_fields['revision']    = workflow.revision
+        misc_fields['nxf_version']                          = workflow.nextflow.version
+        misc_fields['nxf_build']                            = workflow.nextflow.build
+        misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
+
+        def msg_fields = [:]
+        msg_fields['version']      = workflow.manifest.version
+        msg_fields['runName']      = workflow.runName
+        msg_fields['success']      = workflow.success
+        msg_fields['dateComplete'] = workflow.complete
+        msg_fields['duration']     = workflow.duration
+        msg_fields['exitStatus']   = workflow.exitStatus
+        msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
+        msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
+        msg_fields['commandLine']  = workflow.commandLine
+        msg_fields['projectDir']   = workflow.projectDir
+        msg_fields['summary']      = summary << misc_fields
+
+        // Render the JSON template
+        def engine       = new groovy.text.GStringTemplateEngine()
+        def hf = new File("$projectDir/assets/adaptivecard.json")
+        def json_template = engine.createTemplate(hf).make(msg_fields)
+        def json_message  = json_template.toString()
+
+        // POST
+        def post = new URL(hook_url).openConnection();
+        post.setRequestMethod("POST")
+        post.setDoOutput(true)
+        post.setRequestProperty("Content-Type", "application/json")
+        post.getOutputStream().write(json_message.getBytes("UTF-8"));
+        def postRC = post.getResponseCode();
+        if (! postRC.equals(200)) {
+            log.warn(post.getErrorStream().getText());
+        }
+    }
+
     //
     // Print pipeline summary on completion
     //
diff --git a/lib/Utils.groovy b/lib/Utils.groovy
old mode 100755
new mode 100644
index 28567bd..8d030f4
--- a/lib/Utils.groovy
+++ b/lib/Utils.groovy
@@ -21,19 +21,26 @@ class Utils {
         }
 
         // Check that all channels are present
-        def required_channels = ['conda-forge', 'bioconda', 'defaults']
-        def conda_check_failed = !required_channels.every { ch -> ch in channels }
+        // This channel list is ordered by required channel priority.
+        def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
+        def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
 
         // Check that they are in the right order
-        conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda'))
-        conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
+        def channel_priority_violation = false
+        def n = required_channels_in_order.size()
+        for (int i = 0; i < n - 1; i++) {
+            channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
+        }
 
-        if (conda_check_failed) {
+        if (channels_missing | channel_priority_violation) {
             log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
                 "  There is a problem with your Conda configuration!\n\n" +
                 "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-                "  Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
-                "  NB: The order of the channels matters!\n" +
+                "  Please refer to https://bioconda.github.io/\n" +
+                "  The observed channel order is \n" +
+                "  ${channels}\n" +
+                "  but the following channel order is required:\n" +
+                "  ${required_channels_in_order}\n" +
                 "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
         }
     }
diff --git a/lib/WorkflowInsdcdownload.groovy b/lib/WorkflowInsdcdownload.groovy
index 06ad804..d5d0f5d 100755
--- a/lib/WorkflowInsdcdownload.groovy
+++ b/lib/WorkflowInsdcdownload.groovy
@@ -2,6 +2,8 @@
 // This file holds several functions specific to the workflow/insdcdownload.nf in the sanger-tol/insdcdownload pipeline
 //
 
+import groovy.text.SimpleTemplateEngine
+
 class WorkflowInsdcdownload {
 
     //
@@ -41,4 +43,21 @@ class WorkflowInsdcdownload {
         yaml_file_text        += "data: |\n"
         yaml_file_text        += "${summary_section}"
         return yaml_file_text
+    }
+
+    public static String methodsDescriptionText(run_workflow, mqc_methods_yaml) {
+        // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
+        def meta = [:]
+        meta.workflow = run_workflow.toMap()
+        meta["manifest_map"] = run_workflow.manifest.toMap()
+
+        meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
+        meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+
+        def methods_text = mqc_methods_yaml.text
+
+        def engine =  new SimpleTemplateEngine()
+        def description_html = engine.createTemplate(methods_text).make(meta)
+
+        return description_html
     }}
diff --git a/modules.json b/modules.json
index 73457fd..3ae60e0 100644
--- a/modules.json
+++ b/modules.json
@@ -2,20 +2,21 @@
     "name": "sanger-tol/insdcdownload",
     "homePage": "https://github.com/sanger-tol/insdcdownload",
     "repos": {
-        "nf-core/modules": {
-            "git_url": "https://github.com/nf-core/modules.git",
+        "https://github.com/nf-core/modules.git": {
             "modules": {
-                "custom/dumpsoftwareversions": {
-                    "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d",
-                    "branch": "master"
-                },
-                "fastqc": {
-                    "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d",
-                    "branch": "master"
-                },
-                "multiqc": {
-                    "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d",
-                    "branch": "master"
+                "nf-core": {
+                    "custom/dumpsoftwareversions": {
+                        "branch": "master",
+                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                    },
+                    "fastqc": {
+                        "branch": "master",
+                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                    },
+                    "multiqc": {
+                        "branch": "master",
+                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                    }
                 }
             }
         }
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
similarity index 79%
rename from modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
rename to modules/nf-core/custom/dumpsoftwareversions/main.nf
index 327d510..cebb6e0 100644
--- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -1,11 +1,11 @@
 process CUSTOM_DUMPSOFTWAREVERSIONS {
-    label 'process_low'
+    label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda (params.enable_conda ? "bioconda::multiqc=1.11" : null)
+    conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null)
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
similarity index 100%
rename from modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml
rename to modules/nf-core/custom/dumpsoftwareversions/meta.yml
diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
similarity index 100%
rename from modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
rename to modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
similarity index 85%
rename from modules/nf-core/modules/fastqc/main.nf
rename to modules/nf-core/fastqc/main.nf
index ed6b8c5..0573036 100644
--- a/modules/nf-core/modules/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -44,4 +44,16 @@ process FASTQC {
         END_VERSIONS
         """
     }
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.html
+    touch ${prefix}.zip
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
similarity index 100%
rename from modules/nf-core/modules/fastqc/meta.yml
rename to modules/nf-core/fastqc/meta.yml
diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf
deleted file mode 100644
index 1264aac..0000000
--- a/modules/nf-core/modules/multiqc/main.nf
+++ /dev/null
@@ -1,31 +0,0 @@
-process MULTIQC {
-    label 'process_medium'
-
-    conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null)
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }"
-
-    input:
-    path multiqc_files
-
-    output:
-    path "*multiqc_report.html", emit: report
-    path "*_data"              , emit: data
-    path "*_plots"             , optional:true, emit: plots
-    path "versions.yml"        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    multiqc -f $args .
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
new file mode 100644
index 0000000..a8159a5
--- /dev/null
+++ b/modules/nf-core/multiqc/main.nf
@@ -0,0 +1,53 @@
+process MULTIQC {
+    label 'process_single'
+
+    conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+
+    input:
+    path  multiqc_files, stageAs: "?/*"
+    path(multiqc_config)
+    path(extra_multiqc_config)
+    path(multiqc_logo)
+
+    output:
+    path "*multiqc_report.html", emit: report
+    path "*_data"              , emit: data
+    path "*_plots"             , optional:true, emit: plots
+    path "versions.yml"        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def config = multiqc_config ? "--config $multiqc_config" : ''
+    def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
+    """
+    multiqc \\
+        --force \\
+        $args \\
+        $config \\
+        $extra_config \\
+        .
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch multiqc_data
+    touch multiqc_plots
+    touch multiqc_report.html
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
similarity index 73%
rename from modules/nf-core/modules/multiqc/meta.yml
rename to modules/nf-core/multiqc/meta.yml
index 6fa891e..ebc29b2 100644
--- a/modules/nf-core/modules/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -12,11 +12,25 @@ tools:
       homepage: https://multiqc.info/
       documentation: https://multiqc.info/docs/
       licence: ["GPL-3.0-or-later"]
+
 input:
   - multiqc_files:
       type: file
       description: |
         List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - multiqc_config:
+      type: file
+      description: Optional config yml for MultiQC
+      pattern: "*.{yml,yaml}"
+  - extra_multiqc_config:
+      type: file
+      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
+      pattern: "*.{yml,yaml}"
+  - multiqc_logo:
+      type: file
+      description: Optional logo file for MultiQC
+      pattern: "*.{png}"
+
 output:
   - report:
       type: file
@@ -38,3 +52,4 @@ authors:
   - "@abhi18av"
   - "@bunop"
   - "@drpatelh"
+  - "@jfy133"
diff --git a/nextflow.config b/nextflow.config
index a32ee58..6989b23 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,9 @@ params {
 // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
+    multiqc_logo               = null
     max_multiqc_email_size     = '25.MB'
+    multiqc_methods_description = null
 
     // Boilerplate options
     outdir                     = null
@@ -26,6 +28,7 @@ params {
     email_on_fail              = null
     plaintext_email            = false
     monochrome_logs            = false
+    hook_url                   = null
     help                       = false
     validate_params            = true
     show_hidden_params         = false
@@ -69,7 +72,6 @@ try {
 // }
 
 
-
 profiles {
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
@@ -177,6 +179,7 @@ manifest {
     mainScript      = 'main.nf'
     nextflowVersion = '!>=21.10.3'
     version         = '1.0dev'
+    doi             = ''
 }
 
 // Load modules.config for DSL2 module specific options
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a616d06..5a4d21a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -182,12 +182,30 @@
                     "fa_icon": "fas fa-palette",
                     "hidden": true
                 },
+                "hook_url": {
+                    "type": "string",
+                    "description": "Incoming hook URL for messaging service",
+                    "fa_icon": "fas fa-people-group",
+                    "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.",
+                    "hidden": true
+                },
                 "multiqc_config": {
                     "type": "string",
                     "description": "Custom config file to supply to MultiQC.",
                     "fa_icon": "fas fa-cog",
                     "hidden": true
                 },
+                "multiqc_logo": {
+                    "type": "string",
+                    "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file",
+                    "fa_icon": "fas fa-image",
+                    "hidden": true
+                },
+                "multiqc_methods_description": {
+                    "type": "string",
+                    "description": "Custom MultiQC yaml file containing HTML including a methods description.",
+                    "fa_icon": "fas fa-cog"
+                },
                 "tracedir": {
                     "type": "string",
                     "description": "Directory to keep pipeline Nextflow logs and reports.",
diff --git a/pyproject.toml b/pyproject.toml
new file mode 100644
index 0000000..0d62beb
--- /dev/null
+++ b/pyproject.toml
@@ -0,0 +1,10 @@
+# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black.
+# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
+[tool.black]
+line-length = 120
+target_version = ["py37", "py38", "py39", "py310"]
+
+[tool.isort]
+profile = "black"
+known_first_party = ["nf_core"]
+multi_line_output = 3
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index 748afce..564b9ac 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -23,8 +23,10 @@ if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input sample
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-ch_multiqc_config        = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
+ch_multiqc_config          = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+ch_multiqc_custom_config   = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty()
+ch_multiqc_logo            = params.multiqc_logo   ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty()
+ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -46,9 +48,9 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check'
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { FASTQC                      } from '../modules/nf-core/modules/fastqc/main'
-include { MULTIQC                     } from '../modules/nf-core/modules/multiqc/main'
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
+include { FASTQC                      } from '../modules/nf-core/fastqc/main'
+include { MULTIQC                     } from '../modules/nf-core/multiqc/main'
+include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -89,15 +91,20 @@ workflow INSDCDOWNLOAD {
     workflow_summary    = WorkflowInsdcdownload.paramsSummaryMultiqc(workflow, summary_params)
     ch_workflow_summary = Channel.value(workflow_summary)
 
+    methods_description    = WorkflowInsdcdownload.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description)
+    ch_methods_description = Channel.value(methods_description)
+
     ch_multiqc_files = Channel.empty()
-    ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
     ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'))
     ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
 
     MULTIQC (
-        ch_multiqc_files.collect()
+        ch_multiqc_files.collect(),
+        ch_multiqc_config.collect().ifEmpty([]),
+        ch_multiqc_custom_config.collect().ifEmpty([]),
+        ch_multiqc_logo.collect().ifEmpty([])
     )
     multiqc_report = MULTIQC.out.report.toList()
     ch_versions    = ch_versions.mix(MULTIQC.out.versions)
@@ -114,6 +121,9 @@ workflow.onComplete {
         NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report)
     }
     NfcoreTemplate.summary(workflow, params, log)
+    if (params.hook_url) {
+        NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log)
+    }
 }
 
 /*

From 9e07b9bc28677ff039c2a1e83acc3138052d3a2f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 10:08:08 +0100
Subject: [PATCH 36/85] Template update for nf-core/tools version 2.7

---
 .devcontainer/devcontainer.json               | 27 +++++
 .gitattributes                                |  1 +
 .github/CONTRIBUTING.md                       | 16 +++
 .github/ISSUE_TEMPLATE/bug_report.yml         | 98 +++++++++---------
 .github/workflows/ci.yml                      |  8 +-
 .github/workflows/fix-linting.yml             |  6 +-
 .github/workflows/linting.yml                 | 18 ++--
 .github/workflows/linting_comment.yml         |  2 +-
 .nf-core.yml                                  | 20 ++--
 .prettierignore                               |  2 +
 CITATION.cff                                  | 56 -----------
 README.md                                     |  4 +-
 assets/slackreport.json                       | 34 +++++++
 docs/usage.md                                 | 24 +++--
 lib/NfcoreSchema.groovy                       |  1 -
 lib/NfcoreTemplate.groovy                     | 41 ++++++--
 lib/WorkflowMain.groovy                       | 20 ++--
 modules.json                                  |  9 +-
 modules/local/samplesheet_check.nf            |  6 +-
 .../custom/dumpsoftwareversions/main.nf       |  2 +-
 .../templates/dumpsoftwareversions.py         | 99 ++++++++++---------
 modules/nf-core/fastqc/main.nf                | 40 +++-----
 modules/nf-core/multiqc/main.nf               |  2 +-
 nextflow.config                               | 15 +--
 nextflow_schema.json                          | 30 ++++--
 workflows/insdcdownload.nf                    |  9 +-
 26 files changed, 338 insertions(+), 252 deletions(-)
 create mode 100644 .devcontainer/devcontainer.json
 delete mode 100644 CITATION.cff
 create mode 100644 assets/slackreport.json
 mode change 100644 => 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
new file mode 100644
index 0000000..ea27a58
--- /dev/null
+++ b/.devcontainer/devcontainer.json
@@ -0,0 +1,27 @@
+{
+    "name": "nfcore",
+    "image": "nfcore/gitpod:latest",
+    "remoteUser": "gitpod",
+
+    // Configure tool-specific properties.
+    "customizations": {
+        // Configure properties specific to VS Code.
+        "vscode": {
+            // Set *default* container specific settings.json values on container create.
+            "settings": {
+                "python.defaultInterpreterPath": "/opt/conda/bin/python",
+                "python.linting.enabled": true,
+                "python.linting.pylintEnabled": true,
+                "python.formatting.autopep8Path": "/opt/conda/bin/autopep8",
+                "python.formatting.yapfPath": "/opt/conda/bin/yapf",
+                "python.linting.flake8Path": "/opt/conda/bin/flake8",
+                "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle",
+                "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle",
+                "python.linting.pylintPath": "/opt/conda/bin/pylint"
+            },
+
+            // Add the IDs of extensions you want installed when the container is created.
+            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"]
+        }
+    }
+}
diff --git a/.gitattributes b/.gitattributes
index 050bb12..7a2dabc 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1,3 +1,4 @@
 *.config linguist-language=nextflow
+*.nf.test linguist-language=nextflow
 modules/nf-core/** linguist-generated
 subworkflows/nf-core/** linguist-generated
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index a6fef83..f3f9b2b 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -95,3 +95,19 @@ If you are using a new feature from core Nextflow, you may bump the minimum requ
 ### Images and figures
 
 For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
+
+## GitHub Codespaces
+
+This repo includes a devcontainer configuration which will create a GitHub Codespaces for Nextflow development! This is an online developer environment that runs in your browser, complete with VSCode and a terminal.
+
+To get started:
+
+- Open the repo in [Codespaces](https://github.com/sanger-tol/insdcdownload/codespaces)
+- Tools installed
+  - nf-core
+  - Nextflow
+
+Devcontainer specs:
+
+- [DevContainer config](.devcontainer/devcontainer.json)
+- [Dockerfile](.devcontainer/Dockerfile)
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index e142079..73b6550 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -2,52 +2,52 @@ name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
 body:
-  - type: textarea
-    id: description
-    attributes:
-      label: Description of the bug
-      description: A clear and concise description of what the bug is.
-    validations:
-      required: true
-  - type: textarea
-    id: command_used
-    attributes:
-      label: Command used and terminal output
-      description: Steps to reproduce the behaviour. Please paste the command you used
-        to launch the pipeline and the output from your terminal.
-      render: console
-      placeholder: "$ nextflow run ...
-
-
-        Some output where something broke
-
-        "
-  - type: textarea
-    id: files
-    attributes:
-      label: Relevant files
-      description: "Please drag and drop the relevant files here. Create a `.zip` archive
-        if the extension is not allowed.
-
-        Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
-        in the directory where you launched the pipeline)_ as well as custom Nextflow
-        configuration files.
-
-        "
-  - type: textarea
-    id: system
-    attributes:
-      label: System information
-      description: "* Nextflow version _(eg. 21.10.3)_
-
-        * Hardware _(eg. HPC, Desktop, Cloud)_
-
-        * Executor _(eg. slurm, local, awsbatch)_
-
-        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
-
-        * OS _(eg. CentOS Linux, macOS, Linux Mint)_
-
-        * Version of sanger-tol/insdcdownload _(eg. 1.1, 1.5, 1.8.2)_
-
-        "
+- type: textarea
+  id: description
+  attributes:
+    label: Description of the bug
+    description: A clear and concise description of what the bug is.
+  validations:
+    required: true
+- type: textarea
+  id: command_used
+  attributes:
+    label: Command used and terminal output
+    description: Steps to reproduce the behaviour. Please paste the command you used
+      to launch the pipeline and the output from your terminal.
+    render: console
+    placeholder: '$ nextflow run ...
+
+
+      Some output where something broke
+
+      '
+- type: textarea
+  id: files
+  attributes:
+    label: Relevant files
+    description: 'Please drag and drop the relevant files here. Create a `.zip` archive
+      if the extension is not allowed.
+
+      Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
+      in the directory where you launched the pipeline)_ as well as custom Nextflow
+      configuration files.
+
+      '
+- type: textarea
+  id: system
+  attributes:
+    label: System information
+    description: '* Nextflow version _(eg. 22.10.1)_
+
+      * Hardware _(eg. HPC, Desktop, Cloud)_
+
+      * Executor _(eg. slurm, local, awsbatch)_
+
+      * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
+
+      * OS _(eg. CentOS Linux, macOS, Linux Mint)_
+
+      * Version of sanger-tol/insdcdownload _(eg. 1.1, 1.5, 1.8.2)_
+
+      '
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 19841ae..c2181c3 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -11,6 +11,10 @@ on:
 env:
   NXF_ANSI_LOG: false
 
+concurrency:
+  group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}"
+  cancel-in-progress: true
+
 jobs:
   test:
     name: Run pipeline with test data
@@ -20,11 +24,11 @@ jobs:
     strategy:
       matrix:
         NXF_VER:
-          - "21.10.3"
+          - "22.10.1"
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v2
+        uses: actions/checkout@v3
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 1c18c1d..d8478c7 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -24,7 +24,7 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      - uses: actions/setup-node@v2
+      - uses: actions/setup-node@v3
 
       - name: Install Prettier
         run: npm install -g prettier @prettier/plugin-php
@@ -34,9 +34,9 @@ jobs:
         id: prettier_status
         run: |
           if prettier --check ${GITHUB_WORKSPACE}; then
-            echo "::set-output name=result::pass"
+            echo "result=pass" >> $GITHUB_OUTPUT
           else
-            echo "::set-output name=result::fail"
+            echo "result=fail" >> $GITHUB_OUTPUT
           fi
 
       - name: Run 'prettier --write'
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 8a5ce69..858d622 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -4,6 +4,8 @@ name: nf-core linting
 # that the code meets the nf-core guidelines.
 on:
   push:
+    branches:
+      - dev
   pull_request:
   release:
     types: [published]
@@ -12,9 +14,9 @@ jobs:
   EditorConfig:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v2
+      - uses: actions/checkout@v3
 
-      - uses: actions/setup-node@v2
+      - uses: actions/setup-node@v3
 
       - name: Install editorconfig-checker
         run: npm install -g editorconfig-checker
@@ -25,9 +27,9 @@ jobs:
   Prettier:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v2
+      - uses: actions/checkout@v3
 
-      - uses: actions/setup-node@v2
+      - uses: actions/setup-node@v3
 
       - name: Install Prettier
         run: npm install -g prettier
@@ -38,7 +40,7 @@ jobs:
   PythonBlack:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v2
+      - uses: actions/checkout@v3
 
       - name: Check code lints with Black
         uses: psf/black@stable
@@ -69,12 +71,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@v2
+        uses: actions/checkout@v3
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v1
 
-      - uses: actions/setup-python@v3
+      - uses: actions/setup-python@v4
         with:
           python-version: "3.7"
           architecture: "x64"
@@ -97,7 +99,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@v2
+        uses: actions/upload-artifact@v3
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 04758f6..0bbcd30 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -18,7 +18,7 @@ jobs:
 
       - name: Get PR number
         id: pr_number
-        run: echo "::set-output name=pr_number::$(cat linting-logs/PR_number.txt)"
+        run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
         uses: marocchino/sticky-pull-request-comment@v2
diff --git a/.nf-core.yml b/.nf-core.yml
index eb63018..ae51a08 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,15 +1,15 @@
 repository_type: pipeline
 lint:
   files_exist:
-    - CODE_OF_CONDUCT.md
-    - assets/nf-core-insdcdownload_logo_light.png
-    - docs/images/nf-core-insdcdownload_logo_light.png
-    - docs/images/nf-core-insdcdownload_logo_dark.png
-    - .github/ISSUE_TEMPLATE/config.yml
-    - .github/workflows/awstest.yml
-    - .github/workflows/awsfulltest.yml
+  - CODE_OF_CONDUCT.md
+  - assets/nf-core-insdcdownload_logo_light.png
+  - docs/images/nf-core-insdcdownload_logo_light.png
+  - docs/images/nf-core-insdcdownload_logo_dark.png
+  - .github/ISSUE_TEMPLATE/config.yml
+  - .github/workflows/awstest.yml
+  - .github/workflows/awsfulltest.yml
   nextflow_config:
-    - manifest.name
-    - manifest.homePage
+  - manifest.name
+  - manifest.homePage
   multiqc_config:
-    - report_comment
+  - report_comment
diff --git a/.prettierignore b/.prettierignore
index eb74a57..437d763 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -1,5 +1,6 @@
 email_template.html
 adaptivecard.json
+slackreport.json
 .nextflow*
 work/
 data/
@@ -8,3 +9,4 @@ results/
 testing/
 testing*
 *.pyc
+bin/
diff --git a/CITATION.cff b/CITATION.cff
deleted file mode 100644
index 017666c..0000000
--- a/CITATION.cff
+++ /dev/null
@@ -1,56 +0,0 @@
-cff-version: 1.2.0
-message: "If you use `nf-core tools` in your work, please cite the `nf-core` publication"
-authors:
-  - family-names: Ewels
-    given-names: Philip
-  - family-names: Peltzer
-    given-names: Alexander
-  - family-names: Fillinger
-    given-names: Sven
-  - family-names: Patel
-    given-names: Harshil
-  - family-names: Alneberg
-    given-names: Johannes
-  - family-names: Wilm
-    given-names: Andreas
-  - family-names: Garcia
-    given-names: Maxime Ulysse
-  - family-names: Di Tommaso
-    given-names: Paolo
-  - family-names: Nahnsen
-    given-names: Sven
-title: "The nf-core framework for community-curated bioinformatics pipelines."
-version: 2.4.1
-doi: 10.1038/s41587-020-0439-x
-date-released: 2022-05-16
-url: https://github.com/nf-core/tools
-prefered-citation:
-  type: article
-  authors:
-    - family-names: Ewels
-      given-names: Philip
-    - family-names: Peltzer
-      given-names: Alexander
-    - family-names: Fillinger
-      given-names: Sven
-    - family-names: Patel
-      given-names: Harshil
-    - family-names: Alneberg
-      given-names: Johannes
-    - family-names: Wilm
-      given-names: Andreas
-    - family-names: Garcia
-      given-names: Maxime Ulysse
-    - family-names: Di Tommaso
-      given-names: Paolo
-    - family-names: Nahnsen
-      given-names: Sven
-  doi: 10.1038/s41587-020-0439-x
-  journal: nature biotechnology
-  start: 276
-  end: 278
-  title: "The nf-core framework for community-curated bioinformatics pipelines."
-  issue: 3
-  volume: 38
-  year: 2020
-  url: https://dx.doi.org/10.1038/s41587-020-0439-x
diff --git a/README.md b/README.md
index 68ee811..b5fee69 100644
--- a/README.md
+++ b/README.md
@@ -1,6 +1,6 @@
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -27,7 +27,7 @@ On release, automated continuous integration tests run the pipeline on a full-si
 
 ## Quick Start
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`)
+1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`)
 
 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
 
diff --git a/assets/slackreport.json b/assets/slackreport.json
new file mode 100644
index 0000000..043d02f
--- /dev/null
+++ b/assets/slackreport.json
@@ -0,0 +1,34 @@
+{
+    "attachments": [
+        {
+            "fallback": "Plain-text summary of the attachment.",
+            "color": "<% if (success) { %>good<% } else { %>danger<%} %>",
+            "author_name": "sanger-tol/readmapping v${version} - ${runName}",
+            "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico",
+            "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>",
+            "fields": [
+                {
+                    "title": "Command used to launch the workflow",
+                    "value": "```${commandLine}```",
+                    "short": false
+                }
+                <%
+                    if (!success) { %>
+                    ,
+                    {
+                        "title": "Full error message",
+                        "value": "```${errorReport}```",
+                        "short": false
+                    },
+                    {
+                        "title": "Pipeline configuration",
+                        "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>",
+                        "short": false
+                    }
+                    <% }
+                %>
+            ],
+            "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})"
+        }
+    ]
+}
diff --git a/docs/usage.md b/docs/usage.md
index 58365c3..34f317e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -81,9 +81,9 @@ nextflow pull sanger-tol/insdcdownload
 
 It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
 
-First, go to the [sanger-tol/insdcdownload releases page](https://github.com/sanger-tol/insdcdownload/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`.
+First, go to the [sanger-tol/insdcdownload releases page](https://github.com/sanger-tol/insdcdownload/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag.
 
-This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future.
+This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
 
 ## Core Nextflow arguments
 
@@ -93,7 +93,7 @@ This version number will be logged in reports when you run the pipeline, so that
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/).
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -102,8 +102,11 @@ The pipeline also dynamically loads configurations from [https://github.com/nf-c
 Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
 They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
-If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended.
+If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment.
 
+- `test`
+  - A profile with a complete configuration for automated testing
+  - Includes links to test data so needs no other parameters
 - `docker`
   - A generic configuration profile to be used with [Docker](https://docker.com/)
 - `singularity`
@@ -116,9 +119,6 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
 - `conda`
   - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
-- `test`
-  - A profile with a complete configuration for automated testing
-  - Includes links to test data so needs no other parameters
 
 ### `-resume`
 
@@ -167,8 +167,14 @@ Work dir:
 Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
 ```
 
+#### For beginners
+
+A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below.
+
+#### Advanced option on process level
+
 To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
-We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`.
+We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`.
 If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
 The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
 The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
@@ -187,7 +193,7 @@ process {
 >
 > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
 
-### Updating containers
+### Updating containers (advanced users)
 
 The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
 
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index b3d092f..33cd4f6 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -46,7 +46,6 @@ class NfcoreSchema {
             'quiet',
             'syslog',
             'v',
-            'version',
 
             // Options for `nextflow run` command
             'ansi',
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index 45992fa..2023f45 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -32,6 +32,25 @@ class NfcoreTemplate {
         }
     }
 
+    //
+    // Generate version string
+    //
+    public static String version(workflow) {
+        String version_string = ""
+
+        if (workflow.manifest.version) {
+            def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
+            version_string += "${prefix_v}${workflow.manifest.version}"
+        }
+
+        if (workflow.commitId) {
+            def git_shortsha = workflow.commitId.substring(0, 7)
+            version_string += "-g${git_shortsha}"
+        }
+
+        return version_string
+    }
+
     //
     // Construct and send completion email
     //
@@ -61,7 +80,7 @@ class NfcoreTemplate {
         misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
 
         def email_fields = [:]
-        email_fields['version']      = workflow.manifest.version
+        email_fields['version']      = NfcoreTemplate.version(workflow)
         email_fields['runName']      = workflow.runName
         email_fields['success']      = workflow.success
         email_fields['dateComplete'] = workflow.complete
@@ -146,10 +165,10 @@ class NfcoreTemplate {
     }
 
     //
-    // Construct and send adaptive card
-    // https://adaptivecards.io
+    // Construct and send a notification to a web server as JSON
+    // e.g. Microsoft Teams and Slack
     //
-    public static void adaptivecard(workflow, params, summary_params, projectDir, log) {
+    public static void IM_notification(workflow, params, summary_params, projectDir, log) {
         def hook_url = params.hook_url
 
         def summary = [:]
@@ -170,7 +189,7 @@ class NfcoreTemplate {
         misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
 
         def msg_fields = [:]
-        msg_fields['version']      = workflow.manifest.version
+        msg_fields['version']      = NfcoreTemplate.version(workflow)
         msg_fields['runName']      = workflow.runName
         msg_fields['success']      = workflow.success
         msg_fields['dateComplete'] = workflow.complete
@@ -178,13 +197,16 @@ class NfcoreTemplate {
         msg_fields['exitStatus']   = workflow.exitStatus
         msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None')
         msg_fields['errorReport']  = (workflow.errorReport ?: 'None')
-        msg_fields['commandLine']  = workflow.commandLine
+        msg_fields['commandLine']  = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "")
         msg_fields['projectDir']   = workflow.projectDir
         msg_fields['summary']      = summary << misc_fields
 
         // Render the JSON template
         def engine       = new groovy.text.GStringTemplateEngine()
-        def hf = new File("$projectDir/assets/adaptivecard.json")
+        // Different JSON depending on the service provider
+        // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format
+        def json_path     = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json"
+        def hf            = new File("$projectDir/assets/${json_path}")
         def json_template = engine.createTemplate(hf).make(msg_fields)
         def json_message  = json_template.toString()
 
@@ -209,7 +231,7 @@ class NfcoreTemplate {
             if (workflow.stats.ignoredCount == 0) {
                 log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
             } else {
-                log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+                log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
             }
         } else {
             log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
@@ -297,10 +319,11 @@ class NfcoreTemplate {
     //
     public static String logo(workflow, monochrome_logs) {
         Map colors = logColours(monochrome_logs)
+        String workflow_version = NfcoreTemplate.version(workflow)
         String.format(
             """\n
             ${dashedLine(monochrome_logs)}
-            ${colors.purple}  ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset}
+            ${colors.purple}  ${workflow.manifest.name} ${workflow_version}${colors.reset}
             ${dashedLine(monochrome_logs)}
             """.stripIndent()
         )
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 21c861a..9b132d5 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -19,7 +19,7 @@ class WorkflowMain {
     }
 
     //
-    // Print help to screen if required
+    // Generate help string
     //
     public static String help(workflow, params, log) {
         def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --fasta reference.fa -profile docker"
@@ -32,7 +32,7 @@ class WorkflowMain {
     }
 
     //
-    // Print parameter summary log to screen
+    // Generate parameter summary log string
     //
     public static String paramsSummaryLog(workflow, params, log) {
         def summary_log = ''
@@ -53,20 +53,26 @@ class WorkflowMain {
             System.exit(0)
         }
 
-        // Validate workflow parameters via the JSON schema
-        if (params.validate_params) {
-            NfcoreSchema.validateParameters(workflow, params, log)
+        // Print workflow version and exit on --version
+        if (params.version) {
+            String workflow_version = NfcoreTemplate.version(workflow)
+            log.info "${workflow.manifest.name} ${workflow_version}"
+            System.exit(0)
         }
 
         // Print parameter summary log to screen
-
         log.info paramsSummaryLog(workflow, params, log)
 
+        // Validate workflow parameters via the JSON schema
+        if (params.validate_params) {
+            NfcoreSchema.validateParameters(workflow, params, log)
+        }
+
         // Check that a -profile or Nextflow config has been provided to run the pipeline
         NfcoreTemplate.checkConfigProvided(workflow, log)
 
         // Check that conda channels are set-up correctly
-        if (params.enable_conda) {
+        if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
             Utils.checkCondaChannels(log)
         }
 
diff --git a/modules.json b/modules.json
index 3ae60e0..ae42760 100644
--- a/modules.json
+++ b/modules.json
@@ -7,15 +7,18 @@
                 "nf-core": {
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
+                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "installed_by": ["modules"]
                     }
                 }
             }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index 910e7ae..a4a4d1a 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -1,7 +1,8 @@
 process SAMPLESHEET_CHECK {
     tag "$samplesheet"
+    label 'process_single'
 
-    conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
+    conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
         'quay.io/biocontainers/python:3.8.3' }"
@@ -13,6 +14,9 @@ process SAMPLESHEET_CHECK {
     path '*.csv'       , emit: csv
     path "versions.yml", emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script: // This script is bundled with the pipeline, in sanger-tol/insdcdownload/bin/
     """
     check_samplesheet.py \\
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index cebb6e0..3df2176 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,7 +2,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null)
+    conda "bioconda::multiqc=1.13"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
         'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
old mode 100644
new mode 100755
index 787bdb7..e55b8d4
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -1,5 +1,9 @@
 #!/usr/bin/env python
 
+
+"""Provide functions to merge multiple versions.yml files."""
+
+
 import platform
 from textwrap import dedent
 
@@ -7,6 +11,7 @@
 
 
 def _make_versions_html(versions):
+    """Generate a tabular HTML output of all versions for MultiQC."""
     html = [
         dedent(
             """\\
@@ -45,47 +50,53 @@ def _make_versions_html(versions):
     return "\\n".join(html)
 
 
-versions_this_module = {}
-versions_this_module["${task.process}"] = {
-    "python": platform.python_version(),
-    "yaml": yaml.__version__,
-}
-
-with open("$versions") as f:
-    versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
-
-# aggregate versions by the module name (derived from fully-qualified process name)
-versions_by_module = {}
-for process, process_versions in versions_by_process.items():
-    module = process.split(":")[-1]
-    try:
-        if versions_by_module[module] != process_versions:
-            raise AssertionError(
-                "We assume that software versions are the same between all modules. "
-                "If you see this error-message it means you discovered an edge-case "
-                "and should open an issue in nf-core/tools. "
-            )
-    except KeyError:
-        versions_by_module[module] = process_versions
-
-versions_by_module["Workflow"] = {
-    "Nextflow": "$workflow.nextflow.version",
-    "$workflow.manifest.name": "$workflow.manifest.version",
-}
-
-versions_mqc = {
-    "id": "software_versions",
-    "section_name": "${workflow.manifest.name} Software Versions",
-    "section_href": "https://github.com/${workflow.manifest.name}",
-    "plot_type": "html",
-    "description": "are collected at run time from the software output.",
-    "data": _make_versions_html(versions_by_module),
-}
-
-with open("software_versions.yml", "w") as f:
-    yaml.dump(versions_by_module, f, default_flow_style=False)
-with open("software_versions_mqc.yml", "w") as f:
-    yaml.dump(versions_mqc, f, default_flow_style=False)
-
-with open("versions.yml", "w") as f:
-    yaml.dump(versions_this_module, f, default_flow_style=False)
+def main():
+    """Load all version files and generate merged output."""
+    versions_this_module = {}
+    versions_this_module["${task.process}"] = {
+        "python": platform.python_version(),
+        "yaml": yaml.__version__,
+    }
+
+    with open("$versions") as f:
+        versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module
+
+    # aggregate versions by the module name (derived from fully-qualified process name)
+    versions_by_module = {}
+    for process, process_versions in versions_by_process.items():
+        module = process.split(":")[-1]
+        try:
+            if versions_by_module[module] != process_versions:
+                raise AssertionError(
+                    "We assume that software versions are the same between all modules. "
+                    "If you see this error-message it means you discovered an edge-case "
+                    "and should open an issue in nf-core/tools. "
+                )
+        except KeyError:
+            versions_by_module[module] = process_versions
+
+    versions_by_module["Workflow"] = {
+        "Nextflow": "$workflow.nextflow.version",
+        "$workflow.manifest.name": "$workflow.manifest.version",
+    }
+
+    versions_mqc = {
+        "id": "software_versions",
+        "section_name": "${workflow.manifest.name} Software Versions",
+        "section_href": "https://github.com/${workflow.manifest.name}",
+        "plot_type": "html",
+        "description": "are collected at run time from the software output.",
+        "data": _make_versions_html(versions_by_module),
+    }
+
+    with open("software_versions.yml", "w") as f:
+        yaml.dump(versions_by_module, f, default_flow_style=False)
+    with open("software_versions_mqc.yml", "w") as f:
+        yaml.dump(versions_mqc, f, default_flow_style=False)
+
+    with open("versions.yml", "w") as f:
+        yaml.dump(versions_this_module, f, default_flow_style=False)
+
+
+if __name__ == "__main__":
+    main()
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 0573036..9ae5838 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -2,7 +2,7 @@ process FASTQC {
     tag "$meta.id"
     label 'process_medium'
 
-    conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
+    conda "bioconda::fastqc=0.11.9"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
         'quay.io/biocontainers/fastqc:0.11.9--0' }"
@@ -20,30 +20,22 @@ process FASTQC {
 
     script:
     def args = task.ext.args ?: ''
-    // Add soft-links to original FastQs for consistent naming in pipeline
     def prefix = task.ext.prefix ?: "${meta.id}"
-    if (meta.single_end) {
-        """
-        [ ! -f  ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
-        fastqc $args --threads $task.cpus ${prefix}.fastq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
-        END_VERSIONS
-        """
-    } else {
-        """
-        [ ! -f  ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
-        [ ! -f  ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
-        fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
-        END_VERSIONS
-        """
-    }
+    // Make list of old name and new name pairs to use for renaming in the bash while loop
+    def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
+    def rename_to = old_new_pairs*.join(' ').join(' ')
+    def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+    """
+    printf "%s %s\\n" $rename_to | while read old_name new_name; do
+        [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
+    done
+    fastqc $args --threads $task.cpus $renamed_files
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
+    END_VERSIONS
+    """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index a8159a5..68f66be 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,7 +1,7 @@
 process MULTIQC {
     label 'process_single'
 
-    conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null)
+    conda "bioconda::multiqc=1.13"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
         'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
diff --git a/nextflow.config b/nextflow.config
index 6989b23..6d815bd 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,10 +30,10 @@ params {
     monochrome_logs            = false
     hook_url                   = null
     help                       = false
+    version                    = false
     validate_params            = true
     show_hidden_params         = false
     schema_ignore_params       = 'genomes'
-    enable_conda               = false
 
 
     // Config options
@@ -75,7 +75,7 @@ try {
 profiles {
     debug { process.beforeScript = 'echo $HOSTNAME' }
     conda {
-        params.enable_conda    = true
+        conda.enabled          = true
         docker.enabled         = false
         singularity.enabled    = false
         podman.enabled         = false
@@ -83,7 +83,7 @@ profiles {
         charliecloud.enabled   = false
     }
     mamba {
-        params.enable_conda    = true
+        conda.enabled          = true
         conda.useMamba         = true
         docker.enabled         = false
         singularity.enabled    = false
@@ -99,6 +99,9 @@ profiles {
         shifter.enabled        = false
         charliecloud.enabled   = false
     }
+    arm {
+        docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
+    }
     singularity {
         singularity.enabled    = true
         singularity.autoMounts = true
@@ -173,11 +176,11 @@ dag {
 
 manifest {
     name            = 'sanger-tol/insdcdownload'
-    author          = '@muffato'
+    author          = """@muffato"""
     homePage        = 'https://github.com/sanger-tol/insdcdownload'
-    description     = 'Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure'
+    description     = """Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure"""
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=21.10.3'
+    nextflowVersion = '!>=22.10.1'
     version         = '1.0dev'
     doi             = ''
 }
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 5a4d21a..ec23f27 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -10,7 +10,10 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["input", "outdir"],
+            "required": [
+                "input",
+                "outdir"
+            ],
             "properties": {
                 "input": {
                     "type": "string",
@@ -145,13 +148,26 @@
                     "fa_icon": "fas fa-question-circle",
                     "hidden": true
                 },
+                "version": {
+                    "type": "boolean",
+                    "description": "Display version and exit.",
+                    "fa_icon": "fas fa-question-circle",
+                    "hidden": true
+                },
                 "publish_dir_mode": {
                     "type": "string",
                     "default": "copy",
                     "description": "Method used to save pipeline results to output directory.",
                     "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
                     "fa_icon": "fas fa-copy",
-                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
+                    "enum": [
+                        "symlink",
+                        "rellink",
+                        "link",
+                        "copy",
+                        "copyNoFollow",
+                        "move"
+                    ],
                     "hidden": true
                 },
                 "email_on_fail": {
@@ -186,7 +202,7 @@
                     "type": "string",
                     "description": "Incoming hook URL for messaging service",
                     "fa_icon": "fas fa-people-group",
-                    "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.",
+                    "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.",
                     "hidden": true
                 },
                 "multiqc_config": {
@@ -226,12 +242,6 @@
                     "description": "Show all params when using `--help`",
                     "hidden": true,
                     "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
-                },
-                "enable_conda": {
-                    "type": "boolean",
-                    "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.",
-                    "hidden": true,
-                    "fa_icon": "fas fa-bacon"
                 }
             }
         }
@@ -253,4 +263,4 @@
             "$ref": "#/definitions/generic_options"
         }
     ]
-}
+}
\ No newline at end of file
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index 564b9ac..951e492 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -102,12 +102,11 @@ workflow INSDCDOWNLOAD {
 
     MULTIQC (
         ch_multiqc_files.collect(),
-        ch_multiqc_config.collect().ifEmpty([]),
-        ch_multiqc_custom_config.collect().ifEmpty([]),
-        ch_multiqc_logo.collect().ifEmpty([])
+        ch_multiqc_config.toList(),
+        ch_multiqc_custom_config.toList(),
+        ch_multiqc_logo.toList()
     )
     multiqc_report = MULTIQC.out.report.toList()
-    ch_versions    = ch_versions.mix(MULTIQC.out.versions)
 }
 
 /*
@@ -122,7 +121,7 @@ workflow.onComplete {
     }
     NfcoreTemplate.summary(workflow, params, log)
     if (params.hook_url) {
-        NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log)
+        NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log)
     }
 }
 

From 93863d6fab715b1d740b7bc7acd8d550e242eac0 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 10:23:27 +0100
Subject: [PATCH 37/85] Template update for nf-core/tools version 2.8

---
 .editorconfig                                 |   2 +-
 .github/ISSUE_TEMPLATE/bug_report.yml         |   3 +-
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/clean-up.yml                |  24 ++++
 .github/workflows/linting.yml                 |   2 +-
 .pre-commit-config.yaml                       |   5 +
 README.md                                     |  64 +++++----
 bin/check_samplesheet.py                      |   3 -
 conf/base.config                              |   2 +-
 conf/test_full.config                         |   2 +
 docs/usage.md                                 | 130 +++++-------------
 lib/NfcoreSchema.groovy                       |   4 +-
 lib/WorkflowInsdcdownload.groovy              |   4 +-
 lib/WorkflowMain.groovy                       |  13 +-
 modules.json                                  |   4 +-
 modules/local/samplesheet_check.nf            |   2 +-
 .../custom/dumpsoftwareversions/main.nf       |   6 +-
 .../custom/dumpsoftwareversions/meta.yml      |   2 +
 modules/nf-core/multiqc/main.nf               |   6 +-
 modules/nf-core/multiqc/meta.yml              |   3 +-
 nextflow.config                               |  29 +++-
 pipeline_template.yml                         |   3 +
 tower.yml                                     |   5 +
 23 files changed, 173 insertions(+), 147 deletions(-)
 create mode 100644 .github/workflows/clean-up.yml
 create mode 100644 .pre-commit-config.yaml
 create mode 100644 pipeline_template.yml
 create mode 100644 tower.yml

diff --git a/.editorconfig b/.editorconfig
index b78de6e..b6b3190 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -8,7 +8,7 @@ trim_trailing_whitespace = true
 indent_size = 4
 indent_style = space
 
-[*.{md,yml,yaml,html,css,scss,js,cff}]
+[*.{md,yml,yaml,html,css,scss,js}]
 indent_size = 2
 
 # These files are edited and tested upstream in nf-core/modules
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 73b6550..06441d4 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -44,7 +44,8 @@ body:
 
       * Executor _(eg. slurm, local, awsbatch)_
 
-      * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_
+      * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud,
+      or Apptainer)_
 
       * OS _(eg. CentOS Linux, macOS, Linux Mint)_
 
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index c08e134..9113a81 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -13,7 +13,7 @@ jobs:
       - name: Check PRs
         if: github.repository == 'sanger-tol/insdcdownload'
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name }} == sanger-tol/insdcdownload ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+          { [[ ${{github.event.pull_request.head.repo.full_name }} == sanger-tol/insdcdownload ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
       # If the above check failed, post a comment on the PR explaining the failure
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
new file mode 100644
index 0000000..694e90e
--- /dev/null
+++ b/.github/workflows/clean-up.yml
@@ -0,0 +1,24 @@
+name: "Close user-tagged issues and PRs"
+on:
+  schedule:
+    - cron: "0 0 * * 0" # Once a week
+
+jobs:
+  clean-up:
+    runs-on: ubuntu-latest
+    permissions:
+      issues: write
+      pull-requests: write
+    steps:
+      - uses: actions/stale@v7
+        with:
+          stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
+          stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
+          close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity."
+          days-before-stale: 30
+          days-before-close: 20
+          days-before-pr-close: -1
+          any-of-labels: "awaiting-changes,awaiting-feedback"
+          exempt-issue-labels: "WIP"
+          exempt-pr-labels: "WIP"
+          repo-token: "${{ secrets.GITHUB_TOKEN }}"
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 858d622..888cb4b 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -78,7 +78,7 @@ jobs:
 
       - uses: actions/setup-python@v4
         with:
-          python-version: "3.7"
+          python-version: "3.8"
           architecture: "x64"
 
       - name: Install dependencies
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
new file mode 100644
index 0000000..0c31cdb
--- /dev/null
+++ b/.pre-commit-config.yaml
@@ -0,0 +1,5 @@
+repos:
+  - repo: https://github.com/pre-commit/mirrors-prettier
+    rev: "v2.7.1"
+    hooks:
+      - id: prettier
diff --git a/README.md b/README.md
index b5fee69..b23f7db 100644
--- a/README.md
+++ b/README.md
@@ -8,49 +8,59 @@
 
 ## Introduction
 
-<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
+**sanger-tol/insdcdownload** is a bioinformatics pipeline that ...
 
-**sanger-tol/insdcdownload** is a pipeline that downloads assemblies from INSDC into a Tree of Life directory structure.
-
-The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
-
-<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
-
-On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.
-
-## Pipeline summary
+<!-- TODO nf-core:
+   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the
+   major pipeline sections and the types of output it produces. You're giving an overview to someone new
+   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction
+-->
 
+<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
+     workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->
 <!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
 
 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
 2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
-## Quick Start
+## Usage
+
+> **Note**
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how
+> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
+> with `-profile test` before running the workflow on actual data.
+
+<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
+     Explain what rows and columns represent. For instance (please edit as appropriate):
 
-1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`)
+First, prepare a samplesheet with your input data that looks as follows:
 
-2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_.
+`samplesheet.csv`:
 
-3. Download the pipeline and test it on a minimal dataset with a single command:
+```csv
+sample,fastq_1,fastq_2
+CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
+```
 
-   ```bash
-   nextflow run sanger-tol/insdcdownload -profile test,YOURPROFILE --outdir <OUTDIR>
-   ```
+Each row represents a fastq file (single-end) or a pair of fastq files (paired end).
 
-   Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
+-->
 
-   > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
-   > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
-   > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
-   > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
+Now, you can run the pipeline using:
 
-4. Start running your own analysis!
+<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->
 
-   <!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
+```bash
+nextflow run sanger-tol/insdcdownload \
+   -profile <docker/singularity/.../institute> \
+   --input samplesheet.csv \
+   --outdir <OUTDIR>
+```
 
-   ```bash
-   nextflow run sanger-tol/insdcdownload --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
-   ```
+> **Warning:**
+> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those
+> provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
+> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
 
 ## Credits
 
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 11b1557..4a758fe 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -158,9 +158,6 @@ def sniff_format(handle):
     peek = read_head(handle)
     handle.seek(0)
     sniffer = csv.Sniffer()
-    if not sniffer.has_header(peek):
-        logger.critical("The given sample sheet does not appear to contain a header.")
-        sys.exit(1)
     dialect = sniffer.sniff(peek)
     return dialect
 
diff --git a/conf/base.config b/conf/base.config
index e6ea465..3a67fb1 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -15,7 +15,7 @@ process {
     memory = { check_max( 6.GB * task.attempt, 'memory' ) }
     time   = { check_max( 4.h  * task.attempt, 'time'   ) }
 
-    errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' }
+    errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
diff --git a/conf/test_full.config b/conf/test_full.config
index c4d71f7..952ecbe 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -10,6 +10,8 @@
 ----------------------------------------------------------------------------------------
 */
 
+cleanup = true
+
 params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'
diff --git a/docs/usage.md b/docs/usage.md
index 34f317e..b98bfaf 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -69,6 +69,29 @@ work                # Directory containing the nextflow working files
 # Other nextflow hidden files, eg. history of pipeline runs and old logs.
 ```
 
+If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file.
+
+Pipeline settings can be provided in a `yaml` or `json` file via `-params-file <file>`.
+
+> ⚠️ Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
+> The above pipeline run specified with a params file in yaml format:
+
+```bash
+nextflow run sanger-tol/insdcdownload -profile docker -params-file params.yaml
+```
+
+with `params.yaml` containing:
+
+```yaml
+input: './samplesheet.csv'
+outdir: './results/'
+genome: 'GRCh37'
+input: 'data'
+<...>
+```
+
+You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).
+
 ### Updating the pipeline
 
 When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
@@ -85,6 +108,10 @@ First, go to the [sanger-tol/insdcdownload releases page](https://github.com/san
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
 
+To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
+
+> 💡 If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
+
 ## Core Nextflow arguments
 
 > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
@@ -93,7 +120,7 @@ This version number will be logged in reports when you run the pipeline, so that
 
 Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
 
-Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below.
+Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
 
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
@@ -117,8 +144,10 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
 - `charliecloud`
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
+- `apptainer`
+  - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `conda`
-  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud.
+  - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 
 ### `-resume`
 
@@ -136,102 +165,19 @@ Specify the path to a specific config file (this is a core Nextflow command). Se
 
 Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
 
-For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
-
-```console
-[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
-Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
-
-Caused by:
-    Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
-
-Command executed:
-    STAR \
-        --genomeDir star \
-        --readFilesIn WT_REP1_trimmed.fq.gz  \
-        --runThreadN 2 \
-        --outFileNamePrefix WT_REP1. \
-        <TRUNCATED>
-
-Command exit status:
-    137
-
-Command output:
-    (empty)
-
-Command error:
-    .command.sh: line 9:  30 Killed    STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. <TRUNCATED>
-Work dir:
-    /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb
-
-Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
-```
-
-#### For beginners
-
-A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below.
-
-#### Advanced option on process level
-
-To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
-We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`.
-If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
-The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
-The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
-Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
-The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
-
-```nextflow
-process {
-    withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
-        memory = 100.GB
-    }
-}
-```
-
-> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
->
-> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
-
-### Updating containers (advanced users)
-
-The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`.
-
-1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19)
-2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags)
-3. Create the custom config accordingly:
-
-   - For Docker:
+To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website.
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0'
-         }
-     }
-     ```
+### Custom Containers
 
-   - For Singularity:
+In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date.
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0'
-         }
-     }
-     ```
+To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
 
-   - For Conda:
+### Custom Tool Arguments
 
-     ```nextflow
-     process {
-         withName: PANGOLIN {
-             conda = 'bioconda::pangolin=3.0.5'
-         }
-     }
-     ```
+A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default.
 
-> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch.
+To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website.
 
 ### nf-core/configs
 
diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy
index 33cd4f6..9b34804 100755
--- a/lib/NfcoreSchema.groovy
+++ b/lib/NfcoreSchema.groovy
@@ -2,6 +2,7 @@
 // This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template.
 //
 
+import nextflow.Nextflow
 import org.everit.json.schema.Schema
 import org.everit.json.schema.loader.SchemaLoader
 import org.everit.json.schema.ValidationException
@@ -83,6 +84,7 @@ class NfcoreSchema {
             'stub-run',
             'test',
             'w',
+            'with-apptainer',
             'with-charliecloud',
             'with-conda',
             'with-dag',
@@ -177,7 +179,7 @@ class NfcoreSchema {
         }
 
         if (has_error) {
-            System.exit(1)
+            Nextflow.error('Exiting!')
         }
     }
 
diff --git a/lib/WorkflowInsdcdownload.groovy b/lib/WorkflowInsdcdownload.groovy
index d5d0f5d..9d211b8 100755
--- a/lib/WorkflowInsdcdownload.groovy
+++ b/lib/WorkflowInsdcdownload.groovy
@@ -2,6 +2,7 @@
 // This file holds several functions specific to the workflow/insdcdownload.nf in the sanger-tol/insdcdownload pipeline
 //
 
+import nextflow.Nextflow
 import groovy.text.SimpleTemplateEngine
 
 class WorkflowInsdcdownload {
@@ -13,8 +14,7 @@ class WorkflowInsdcdownload {
         
 
         if (!params.fasta) {
-            log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
-            System.exit(1)
+            Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file."
         }
     }
 
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 9b132d5..6f9bfff 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -2,6 +2,8 @@
 // This file holds several functions specific to the main.nf workflow in the sanger-tol/insdcdownload pipeline
 //
 
+import nextflow.Nextflow
+
 class WorkflowMain {
 
     //
@@ -21,7 +23,7 @@ class WorkflowMain {
     //
     // Generate help string
     //
-    public static String help(workflow, params, log) {
+    public static String help(workflow, params) {
         def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --fasta reference.fa -profile docker"
         def help_string = ''
         help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs)
@@ -34,7 +36,7 @@ class WorkflowMain {
     //
     // Generate parameter summary log string
     //
-    public static String paramsSummaryLog(workflow, params, log) {
+    public static String paramsSummaryLog(workflow, params) {
         def summary_log = ''
         summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs)
         summary_log += NfcoreSchema.paramsSummaryLog(workflow, params)
@@ -49,7 +51,7 @@ class WorkflowMain {
     public static void initialise(workflow, params, log) {
         // Print help to screen if required
         if (params.help) {
-            log.info help(workflow, params, log)
+            log.info help(workflow, params)
             System.exit(0)
         }
 
@@ -61,7 +63,7 @@ class WorkflowMain {
         }
 
         // Print parameter summary log to screen
-        log.info paramsSummaryLog(workflow, params, log)
+        log.info paramsSummaryLog(workflow, params)
 
         // Validate workflow parameters via the JSON schema
         if (params.validate_params) {
@@ -81,8 +83,7 @@ class WorkflowMain {
 
         // Check input has been provided
         if (!params.input) {
-            log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'"
-            System.exit(1)
+            Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'")
         }
     }
     }
diff --git a/modules.json b/modules.json
index ae42760..dc96cc3 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
@@ -17,7 +17,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c",
+                        "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf
index a4a4d1a..78ef1b6 100644
--- a/modules/local/samplesheet_check.nf
+++ b/modules/local/samplesheet_check.nf
@@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK {
     conda "conda-forge::python=3.8.3"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.8.3' :
-        'quay.io/biocontainers/python:3.8.3' }"
+        'biocontainers/python:3.8.3' }"
 
     input:
     path samplesheet
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 3df2176..800a609 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     label 'process_single'
 
     // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
index 60b546a..c32657d 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml
+++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml
@@ -1,7 +1,9 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: custom_dumpsoftwareversions
 description: Custom module used to dump software versions within the nf-core pipeline template
 keywords:
   - custom
+  - dump
   - version
 tools:
   - custom:
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 68f66be..4b60474 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -1,10 +1,10 @@
 process MULTIQC {
     label 'process_single'
 
-    conda "bioconda::multiqc=1.13"
+    conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
+        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index ebc29b2..f93b5ee 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,3 +1,4 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: MultiQC
 description: Aggregate results from bioinformatics analyses across many samples into a single report
 keywords:
@@ -37,7 +38,7 @@ output:
       description: MultiQC report file
       pattern: "multiqc_report.html"
   - data:
-      type: dir
+      type: directory
       description: MultiQC data dir
       pattern: "multiqc_data"
   - plots:
diff --git a/nextflow.config b/nextflow.config
index 6d815bd..883c216 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -73,7 +73,11 @@ try {
 
 
 profiles {
-    debug { process.beforeScript = 'echo $HOSTNAME' }
+    debug {
+        dumpHashes             = true
+        process.beforeScript   = 'echo $HOSTNAME'
+        cleanup = false
+    }
     conda {
         conda.enabled          = true
         docker.enabled         = false
@@ -81,6 +85,7 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     mamba {
         conda.enabled          = true
@@ -90,14 +95,18 @@ profiles {
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     docker {
         docker.enabled         = true
+        docker.registry        = 'quay.io'
         docker.userEmulation   = true
+        conda.enabled          = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     arm {
         docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64'
@@ -105,31 +114,49 @@ profiles {
     singularity {
         singularity.enabled    = true
         singularity.autoMounts = true
+        conda.enabled          = false
         docker.enabled         = false
         podman.enabled         = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     podman {
         podman.enabled         = true
+        podman.registry        = 'quay.io'
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         shifter.enabled        = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     shifter {
         shifter.enabled        = true
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         podman.enabled         = false
         charliecloud.enabled   = false
+        apptainer.enabled      = false
     }
     charliecloud {
         charliecloud.enabled   = true
+        conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
         podman.enabled         = false
         shifter.enabled        = false
+        apptainer.enabled      = false
+    }
+    apptainer {
+        apptainer.enabled      = true
+        conda.enabled          = false
+        docker.enabled         = false
+        singularity.enabled    = false
+        podman.enabled         = false
+        shifter.enabled        = false
+        charliecloud.enabled   = false
     }
     gitpod {
         executor.name          = 'local'
diff --git a/pipeline_template.yml b/pipeline_template.yml
new file mode 100644
index 0000000..09d0664
--- /dev/null
+++ b/pipeline_template.yml
@@ -0,0 +1,3 @@
+prefix: sanger-tol
+skip:
+- igenomes
diff --git a/tower.yml b/tower.yml
new file mode 100644
index 0000000..787aedf
--- /dev/null
+++ b/tower.yml
@@ -0,0 +1,5 @@
+reports:
+  multiqc_report.html:
+    display: "MultiQC HTML report"
+  samplesheet.csv:
+    display: "Auto-created samplesheet with collated metadata and FASTQ paths"

From 23f24b78983e940dfea350561f40f93eabed394b Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 11:29:16 +0100
Subject: [PATCH 38/85] Fixed the modules installation

---
 modules.json                                  | 68 ++++++++++++-------
 .../nf-core-pipeline => local}/repeats_bed.nf |  0
 .../custom/dumpsoftwareversions/main.nf       |  2 +-
 .../templates/dumpsoftwareversions.py         |  1 +
 .../custom/getchromsizes/main.nf              |  6 +-
 .../custom/getchromsizes/meta.yml             |  0
 .../{modules => }/samtools/dict/main.nf       |  6 +-
 .../{modules => }/samtools/dict/meta.yml      |  0
 .../{modules => }/samtools/faidx/main.nf      | 22 +++---
 .../{modules => }/samtools/faidx/meta.yml     | 14 +++-
 .../nf-core/{modules => }/tabix/bgzip/main.nf | 26 +++++--
 .../{modules => }/tabix/bgzip/meta.yml        |  1 +
 .../nf-core/{modules => }/tabix/tabix/main.nf |  4 +-
 .../{modules => }/tabix/tabix/meta.yml        |  0
 14 files changed, 101 insertions(+), 49 deletions(-)
 rename modules/{sanger-tol/nf-core-pipeline => local}/repeats_bed.nf (100%)
 rename modules/nf-core/{modules => }/custom/getchromsizes/main.nf (88%)
 rename modules/nf-core/{modules => }/custom/getchromsizes/meta.yml (100%)
 rename modules/nf-core/{modules => }/samtools/dict/main.nf (86%)
 rename modules/nf-core/{modules => }/samtools/dict/meta.yml (100%)
 rename modules/nf-core/{modules => }/samtools/faidx/main.nf (59%)
 rename modules/nf-core/{modules => }/samtools/faidx/meta.yml (79%)
 rename modules/nf-core/{modules => }/tabix/bgzip/main.nf (65%)
 rename modules/nf-core/{modules => }/tabix/bgzip/meta.yml (98%)
 rename modules/nf-core/{modules => }/tabix/tabix/main.nf (89%)
 rename modules/nf-core/{modules => }/tabix/tabix/meta.yml (100%)

diff --git a/modules.json b/modules.json
index b6ef8d4..738eadc 100644
--- a/modules.json
+++ b/modules.json
@@ -4,31 +4,51 @@
     "repos": {
         "https://github.com/nf-core/modules.git": {
             "modules": {
-                "custom/dumpsoftwareversions": {
-                    "branch": "master",
-                    "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543"
-                },
-                "custom/getchromsizes": {
-                    "branch": "master",
-                    "git_sha": "d75b37fef175f241230ee25c485bd574c768e282"
-                },
-                "samtools/dict": {
-                    "branch": "master",
-                    "git_sha": "31409f5e727ec932f0b3a399c7a3847d70b21374"
-                },
-                "samtools/faidx": {
-                    "branch": "master",
-                    "git_sha": "3eb99152cedbb7280258858e5df08478a4670696"
-                },
-                "tabix/bgzip": {
-                    "branch": "master",
-                    "git_sha": "31c0b49f6527ef196e89eca49a36af2de71711f8"
-                },
-                "tabix/tabix": {
-                    "branch": "master",
-                    "git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247"
+                "nf-core": {
+                    "custom/dumpsoftwareversions": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "custom/getchromsizes": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/dict": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "samtools/faidx": {
+                        "branch": "master",
+                        "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/bgzip": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "tabix/tabix": {
+                        "branch": "master",
+                        "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
+                        "installed_by": [
+                            "modules"
+                        ]
+                    }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/sanger-tol/nf-core-pipeline/repeats_bed.nf b/modules/local/repeats_bed.nf
similarity index 100%
rename from modules/sanger-tol/nf-core-pipeline/repeats_bed.nf
rename to modules/local/repeats_bed.nf
diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf
index 800a609..ebc8727 100644
--- a/modules/nf-core/custom/dumpsoftwareversions/main.nf
+++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf
@@ -5,7 +5,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS {
     conda "bioconda::multiqc=1.14"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' :
-        'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }"
+        'biocontainers/multiqc:1.14--pyhdfd78af_0' }"
 
     input:
     path versions
diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
index b10e0a5..da03340 100755
--- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
+++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py
@@ -4,6 +4,7 @@
 """Provide functions to merge multiple versions.yml files."""
 
 
+import yaml
 import platform
 from textwrap import dedent
 
diff --git a/modules/nf-core/modules/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf
similarity index 88%
rename from modules/nf-core/modules/custom/getchromsizes/main.nf
rename to modules/nf-core/custom/getchromsizes/main.nf
index 8e1693d..060a2e8 100644
--- a/modules/nf-core/modules/custom/getchromsizes/main.nf
+++ b/modules/nf-core/custom/getchromsizes/main.nf
@@ -2,10 +2,10 @@ process CUSTOM_GETCHROMSIZES {
     tag "$fasta"
     label 'process_single'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    conda "bioconda::samtools=1.16.1"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' :
+        'biocontainers/samtools:1.16.1--h6899075_1' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/modules/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml
similarity index 100%
rename from modules/nf-core/modules/custom/getchromsizes/meta.yml
rename to modules/nf-core/custom/getchromsizes/meta.yml
diff --git a/modules/nf-core/modules/samtools/dict/main.nf b/modules/nf-core/samtools/dict/main.nf
similarity index 86%
rename from modules/nf-core/modules/samtools/dict/main.nf
rename to modules/nf-core/samtools/dict/main.nf
index 91f782b..f5b469b 100644
--- a/modules/nf-core/modules/samtools/dict/main.nf
+++ b/modules/nf-core/samtools/dict/main.nf
@@ -2,10 +2,10 @@ process SAMTOOLS_DICT {
     tag "$fasta"
     label 'process_single'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/modules/samtools/dict/meta.yml b/modules/nf-core/samtools/dict/meta.yml
similarity index 100%
rename from modules/nf-core/modules/samtools/dict/meta.yml
rename to modules/nf-core/samtools/dict/meta.yml
diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
similarity index 59%
rename from modules/nf-core/modules/samtools/faidx/main.nf
rename to modules/nf-core/samtools/faidx/main.nf
index ef940db..59ed308 100644
--- a/modules/nf-core/modules/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -2,18 +2,20 @@ process SAMTOOLS_FAIDX {
     tag "$fasta"
     label 'process_single'
 
-    conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
+    conda "bioconda::samtools=1.17"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
-        'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
+        'biocontainers/samtools:1.17--h00cdaf9_0' }"
 
     input:
     tuple val(meta), path(fasta)
+    tuple val(meta2), path(fai)
 
     output:
-    tuple val(meta), path ("*.fai"), emit: fai
-    tuple val(meta), path ("*.gzi"), emit: gzi, optional: true
-    path "versions.yml"            , emit: versions
+    tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true
+    tuple val(meta), path ("*.fai")        , emit: fai, optional: true
+    tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
+    path "versions.yml"                    , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,8 +25,8 @@ process SAMTOOLS_FAIDX {
     """
     samtools \\
         faidx \\
-        $args \\
-        $fasta
+        $fasta \\
+        $args
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -33,8 +35,12 @@ process SAMTOOLS_FAIDX {
     """
 
     stub:
+    def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll()
+    def fastacmd = match[0] ? "touch ${match[0][1]}" : ''
     """
+    ${fastacmd}
     touch ${fasta}.fai
+
     cat <<-END_VERSIONS > versions.yml
 
     "${task.process}":
diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
similarity index 79%
rename from modules/nf-core/modules/samtools/faidx/meta.yml
rename to modules/nf-core/samtools/faidx/meta.yml
index fe2fe9a..957b25e 100644
--- a/modules/nf-core/modules/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -3,6 +3,7 @@ description: Index FASTA file
 keywords:
   - index
   - fasta
+  - faidx
 tools:
   - samtools:
       description: |
@@ -17,12 +18,21 @@ input:
   - meta:
       type: map
       description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
   - fasta:
       type: file
       description: FASTA file
       pattern: "*.{fa,fasta}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - fai:
+      type: file
+      description: FASTA index file
+      pattern: "*.{fai}"
 output:
   - meta:
       type: map
diff --git a/modules/nf-core/modules/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf
similarity index 65%
rename from modules/nf-core/modules/tabix/bgzip/main.nf
rename to modules/nf-core/tabix/bgzip/main.nf
index aaef785..8c47d9e 100644
--- a/modules/nf-core/modules/tabix/bgzip/main.nf
+++ b/modules/nf-core/tabix/bgzip/main.nf
@@ -2,10 +2,10 @@ process TABIX_BGZIP {
     tag "$meta.id"
     label 'process_single'
 
-    conda (params.enable_conda ? 'bioconda::tabix=1.11' : null)
+    conda "bioconda::tabix=1.11"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }"
+        'biocontainers/tabix:1.11--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(input)
@@ -22,15 +22,29 @@ process TABIX_BGZIP {
     def args = task.ext.args ?: ''
     prefix   = task.ext.prefix ?: "${meta.id}"
     in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension())
-    output   = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz"
-    command1 = in_bgzip ? '-d' : '-c'
-    command2 = in_bgzip ? ''   : " > ${output}"
+    extension = in_bgzip ? input.getBaseName().tokenize(".")[-1] : input.getExtension()
+    output   = in_bgzip ? "${prefix}.${extension}" : "${prefix}.${extension}.gz"
+    command = in_bgzip ? '-d' : ''
     // Name the index according to $prefix, unless a name has been requested
     if ((args.matches("(^| )-i\\b") || args.matches("(^| )--index(\$| )")) && !args.matches("(^| )-I\\b") && !args.matches("(^| )--index-name\\b")) {
         args = args + " -I ${output}.gzi"
     }
     """
-    bgzip $command1 $args -@${task.cpus} $input $command2
+    bgzip $command -c $args -@${task.cpus} $input > ${output}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    prefix   = task.ext.prefix ?: "${meta.id}"
+    in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension())
+    output   = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz"
+
+    """
+    touch ${output}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/modules/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml
similarity index 98%
rename from modules/nf-core/modules/tabix/bgzip/meta.yml
rename to modules/nf-core/tabix/bgzip/meta.yml
index 72f0abc..c3ea210 100644
--- a/modules/nf-core/modules/tabix/bgzip/meta.yml
+++ b/modules/nf-core/tabix/bgzip/meta.yml
@@ -44,3 +44,4 @@ authors:
   - "@joseespinosa"
   - "@drpatelh"
   - "@maxulysse"
+  - "@nvnieuwk"
diff --git a/modules/nf-core/modules/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf
similarity index 89%
rename from modules/nf-core/modules/tabix/tabix/main.nf
rename to modules/nf-core/tabix/tabix/main.nf
index 21b2e79..5bf332e 100644
--- a/modules/nf-core/modules/tabix/tabix/main.nf
+++ b/modules/nf-core/tabix/tabix/main.nf
@@ -2,10 +2,10 @@ process TABIX_TABIX {
     tag "$meta.id"
     label 'process_single'
 
-    conda (params.enable_conda ? 'bioconda::tabix=1.11' : null)
+    conda "bioconda::tabix=1.11"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' :
-        'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }"
+        'biocontainers/tabix:1.11--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(tab)
diff --git a/modules/nf-core/modules/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml
similarity index 100%
rename from modules/nf-core/modules/tabix/tabix/meta.yml
rename to modules/nf-core/tabix/tabix/meta.yml

From bfce31fb9e0ad1c03c7dd3b4caea37ba36ef2722 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 11:29:52 +0100
Subject: [PATCH 39/85] [linting] prettier

---
 .github/ISSUE_TEMPLATE/bug_report.yml | 100 +++++++++++++-------------
 CITATION.cff                          |  24 +++----
 README.md                             |   6 +-
 modules.json                          |  26 ++-----
 nextflow_schema.json                  |   9 +--
 pipeline_template.yml                 |   2 +-
 6 files changed, 74 insertions(+), 93 deletions(-)

diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 06441d4..ffd6c7c 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -2,53 +2,53 @@ name: Bug report
 description: Report something that is broken or incorrect
 labels: bug
 body:
-- type: textarea
-  id: description
-  attributes:
-    label: Description of the bug
-    description: A clear and concise description of what the bug is.
-  validations:
-    required: true
-- type: textarea
-  id: command_used
-  attributes:
-    label: Command used and terminal output
-    description: Steps to reproduce the behaviour. Please paste the command you used
-      to launch the pipeline and the output from your terminal.
-    render: console
-    placeholder: '$ nextflow run ...
-
-
-      Some output where something broke
-
-      '
-- type: textarea
-  id: files
-  attributes:
-    label: Relevant files
-    description: 'Please drag and drop the relevant files here. Create a `.zip` archive
-      if the extension is not allowed.
-
-      Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
-      in the directory where you launched the pipeline)_ as well as custom Nextflow
-      configuration files.
-
-      '
-- type: textarea
-  id: system
-  attributes:
-    label: System information
-    description: '* Nextflow version _(eg. 22.10.1)_
-
-      * Hardware _(eg. HPC, Desktop, Cloud)_
-
-      * Executor _(eg. slurm, local, awsbatch)_
-
-      * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud,
-      or Apptainer)_
-
-      * OS _(eg. CentOS Linux, macOS, Linux Mint)_
-
-      * Version of sanger-tol/insdcdownload _(eg. 1.1, 1.5, 1.8.2)_
-
-      '
+  - type: textarea
+    id: description
+    attributes:
+      label: Description of the bug
+      description: A clear and concise description of what the bug is.
+    validations:
+      required: true
+  - type: textarea
+    id: command_used
+    attributes:
+      label: Command used and terminal output
+      description: Steps to reproduce the behaviour. Please paste the command you used
+        to launch the pipeline and the output from your terminal.
+      render: console
+      placeholder: "$ nextflow run ...
+
+
+        Some output where something broke
+
+        "
+  - type: textarea
+    id: files
+    attributes:
+      label: Relevant files
+      description: "Please drag and drop the relevant files here. Create a `.zip` archive
+        if the extension is not allowed.
+
+        Your verbose log file `.nextflow.log` is often useful _(this is a hidden file
+        in the directory where you launched the pipeline)_ as well as custom Nextflow
+        configuration files.
+
+        "
+  - type: textarea
+    id: system
+    attributes:
+      label: System information
+      description: "* Nextflow version _(eg. 22.10.1)_
+
+        * Hardware _(eg. HPC, Desktop, Cloud)_
+
+        * Executor _(eg. slurm, local, awsbatch)_
+
+        * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud,
+        or Apptainer)_
+
+        * OS _(eg. CentOS Linux, macOS, Linux Mint)_
+
+        * Version of sanger-tol/insdcdownload _(eg. 1.1, 1.5, 1.8.2)_
+
+        "
diff --git a/CITATION.cff b/CITATION.cff
index 5e7a3c3..b7b4573 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -4,21 +4,21 @@
 cff-version: 1.2.0
 title: sanger-tol/insdcdownload v1.1.0 - Deciduous ent
 message: >-
-  If you use this software, please cite it using the
-  metadata from this file.
+    If you use this software, please cite it using the
+    metadata from this file.
 type: software
 authors:
-  - given-names: Matthieu
-    family-names: Muffato
-    affiliation: Wellcome Sanger Institute
-    orcid: "https://orcid.org/0000-0002-7860-3560"
-  - given-names: Priyanka
-    family-names: Surana
-    orcid: "https://orcid.org/0000-0002-7167-0875"
-    affiliation: Wellcome Sanger Institute
+    - given-names: Matthieu
+      family-names: Muffato
+      affiliation: Wellcome Sanger Institute
+      orcid: "https://orcid.org/0000-0002-7860-3560"
+    - given-names: Priyanka
+      family-names: Surana
+      orcid: "https://orcid.org/0000-0002-7167-0875"
+      affiliation: Wellcome Sanger Institute
 identifiers:
-  - type: doi
-    value: 10.5281/zenodo.7155119
+    - type: doi
+      value: 10.5281/zenodo.7155119
 repository-code: "https://github.com/sanger-tol/insdcdownload"
 license: MIT
 commit: TODO
diff --git a/README.md b/README.md
index 0daff90..21eae34 100644
--- a/README.md
+++ b/README.md
@@ -48,9 +48,9 @@ Steps involved:
 > to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
 > with `-profile test` before running the workflow on actual data.
 
-   ```console
-   nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir results
-   ```
+```console
+nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir results
+```
 
 > **Warning:**
 > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those
diff --git a/modules.json b/modules.json
index 738eadc..b3f059d 100644
--- a/modules.json
+++ b/modules.json
@@ -8,47 +8,35 @@
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/dict": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7c22167..4af1a8a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -165,14 +165,7 @@
                     "description": "Method used to save pipeline results to output directory.",
                     "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
                     "fa_icon": "fas fa-copy",
-                    "enum": [
-                        "symlink",
-                        "rellink",
-                        "link",
-                        "copy",
-                        "copyNoFollow",
-                        "move"
-                    ],
+                    "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
                     "hidden": true
                 },
                 "email_on_fail": {
diff --git a/pipeline_template.yml b/pipeline_template.yml
index 09d0664..0aa7398 100644
--- a/pipeline_template.yml
+++ b/pipeline_template.yml
@@ -1,3 +1,3 @@
 prefix: sanger-tol
 skip:
-- igenomes
+  - igenomes

From 31e5f8c2b540deafa3487f331eff75c2b35b6ef0 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 11:30:11 +0100
Subject: [PATCH 40/85] [linting] black

---
 bin/check_samplesheet.py | 4 +---
 bin/repeats_bed.py       | 2 --
 2 files changed, 1 insertion(+), 5 deletions(-)

diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index a8acaa1..de6852e 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -78,9 +78,7 @@ def _validate_accession(self, row):
         if not row[self._accession_col]:
             raise AssertionError("Accession number is required.")
         if not self._regex_accession.match(row[self._accession_col]):
-            raise AssertionError(
-                "Accession numbers must match %s." % self._regex_accession
-            )
+            raise AssertionError("Accession numbers must match %s." % self._regex_accession)
 
     def _validate_name(self, row):
         """Assert that the assembly name is non-empty and has no space."""
diff --git a/bin/repeats_bed.py b/bin/repeats_bed.py
index d4ff4b7..e4bd8cd 100755
--- a/bin/repeats_bed.py
+++ b/bin/repeats_bed.py
@@ -9,7 +9,6 @@
 
 
 def fasta_to_bed(fasta):
-
     in_gap = None
     with gzip.open(fasta, "rt") if fasta.endswith(".gz") else open(fasta) as fh:
         for line in fh:
@@ -39,7 +38,6 @@ def fasta_to_bed(fasta):
 
 
 if __name__ == "__main__":
-
     parser = argparse.ArgumentParser(description=__doc__)
     parser.add_argument("fasta", help="Input Fasta file.")
     parser.add_argument("--version", action="version", version="%(prog)s 1.1")

From 9268ef6b605150fd9eeb750f678b1fd27afade06 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 14 Jul 2023 12:12:54 +0100
Subject: [PATCH 41/85] Fixed the modules path

---
 subworkflows/sanger-tol/prepare_fasta.nf   | 6 +++---
 subworkflows/sanger-tol/prepare_repeats.nf | 8 ++++----
 workflows/insdcdownload.nf                 | 2 +-
 3 files changed, 8 insertions(+), 8 deletions(-)

diff --git a/subworkflows/sanger-tol/prepare_fasta.nf b/subworkflows/sanger-tol/prepare_fasta.nf
index 13b6859..c615e63 100644
--- a/subworkflows/sanger-tol/prepare_fasta.nf
+++ b/subworkflows/sanger-tol/prepare_fasta.nf
@@ -2,9 +2,9 @@
 // Prepare all the indexes for a Fasta file
 //
 
-include { CUSTOM_GETCHROMSIZES    } from '../../modules/nf-core/modules/custom/getchromsizes/main'
-include { SAMTOOLS_DICT           } from '../../modules/nf-core/modules/samtools/dict/main'
-include { TABIX_BGZIP             } from '../../modules/nf-core/modules/tabix/bgzip/main'
+include { CUSTOM_GETCHROMSIZES    } from '../../modules/nf-core/custom/getchromsizes/main'
+include { SAMTOOLS_DICT           } from '../../modules/nf-core/samtools/dict/main'
+include { TABIX_BGZIP             } from '../../modules/nf-core/tabix/bgzip/main'
 
 
 workflow PREPARE_FASTA {
diff --git a/subworkflows/sanger-tol/prepare_repeats.nf b/subworkflows/sanger-tol/prepare_repeats.nf
index 64d23f1..df42c52 100644
--- a/subworkflows/sanger-tol/prepare_repeats.nf
+++ b/subworkflows/sanger-tol/prepare_repeats.nf
@@ -3,10 +3,10 @@
 // and prepare indexes for it
 //
 
-include { REPEATS_BED                      } from '../../modules/sanger-tol/nf-core-pipeline/repeats_bed'
-include { TABIX_BGZIP                      } from '../../modules/nf-core/modules/tabix/bgzip/main'
-include { TABIX_TABIX as TABIX_TABIX_CSI   } from '../../modules/nf-core/modules/tabix/tabix/main'
-include { TABIX_TABIX as TABIX_TABIX_TBI   } from '../../modules/nf-core/modules/tabix/tabix/main'
+include { REPEATS_BED                      } from '../../modules/local/repeats_bed'
+include { TABIX_BGZIP                      } from '../../modules/nf-core/tabix/bgzip/main'
+include { TABIX_TABIX as TABIX_TABIX_CSI   } from '../../modules/nf-core/tabix/tabix/main'
+include { TABIX_TABIX as TABIX_TABIX_TBI   } from '../../modules/nf-core/tabix/tabix/main'
 
 
 workflow PREPARE_REPEATS {
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index 67d4b20..3974a30 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -33,7 +33,7 @@ include { PREPARE_REPEATS                              } from '../subworkflows/s
 //
 // MODULE: Installed directly from nf-core/modules
 //
-include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main'
+include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From 2d86b293cb5d565cd75d5789e8711ce393a7ba90 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:09:21 +0100
Subject: [PATCH 42/85] Now need nf-core 2.8

---
 .github/workflows/linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a19f141..a5be907 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -84,7 +84,7 @@ jobs:
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core==2.5.0
+          pip install nf-core==2.8
 
       - name: Run nf-core lint
         env:

From f9dadd032d0774006fcea45acc9abb0e27cdec81 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:09:59 +0100
Subject: [PATCH 43/85] we use prettier for the cff files

---
 .github/workflows/linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a5be907..7ebc310 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -22,7 +22,7 @@ jobs:
         run: npm install -g editorconfig-checker
 
       - name: Run ECLint check
-        run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile')
+        run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|cff\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile')
 
   Prettier:
     runs-on: ubuntu-latest

From cb4d091637295c95f48fccad6b3c671570e86fd1 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:24:34 +0100
Subject: [PATCH 44/85] Need to use Nextflow.error

---
 lib/WorkflowInsdcdownload.groovy | 10 +++-------
 1 file changed, 3 insertions(+), 7 deletions(-)

diff --git a/lib/WorkflowInsdcdownload.groovy b/lib/WorkflowInsdcdownload.groovy
index 1a5934c..d536dde 100755
--- a/lib/WorkflowInsdcdownload.groovy
+++ b/lib/WorkflowInsdcdownload.groovy
@@ -3,7 +3,6 @@
 //
 
 import nextflow.Nextflow
-import groovy.text.SimpleTemplateEngine
 
 class WorkflowInsdcdownload {
 
@@ -16,18 +15,15 @@ class WorkflowInsdcdownload {
         if (params.input) {
             def f = new File(params.input);
             if (!f.exists()) {
-                log.error "'${params.input}' doesn't exist"
-                System.exit(1)
+                Nextflow.error "'${params.input}' doesn't exist"
             }
         } else {
             if (!params.assembly_accession || !params.assembly_name) {
-                log.error "Either --input, or --assembly_accession and --assembly_name must be provided"
-                System.exit(1)
+                Nextflow.error "Either --input, or --assembly_accession and --assembly_name must be provided"
             }
         }
         if (!params.outdir) {
-            log.error "--outdir is mandatory"
-            System.exit(1)
+            Nextflow.error "--outdir is mandatory"
         }
     }
 

From 4540aa1cb7464a9144b36270ef46b609bd48c453 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:25:25 +0100
Subject: [PATCH 45/85] [linting] prettier

---
 modules.json | 26 +++++++++++++++++++-------
 1 file changed, 19 insertions(+), 7 deletions(-)

diff --git a/modules.json b/modules.json
index b3f059d..738eadc 100644
--- a/modules.json
+++ b/modules.json
@@ -8,35 +8,47 @@
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/dict": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file

From 931c24314dcf9ee481742274f450361087a2b668 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:33:09 +0100
Subject: [PATCH 46/85] Fixed the Conda directives

---
 modules/local/ncbi_download.nf  | 2 +-
 modules/local/remove_masking.nf | 2 +-
 modules/local/repeats_bed.nf    | 2 +-
 3 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/ncbi_download.nf b/modules/local/ncbi_download.nf
index dc57a21..9c18357 100644
--- a/modules/local/ncbi_download.nf
+++ b/modules/local/ncbi_download.nf
@@ -6,7 +6,7 @@ process NCBI_DOWNLOAD {
     tag "$assembly_accession"
     label 'process_single'
 
-    conda (params.enable_conda ? "bioconda::wget=1.18" : null)
+    conda "bioconda::wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h7132678_6' :
         'quay.io/biocontainers/gnu-wget:1.18--h7132678_6' }"
diff --git a/modules/local/remove_masking.nf b/modules/local/remove_masking.nf
index b5d2cf3..fc3974b 100644
--- a/modules/local/remove_masking.nf
+++ b/modules/local/remove_masking.nf
@@ -3,7 +3,7 @@ process REMOVE_MASKING {
     tag "$genome"
     label 'process_single'
 
-    conda (params.enable_conda ? "conda-forge::gawk=5.1.0" : null)
+    conda "conda-forge::gawk=5.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gawk:5.1.0' :
         'quay.io/biocontainers/gawk:5.1.0' }"
diff --git a/modules/local/repeats_bed.nf b/modules/local/repeats_bed.nf
index 8f66714..205c59e 100644
--- a/modules/local/repeats_bed.nf
+++ b/modules/local/repeats_bed.nf
@@ -4,7 +4,7 @@ process REPEATS_BED {
     tag "$genome"
     label 'process_single'
 
-    conda (params.enable_conda ? "conda-forge::python=3.9.1" : null)
+    conda "conda-forge::python=3.9.1"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.9--1' :
         'quay.io/biocontainers/python:3.9--1' }"

From 435fcf1d7168f5ba944d4264d798c88b9e153179 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 10:33:25 +0100
Subject: [PATCH 47/85] The quay.io prefix should now be skipped

---
 modules/local/ncbi_download.nf  | 2 +-
 modules/local/remove_masking.nf | 2 +-
 modules/local/repeats_bed.nf    | 2 +-
 3 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/ncbi_download.nf b/modules/local/ncbi_download.nf
index 9c18357..ddcc70e 100644
--- a/modules/local/ncbi_download.nf
+++ b/modules/local/ncbi_download.nf
@@ -9,7 +9,7 @@ process NCBI_DOWNLOAD {
     conda "bioconda::wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h7132678_6' :
-        'quay.io/biocontainers/gnu-wget:1.18--h7132678_6' }"
+        'biocontainers/gnu-wget:1.18--h7132678_6' }"
 
     input:
     tuple val(assembly_accession), val(assembly_name), val(species_dir)
diff --git a/modules/local/remove_masking.nf b/modules/local/remove_masking.nf
index fc3974b..7a865f7 100644
--- a/modules/local/remove_masking.nf
+++ b/modules/local/remove_masking.nf
@@ -6,7 +6,7 @@ process REMOVE_MASKING {
     conda "conda-forge::gawk=5.1.0"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gawk:5.1.0' :
-        'quay.io/biocontainers/gawk:5.1.0' }"
+        'biocontainers/gawk:5.1.0' }"
 
     input:
     tuple val(meta), path(genome)
diff --git a/modules/local/repeats_bed.nf b/modules/local/repeats_bed.nf
index 205c59e..4a7c166 100644
--- a/modules/local/repeats_bed.nf
+++ b/modules/local/repeats_bed.nf
@@ -7,7 +7,7 @@ process REPEATS_BED {
     conda "conda-forge::python=3.9.1"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.9--1' :
-        'quay.io/biocontainers/python:3.9--1' }"
+        'biocontainers/python:3.9--1' }"
 
     input:
     tuple val(meta), path(genome)

From a9551d58ede70d4a8f1f7f624fb3653ab710a15c Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 14:07:12 +0100
Subject: [PATCH 48/85] No output for Tower

---
 tower.yml | 5 -----
 1 file changed, 5 deletions(-)
 delete mode 100644 tower.yml

diff --git a/tower.yml b/tower.yml
deleted file mode 100644
index 787aedf..0000000
--- a/tower.yml
+++ /dev/null
@@ -1,5 +0,0 @@
-reports:
-  multiqc_report.html:
-    display: "MultiQC HTML report"
-  samplesheet.csv:
-    display: "Auto-created samplesheet with collated metadata and FASTQ paths"

From 1d2524a881e2ae3c798745c51f5f1c4268ea9062 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 14:07:30 +0100
Subject: [PATCH 49/85] We don't use MultiQC

---
 assets/methods_description_template.yml | 25 -------------------------
 1 file changed, 25 deletions(-)
 delete mode 100644 assets/methods_description_template.yml

diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml
deleted file mode 100644
index 76b0c0e..0000000
--- a/assets/methods_description_template.yml
+++ /dev/null
@@ -1,25 +0,0 @@
-id: "sanger-tol-insdcdownload-methods-description"
-description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication."
-section_name: "sanger-tol/insdcdownload Methods Description"
-section_href: "https://github.com/sanger-tol/insdcdownload"
-plot_type: "html"
-## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline
-## You inject any metadata in the Nextflow '${workflow}' object
-data: |
-  <h4>Methods</h4>
-  <p>Data was processed using sanger-tol/insdcdownload v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>).</p>
-  <p>The pipeline was executed with Nextflow v${workflow.nextflow.version} (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p>
-  <pre><code>${workflow.commandLine}</code></pre>
-  <h4>References</h4>
-  <ul>
-    <li>Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. <a href="https://doi.org/10.1038/nbt.3820">https://doi.org/10.1038/nbt.3820</a></li>
-    <li>Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. <a href="https://doi.org/10.1038/s41587-020-0439-x">https://doi.org/10.1038/s41587-020-0439-x</a></li>
-  </ul>
-  <div class="alert alert-info">
-    <h5>Notes:</h5>
-    <ul>
-      ${nodoi_text}
-      <li>The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!</li>
-      <li>You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.</li>
-    </ul>
-  </div>

From 7254f04f88c014434377d660d1b9c8173c3499a2 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 14:07:46 +0100
Subject: [PATCH 50/85] Fixed the wget conda package

---
 modules/local/ncbi_download.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/ncbi_download.nf b/modules/local/ncbi_download.nf
index ddcc70e..54196d2 100644
--- a/modules/local/ncbi_download.nf
+++ b/modules/local/ncbi_download.nf
@@ -6,7 +6,7 @@ process NCBI_DOWNLOAD {
     tag "$assembly_accession"
     label 'process_single'
 
-    conda "bioconda::wget=1.18"
+    conda "bioconda::gnu-wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h7132678_6' :
         'biocontainers/gnu-wget:1.18--h7132678_6' }"

From 890dbb6a490582eace424030f3a5956394f1cf33 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 14:11:11 +0100
Subject: [PATCH 51/85] [linting] prettier

---
 modules.json | 26 +++++++-------------------
 1 file changed, 7 insertions(+), 19 deletions(-)

diff --git a/modules.json b/modules.json
index 738eadc..b3f059d 100644
--- a/modules.json
+++ b/modules.json
@@ -8,47 +8,35 @@
                     "custom/dumpsoftwareversions": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "custom/getchromsizes": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/dict": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "fd742419940e01ba1c5ecb172c3e32ec840662fe",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/bgzip": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "tabix/tabix": {
                         "branch": "master",
                         "git_sha": "911696ea0b62df80e900ef244d7867d177971f73",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From e903d2510646c6744d9c0a73c670b4bed4828813 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 21:56:24 +0100
Subject: [PATCH 52/85] was 2 before the template update

---
 conf/base.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/base.config b/conf/base.config
index bdbed0f..316a11d 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -7,7 +7,7 @@
 process {
 
     errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
-    maxRetries    = 1
+    maxRetries    = 2
     maxErrors     = '-1'
 
     // Most of the pipeline requires very little resources

From 81178e5a1a9e2d222d7a3c6556a9872740a84812 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 22:01:59 +0100
Subject: [PATCH 53/85] We do this through a profile

---
 conf/test_full.config | 2 --
 1 file changed, 2 deletions(-)

diff --git a/conf/test_full.config b/conf/test_full.config
index 03c7af7..e639d86 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -10,8 +10,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-cleanup = true
-
 params {
     config_profile_name        = 'Full test profile'
     config_profile_description = 'Full test dataset to check pipeline function'

From b540ef3ca41240f903e8b984d26d264f41b3b695 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 22:07:38 +0100
Subject: [PATCH 54/85] Fixed the example params.yaml

---
 docs/usage.md | 6 ++----
 1 file changed, 2 insertions(+), 4 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 46dd575..c3cfbb8 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -80,11 +80,9 @@ nextflow run sanger-tol/insdcdownload -profile docker -params-file params.yaml
 with `params.yaml` containing:
 
 ```yaml
-input: './samplesheet.csv'
+assembly: 'gfLaeSulp1.1'
+assembly_accession: 'GCA_927399515.1'
 outdir: './results/'
-genome: 'GRCh37'
-input: 'data'
-<...>
 ```
 
 You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).

From 9682670ea6d29a8e7000c120eb3e5258dac655d5 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 22:09:36 +0100
Subject: [PATCH 55/85] [linting] prettier

---
 docs/usage.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index c3cfbb8..0f25713 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -80,9 +80,9 @@ nextflow run sanger-tol/insdcdownload -profile docker -params-file params.yaml
 with `params.yaml` containing:
 
 ```yaml
-assembly: 'gfLaeSulp1.1'
-assembly_accession: 'GCA_927399515.1'
-outdir: './results/'
+assembly: "gfLaeSulp1.1"
+assembly_accession: "GCA_927399515.1"
+outdir: "./results/"
 ```
 
 You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).

From d7951cfe95a3a298eb5aeaa9e875d7d0eaf67742 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 22:22:32 +0100
Subject: [PATCH 56/85] We don't make shared sanger-tol workflows anymore

---
 .gitattributes                                        | 2 --
 subworkflows/{sanger-tol => local}/prepare_fasta.nf   | 0
 subworkflows/{sanger-tol => local}/prepare_repeats.nf | 0
 workflows/insdcdownload.nf                            | 6 +++---
 4 files changed, 3 insertions(+), 5 deletions(-)
 rename subworkflows/{sanger-tol => local}/prepare_fasta.nf (100%)
 rename subworkflows/{sanger-tol => local}/prepare_repeats.nf (100%)

diff --git a/.gitattributes b/.gitattributes
index 2d80efd..7a2dabc 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1,6 +1,4 @@
 *.config linguist-language=nextflow
 *.nf.test linguist-language=nextflow
 modules/nf-core/** linguist-generated
-modules/sanger-tol/** linguist-generated
 subworkflows/nf-core/** linguist-generated
-subworkflows/sanger-tol/** linguist-generated
diff --git a/subworkflows/sanger-tol/prepare_fasta.nf b/subworkflows/local/prepare_fasta.nf
similarity index 100%
rename from subworkflows/sanger-tol/prepare_fasta.nf
rename to subworkflows/local/prepare_fasta.nf
diff --git a/subworkflows/sanger-tol/prepare_repeats.nf b/subworkflows/local/prepare_repeats.nf
similarity index 100%
rename from subworkflows/sanger-tol/prepare_repeats.nf
rename to subworkflows/local/prepare_repeats.nf
diff --git a/workflows/insdcdownload.nf b/workflows/insdcdownload.nf
index 3974a30..67793b7 100644
--- a/workflows/insdcdownload.nf
+++ b/workflows/insdcdownload.nf
@@ -20,9 +20,9 @@ WorkflowInsdcdownload.initialise(params, log)
 //
 include { DOWNLOAD_GENOME                              } from '../subworkflows/local/download_genome'
 include { PARAMS_CHECK                                 } from '../subworkflows/local/params_check'
-include { PREPARE_FASTA as PREPARE_UNMASKED_FASTA      } from '../subworkflows/sanger-tol/prepare_fasta'
-include { PREPARE_FASTA as PREPARE_REPEAT_MASKED_FASTA } from '../subworkflows/sanger-tol/prepare_fasta'
-include { PREPARE_REPEATS                              } from '../subworkflows/sanger-tol/prepare_repeats'
+include { PREPARE_FASTA as PREPARE_UNMASKED_FASTA      } from '../subworkflows/local/prepare_fasta'
+include { PREPARE_FASTA as PREPARE_REPEAT_MASKED_FASTA } from '../subworkflows/local/prepare_fasta'
+include { PREPARE_REPEATS                              } from '../subworkflows/local/prepare_repeats'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From f17c7763fbdbae8ca8cf129ba28fe55c3b8c9379 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 22:55:08 +0100
Subject: [PATCH 57/85] Not needed

---
 docs/parameters.md | 67 ----------------------------------------------
 1 file changed, 67 deletions(-)
 delete mode 100644 docs/parameters.md

diff --git a/docs/parameters.md b/docs/parameters.md
deleted file mode 100644
index a4d1569..0000000
--- a/docs/parameters.md
+++ /dev/null
@@ -1,67 +0,0 @@
-# sanger-tol/insdcdownload pipeline parameters
-
-Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure
-
-## Input/output options
-
-Define where the pipeline should find input data and save output data.
-
-| Parameter                                                                                                                                                        | Description                                                                                                                                                                                          | Type     | Default | Required | Hidden |
-| ---------------------------------------------------------------------------------------------------------------------------------------------------------------- | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ |
-| `assembly_accession`                                                                                                                                             | Accession number of the assembly to download. Typically of the form `GCA_*.*`                                                                                                                        | `string` |         |          |        |
-| `assembly_name`                                                                                                                                                  | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.                                                                                                                                   | `string` |         |          |        |
-| `outdir`                                                                                                                                                         | The output directory where the results will be saved. Not considered when running the pipeline with a .csv file as input.                                                                            | `string` |         |          |        |
-| `input`                                                                                                                                                          | Path to comma-separated file containing information about the assemblies to download. Used for bulk download of many assemblies. <details><summary>Help</summary><small>The file has to be a         |
-| comma-separated file with three columns, and a header row. The columns names must be `species_dir`, `assembly_accession`, and `assembly_name`.</small></details> | `string`                                                                                                                                                                                             |          |         |          |
-| `ftp_root`                                                                                                                                                       | Root location of the NCBI FTP, in which all assemblies can be found. Access protocol is actually not limited to FTP, and we use HTTPS by default.                                                    | `string` |
-| https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA                                                                                                                     |                                                                                                                                                                                                      | True     |
-| `email`                                                                                                                                                          | Email address for completion summary. <details><summary>Help</summary><small>Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow |
-| exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.</small></details>           | `string`                                                                                                                                                                                             |          |         | True     |
-
-## Institutional config options
-
-Parameters used to describe centralised config profiles. These should not be edited.
-
-| Parameter                                                                                                                                                                                                  | Description                                                                                                                                                                            | Type     | Default | Required | Hidden |
-| ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- | ------- | -------- | ------ |
-| `custom_config_version`                                                                                                                                                                                    | Git commit id for Institutional configs.                                                                                                                                               | `string` | master  |          | True   |
-| `custom_config_base`                                                                                                                                                                                       | Base directory for Institutional configs. <details><summary>Help</summary><small>If you're running offline, Nextflow will not be able to fetch the institutional config files from the |
-| internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.</small></details> |
-| `string`                                                                                                                                                                                                   | https://raw.githubusercontent.com/nf-core/configs/master                                                                                                                               |          | True    |
-| `config_profile_name`                                                                                                                                                                                      | Institutional config name.                                                                                                                                                             | `string` |         |          | True   |
-| `config_profile_description`                                                                                                                                                                               | Institutional config description.                                                                                                                                                      | `string` |         |          | True   |
-| `config_profile_contact`                                                                                                                                                                                   | Institutional config contact information.                                                                                                                                              | `string` |         |          | True   |
-| `config_profile_url`                                                                                                                                                                                       | Institutional config URL link.                                                                                                                                                         | `string` |         |          | True   |
-
-## Max job request options
-
-Set the top limit for requested resources for any single job.
-
-| Parameter                                                                      | Description                                                                                                                                                                                        | Type   | Default | Required | Hidden |
-| ------------------------------------------------------------------------------ | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------ | ------- | -------- | ------ |
-| `max_cpus`                                                                     | Maximum number of CPUs that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the CPU requirement for each process. Should be an integer   |
-| e.g. `--max_cpus 1`</small></details>                                          | `integer`                                                                                                                                                                                          | 16     |         | True     |
-| `max_memory`                                                                   | Maximum amount of memory that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the memory requirement for each process. Should be a       |
-| string in the format integer-unit e.g. `--max_memory '8.GB'`</small></details> | `string`                                                                                                                                                                                           | 128.GB |         | True     |
-| `max_time`                                                                     | Maximum amount of time that can be requested for any single job. <details><summary>Help</summary><small>Use to set an upper-limit for the time requirement for each process. Should be a string in |
-| the format integer-unit e.g. `--max_time '2.h'`</small></details>              | `string`                                                                                                                                                                                           | 240.h  |         | True     |
-
-## Generic options
-
-Less common options for the pipeline, typically set in a config file.
-
-| Parameter                                                                                                                                                                                                        | Description                                                                                                                                                                                   | Type      | Default                        | Required | Hidden |
-| ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------------------------------ | -------- | ------ |
-| `help`                                                                                                                                                                                                           | Display help text.                                                                                                                                                                            | `boolean` |                                |          | True   |
-| `publish_dir_mode`                                                                                                                                                                                               | Method used to save pipeline results to output directory. <details><summary>Help</summary><small>The Nextflow `publishDir` option specifies which intermediate files should be saved to the   |
-| output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.</small></details> |
-| `string`                                                                                                                                                                                                         | copy                                                                                                                                                                                          |           | True                           |
-| `email_on_fail`                                                                                                                                                                                                  | Email address for completion summary, only when pipeline fails. <details><summary>Help</summary><small>An email address to send a summary email to when the pipeline is completed - ONLY sent |
-| if the pipeline does not exit successfully.</small></details>                                                                                                                                                    | `string`                                                                                                                                                                                      |           |                                | True     |
-| `plaintext_email`                                                                                                                                                                                                | Send plain-text email instead of HTML.                                                                                                                                                        | `boolean` |                                |          | True   |
-| `monochrome_logs`                                                                                                                                                                                                | Do not use coloured log outputs.                                                                                                                                                              | `boolean` |                                |          | True   |
-| `tracedir`                                                                                                                                                                                                       | Directory to keep pipeline Nextflow logs and reports.                                                                                                                                         | `string`  | ${params.outdir}/pipeline_info |          | True   |
-| `validate_params`                                                                                                                                                                                                | Boolean whether to validate parameters against the schema at runtime                                                                                                                          | `boolean` | True                           |          | True   |
-| `show_hidden_params`                                                                                                                                                                                             | Show all params when using `--help` <details><summary>Help</summary><small>By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with       |
-| `--help`. Specifying this option will tell the pipeline to show all parameters.</small></details>                                                                                                                | `boolean`                                                                                                                                                                                     |           |                                | True     |
-| `enable_conda`                                                                                                                                                                                                   | Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.                                                                                          | `boolean` |                                |          | True   |

From d3ade9989b350e0831b08fb6b339d3cbc4c74ff9 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 15 Jul 2023 23:36:12 +0100
Subject: [PATCH 58/85] This file is now identical

---
 .nf-core.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 40826dc..ad4c83b 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -12,7 +12,6 @@ lint:
     - .github/workflows/awsfulltest.yml
   files_unchanged:
     - LICENSE
-    - .gitattributes
     - .github/CONTRIBUTING.md
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - .github/workflows/linting.yml

From 8d9bc1cad1541eac679fbda212e91ecce88cfc40 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 11:58:23 +0100
Subject: [PATCH 59/85] Leftover from a copy

Co-authored-by: Priyanka Surana <ps22@sanger.ac.uk>
---
 assets/slackreport.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/assets/slackreport.json b/assets/slackreport.json
index 043d02f..ea96df5 100644
--- a/assets/slackreport.json
+++ b/assets/slackreport.json
@@ -3,7 +3,7 @@
         {
             "fallback": "Plain-text summary of the attachment.",
             "color": "<% if (success) { %>good<% } else { %>danger<%} %>",
-            "author_name": "sanger-tol/readmapping v${version} - ${runName}",
+            "author_name": "sanger-tol/insdcdownload v${version} - ${runName}",
             "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico",
             "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>",
             "fields": [

From 7b33db6a1d74f2ce0a18b8bf9051deda5fc7cf9f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 11:00:30 +0000
Subject: [PATCH 60/85] Added a redirection notice to the pipeline website

---
 docs/usage.md | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/docs/usage.md b/docs/usage.md
index 0f25713..0b120bc 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -1,5 +1,9 @@
 # sanger-tol/insdcdownload: Usage
 
+## :warning: Please read this documentation on the nf-core website: [https://pipelines.tol.sanger.ac.uk/insdcdownload/usage](https://pipelines.tol.sanger.ac.uk/insdcdownload/usage)
+
+> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._
+
 ## Introduction
 
 The pipeline takes an assembly accession number, as well as the assembly name, and downloads it in a given directory.

From c2a374fba137fee0829876e0e275ddce76e01e83 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 15:55:46 +0000
Subject: [PATCH 61/85] The convention is now to put all the pipeline_info
 files in per-pipeline sub-directories

---
 docs/output.md            | 2 +-
 lib/NfcoreTemplate.groovy | 2 +-
 nextflow.config           | 2 +-
 nextflow_schema.json      | 2 +-
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 30b1452..009197f 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -75,7 +75,7 @@ the directory structure includes the assembly name, e.g. `gfLaeSulp1.1`, and all
 
 ### Pipeline information
 
-- `pipeline_info/`
+- `pipeline_info/insdcdownload/`
   - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
   - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
   - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy
index c534916..85785ef 100755
--- a/lib/NfcoreTemplate.groovy
+++ b/lib/NfcoreTemplate.groovy
@@ -132,7 +132,7 @@ class NfcoreTemplate {
         }
 
         // Write summary e-mail HTML to a file
-        def output_d = new File("${params.outdir}/pipeline_info/")
+        def output_d = new File("${params.tracedir}/")
         if (!output_d.exists()) {
             output_d.mkdirs()
         }
diff --git a/nextflow.config b/nextflow.config
index f3d56bb..f1e4a27 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -17,7 +17,7 @@ params {
 
     // Boilerplate options
     outdir                     = 'results'
-    tracedir                   = "${params.outdir}/pipeline_info"
+    tracedir                   = "${params.outdir}/pipeline_info/insdcdownload"
     publish_dir_mode           = 'copy'
     email                      = null
     email_on_fail              = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 4af1a8a..053a320 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -198,7 +198,7 @@
                 "tracedir": {
                     "type": "string",
                     "description": "Directory to keep pipeline Nextflow logs and reports.",
-                    "default": "${params.outdir}/pipeline_info",
+                    "default": "${params.outdir}/pipeline_info/insdcdownload",
                     "fa_icon": "fas fa-cogs",
                     "hidden": true
                 },

From 7777314032c56f4bb9d62455bf54bd64ebe28a41 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 16:42:07 +0000
Subject: [PATCH 62/85] Added the code of conduct back

---
 .nf-core.yml       |   1 -
 CODE_OF_CONDUCT.md | 111 +++++++++++++++++++++++++++++++++++++++++++++
 2 files changed, 111 insertions(+), 1 deletion(-)
 create mode 100644 CODE_OF_CONDUCT.md

diff --git a/.nf-core.yml b/.nf-core.yml
index ad4c83b..d524328 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,7 +1,6 @@
 repository_type: pipeline
 lint:
   files_exist:
-    - CODE_OF_CONDUCT.md
     - assets/multiqc_config.yml
     - assets/nf-core-insdcdownload_logo_light.png
     - conf/igenomes.config
diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md
new file mode 100644
index 0000000..f4fd052
--- /dev/null
+++ b/CODE_OF_CONDUCT.md
@@ -0,0 +1,111 @@
+# Code of Conduct at nf-core (v1.0)
+
+## Our Pledge
+
+In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of:
+
+- Age
+- Body size
+- Familial status
+- Gender identity and expression
+- Geographical location
+- Level of experience
+- Nationality and national origins
+- Native language
+- Physical and neurological ability
+- Race or ethnicity
+- Religion
+- Sexual identity and orientation
+- Socioeconomic status
+
+Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance.
+
+## Preamble
+
+> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply.
+
+An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva.
+
+nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals.
+
+We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc.
+
+Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities.
+
+We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC.
+
+Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re
+
+## Our Responsibilities
+
+The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour.
+
+The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful.
+
+Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC.
+
+## When are where does this Code of Conduct apply?
+
+Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference:
+
+- Communicating with an official project email address.
+- Communicating with community members within the nf-core Slack channel.
+- Participating in hackathons organised by nf-core (both online and in-person events).
+- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence.
+- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc.
+- Representing nf-core on social media. This includes both official and personal accounts.
+
+## nf-core cares 😊
+
+nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order):
+
+- Ask for consent before sharing another community member’s personal information (including photographs) on social media.
+- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity.
+- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !)
+- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.)
+- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can)
+- Focus on what is best for the team and the community. (When in doubt, ask)
+- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn.
+- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!)
+- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**)
+- Take breaks when you feel like you need them.
+- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.)
+
+## nf-core frowns on 😕
+
+The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces.
+
+- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom.
+- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online.
+- Spamming or trolling of individuals on social media.
+- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention.
+- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience.
+
+### Online Trolling
+
+The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately.
+
+All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls.
+
+## Procedures for Reporting CoC violations
+
+If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible.
+
+You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s).
+
+Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course.
+
+All reports will be handled with utmost discretion and confidentially.
+
+## Attribution and Acknowledgements
+
+- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4)
+- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition)
+- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/)
+- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla)
+
+## Changelog
+
+### v1.0 - March 12th, 2021
+
+- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC.

From 00badc411cbeb9f4c7c1371d9367df909706ef8f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 16:42:36 +0000
Subject: [PATCH 63/85] There is no parameters.md page anymore

---
 .nf-core.yml   | 1 -
 docs/README.md | 2 --
 2 files changed, 3 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index d524328..5a3fa08 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -15,7 +15,6 @@ lint:
     - .github/ISSUE_TEMPLATE/bug_report.yml
     - .github/workflows/linting.yml
     - assets/sendmail_template.txt
-    - docs/README.md
     - lib/NfcoreTemplate.groovy
   nextflow_config:
     - manifest.name
diff --git a/docs/README.md b/docs/README.md
index 7b74c4f..60d64f6 100644
--- a/docs/README.md
+++ b/docs/README.md
@@ -4,7 +4,5 @@ The sanger-tol/insdcdownload documentation is split into the following pages:
 
 - [Usage](usage.md)
   - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags.
-- [Parameters](parameters.md)
-  - An overview of the different pipeline options and how to use them.
 - [Output](output.md)
   - An overview of the different results produced by the pipeline and how to interpret them.

From 3c42dfa4a30328926f60d9c8ca6335053868db40 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 31 Jul 2023 17:14:25 +0000
Subject: [PATCH 64/85] Fixed README - now matching the template and linking to
 the pipeline website

---
 README.md | 15 ++++-----------
 1 file changed, 4 insertions(+), 11 deletions(-)

diff --git a/README.md b/README.md
index 21eae34..010c005 100644
--- a/README.md
+++ b/README.md
@@ -19,14 +19,6 @@
 
 **sanger-tol/insdcdownload** is a pipeline that downloads assemblies from INSDC into a Tree of Life directory structure.
 
-The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
-
-On release, automated continuous integration tests run the pipeline on a full-sized dataset on the GitHub CI infrastructure. This ensures that the pipeline runs in a third-party environment, and has sensible resource allocation defaults set to run on real-world datasets.
-
-## Pipeline summary
-
-## Overview
-
 The pipeline takes an assembly accession number, as well as the assembly name, and downloads it. It also builds a set of common indices (such as `samtools faidx`), and extracts the repeat-masking performed by the NCBI.
 
 Steps involved:
@@ -48,6 +40,8 @@ Steps involved:
 > to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline)
 > with `-profile test` before running the workflow on actual data.
 
+The easiest is to provide the exact name and accession number of the assembly like this:
+
 ```console
 nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir results
 ```
@@ -57,9 +51,8 @@ nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --ass
 > provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
 > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
 
-## Documentation
-
-The sanger-tol/insdcdownload pipeline comes with documentation about the pipeline [usage](docs/usage.md) and [output](docs/output.md).
+The pipeline also supports bulk downloads through a sample-sheet.
+More information about this mode on our [pipeline website](https://pipelines.tol.sanger.ac.uk/insdcdownload/usage).
 
 ## Credits
 

From 44fbe8b3c86676284b4729c37b8b648be7e2e8dc Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 3 Aug 2023 17:34:04 +0000
Subject: [PATCH 65/85] Updated the DOI

---
 CITATION.cff            | 2 +-
 README.md               | 4 ++--
 lib/WorkflowMain.groovy | 2 +-
 nextflow.config         | 2 +-
 4 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/CITATION.cff b/CITATION.cff
index b7b4573..3adf05f 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -18,7 +18,7 @@ authors:
       affiliation: Wellcome Sanger Institute
 identifiers:
     - type: doi
-      value: 10.5281/zenodo.7155119
+      value: 10.5281/zenodo.6983932
 repository-code: "https://github.com/sanger-tol/insdcdownload"
 license: MIT
 commit: TODO
diff --git a/README.md b/README.md
index 010c005..ecae670 100644
--- a/README.md
+++ b/README.md
@@ -4,7 +4,7 @@
 
 <!-- [![GitHub Actions Linting Status](https://github.com/sanger-tol/insdcdownload/workflows/nf-core%20linting/badge.svg)](https://github.com/sanger-tol/insdcdownload/actions?query=workflow%3A%22nf-core+linting%22) -->
 
-[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.7155119-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.7155119)
+[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.6983932-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.6983932)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
@@ -66,7 +66,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 
 ## Citations
 
-If you use sanger-tol/insdcdownload for your analysis, please cite it using the following doi: [10.5281/zenodo.7155119](https://doi.org/10.5281/zenodo.7155119)
+If you use sanger-tol/insdcdownload for your analysis, please cite it using the following doi: [10.5281/zenodo.6983932](https://doi.org/10.5281/zenodo.6983932)
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy
index 815e847..a572108 100755
--- a/lib/WorkflowMain.groovy
+++ b/lib/WorkflowMain.groovy
@@ -12,7 +12,7 @@ class WorkflowMain {
     public static String citation(workflow) {
         return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
             "* The pipeline\n" +
-            "  https://doi.org/10.5281/zenodo.7155119\n\n" +
+            "  https://doi.org/10.5281/zenodo.6983932\n\n" +
             "* The nf-core framework\n" +
             "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
             "* Software dependencies\n" +
diff --git a/nextflow.config b/nextflow.config
index f1e4a27..d977225 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -205,7 +205,7 @@ manifest {
     mainScript      = 'main.nf'
     nextflowVersion = '!>=22.10.1'
     version         = '1.2dev'
-    doi             = ''
+    doi             = '10.5281/zenodo.6983932'
 }
 
 // Load modules.config for DSL2 module specific options

From 24eea75ff5008f7353b42dc2cf21d0c486da9746 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 3 Aug 2023 17:34:16 +0000
Subject: [PATCH 66/85] This is superfluous

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index ecae670..33553f1 100644
--- a/README.md
+++ b/README.md
@@ -43,7 +43,7 @@ Steps involved:
 The easiest is to provide the exact name and accession number of the assembly like this:
 
 ```console
-nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir results
+nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1
 ```
 
 > **Warning:**

From a94039d4ac85460f09a08a7ee700b1def70c4402 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 3 Aug 2023 17:34:28 +0000
Subject: [PATCH 67/85] Updated the contributions

---
 README.md | 6 +++++-
 1 file changed, 5 insertions(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 33553f1..2e62502 100644
--- a/README.md
+++ b/README.md
@@ -56,7 +56,11 @@ More information about this mode on our [pipeline website](https://pipelines.tol
 
 ## Credits
 
-sanger-tol/insdcdownload was mainly written by @muffato, with major borrowings from @priyanka-surana's [read-mapping](https://github.com/sanger-tol/readmapping) pipeline, e.g. the script to remove the repeat-masking, and the overall structure and layout of the sub-workflows.
+sanger-tol/insdcdownload was mainly written by [Matthieu Muffato](https://github.com/muffato), with major borrowings from a's [read-mapping](https://github.com/sanger-tol/readmapping) pipeline, e.g. the script to remove the repeat-masking, and the overall structure and layout of the sub-workflows.
+
+We thank the following people for their assistance in the development of this pipeline:
+
+- [Priyanka Surana](https://github.com/priyanka-surana) for providing reviews.
 
 ## Contributions and Support
 

From 322ca6cb980cba724a414fc4046f07ec64ca184c Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 3 Aug 2023 18:09:42 +0000
Subject: [PATCH 68/85] Added options to submit the pipelines to the farm from
 GitHub

---
 .github/workflows/sangerfulltest.yml | 4 ++--
 .github/workflows/sangertest.yml     | 4 ++--
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sangerfulltest.yml
index 9b68e67..850d4ae 100644
--- a/.github/workflows/sangerfulltest.yml
+++ b/.github/workflows/sangerfulltest.yml
@@ -1,4 +1,4 @@
-name: nf-core Sanger LSF full size tests
+name: sanger-tol LSF full size tests
 
 on:
   push:
@@ -34,4 +34,4 @@ jobs:
             {
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ env.REVISION }}",
             }
-          profiles: test_full,sanger,singularity
+          profiles: test_full,sanger,singularity,cleanup
diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sangertest.yml
index f9befb3..d6d7be5 100644
--- a/.github/workflows/sangertest.yml
+++ b/.github/workflows/sangertest.yml
@@ -1,4 +1,4 @@
-name: nf-core Sanger LSF tests
+name: sanger-tol LSF tests
 
 on:
   workflow_dispatch:
@@ -20,4 +20,4 @@ jobs:
             {
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ github.sha }}",
             }
-          profiles: test,sanger,singularity
+          profiles: test,sanger,singularity,cleanup

From e1b927d126a365318162ea0dfba5c2f5322f68dc Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 4 Aug 2023 11:28:43 +0100
Subject: [PATCH 69/85] docfix

---
 nextflow_schema.json | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 053a320..90a125c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -27,7 +27,8 @@
                 "outdir": {
                     "type": "string",
                     "format": "directory-path",
-                    "description": "The output directory where the results will be saved. Not considered when running the pipeline with a .csv file as input.",
+                    "description": "The output directory where the results will be saved. Not considered for sample-sheet entries that have an absolute path.",
+                    "default": "results",
                     "fa_icon": "fas fa-folder-open"
                 },
                 "input": {

From e24a6f7154878410891556e06125ec9f3f3736b4 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 10 Aug 2023 16:50:15 +0000
Subject: [PATCH 70/85] Latest nomenclature

---
 .github/workflows/{sangertest.yml => sanger_test.yml}          | 0
 .github/workflows/{sangerfulltest.yml => sanger_test_full.yml} | 0
 2 files changed, 0 insertions(+), 0 deletions(-)
 rename .github/workflows/{sangertest.yml => sanger_test.yml} (100%)
 rename .github/workflows/{sangerfulltest.yml => sanger_test_full.yml} (100%)

diff --git a/.github/workflows/sangertest.yml b/.github/workflows/sanger_test.yml
similarity index 100%
rename from .github/workflows/sangertest.yml
rename to .github/workflows/sanger_test.yml
diff --git a/.github/workflows/sangerfulltest.yml b/.github/workflows/sanger_test_full.yml
similarity index 100%
rename from .github/workflows/sangerfulltest.yml
rename to .github/workflows/sanger_test_full.yml

From 1b7f285d6e9cb07ef6645241f6931f5c2f3931d8 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Thu, 3 Aug 2023 16:29:32 +0000
Subject: [PATCH 71/85] Now use "outdir", which is equivalent to a ToL analysis
 directory

---
 CHANGELOG.md                          |  4 ++-
 assets/samplesheet.csv                | 14 ++++----
 assets/schema_input.json              |  4 +--
 bin/check_samplesheet.py              | 12 +++----
 conf/modules.config                   |  6 ++--
 docs/output.md                        | 50 ++++++++++++---------------
 docs/usage.md                         |  8 ++---
 modules/local/ncbi_download.nf        |  6 ++--
 nextflow_schema.json                  |  2 +-
 subworkflows/local/download_genome.nf |  2 +-
 subworkflows/local/params_check.nf    |  6 ++--
 11 files changed, 54 insertions(+), 60 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index b518284..e405031 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,10 +3,12 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v1.2.0 - [date]
+## v2.0.0 - [date]
 
 ### `Fixed`
 
+- The sample-sheet column `species_dir` is replaced with the `outdir` column which
+  represents where the assembly and repeats are downloaded (in immediate sub-directories)
 - Relative paths in the sample-sheet are now evaluated from the `--outdir` parameter
 - Memory usage rules for `samtools dict`
 - Appropriate use of `tabix`'s TBI and CSI indexing, depending on the sequence lengths
diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
index 49f1be2..0f2f8be 100644
--- a/assets/samplesheet.csv
+++ b/assets/samplesheet.csv
@@ -1,7 +1,7 @@
-species_dir,assembly_name,assembly_accession
-25g/data/echinoderms/Asterias_rubens,eAstRub1.3,GCA_902459465.3
-25g/data/insects/Osmia_bicornis,iOsmBic2.1,GCA_907164935.1
-25g/data/insects/Osmia_bicornis,iOsmBic2.1_alternate_haplotype,GCA_907164925.1
-darwin/data/fungi/Laetiporus_sulphureus,gfLaeSulp1.1,GCA_927399515.1
-darwin/data/insects/Noctua_fimbriata,ilNocFimb1.1,GCA_905163415.1
-darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
+outdir,assembly_name,assembly_accession
+Asterias_rubens/eAstRub1.3,eAstRub1.3,GCA_902459465.3
+Osmia_bicornis/iOsmBic2.1,iOsmBic2.1,GCA_907164935.1
+Osmia_bicornis/iOsmBic2.1_alternate_haplotype,iOsmBic2.1_alternate_haplotype,GCA_907164925.1
+Laetiporus_sulphureus/gfLaeSulp1.1,gfLaeSulp1.1,GCA_927399515.1
+Noctua_fimbriata/ilNocFimb1.1,ilNocFimb1.1,GCA_905163415.1
+Meles_meles/mMelMel3.2_paternal_haplotype,mMelMel3.2_paternal_haplotype,GCA_922984935.2
diff --git a/assets/schema_input.json b/assets/schema_input.json
index ed91197..dafbfaa 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -7,7 +7,7 @@
     "items": {
         "type": "object",
         "properties": {
-            "species_dir": {
+            "outdir": {
                 "type": "string",
                 "pattern": "^\\S+$",
                 "errorMessage": "Species directory must be provided and exist"
@@ -23,6 +23,6 @@
                 "errorMessage": "Assembly accession number must be provided and be of the form GCA_*"
             }
         },
-        "required": ["species_dir", "assembly_name", "assembly_accession"]
+        "required": ["outdir", "assembly_name", "assembly_accession"]
     }
 }
diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index de6852e..aa8bfc4 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -28,7 +28,7 @@ class RowChecker:
 
     def __init__(
         self,
-        dir_col="species_dir",
+        dir_col="outdir",
         name_col="assembly_name",
         accession_col="assembly_accession",
         **kwargs,
@@ -38,7 +38,7 @@ def __init__(
 
         Args:
             dir_col (str): The name of the column that contains the species directory
-                (default "species_dir").
+                (default "outdir").
             name_col (str): The name of the column that contains the assembly name
                 (default "assembly_name").
             accession_col (str): The name of the column that contains the accession
@@ -142,12 +142,12 @@ def check_samplesheet(file_in, file_out):
     Example:
         This function checks that the samplesheet follows the following structure::
 
-            species_dir,assembly_name,assembly_accession
-            darwin/data/fungi/Laetiporus_sulphureus,gfLaeSulp1.1,GCA_927399515.1
-            darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
+            outdir,assembly_name,assembly_accession
+            Laetiporus_sulphureus/gfLaeSulp1.1,gfLaeSulp1.1,GCA_927399515.1
+            Meles_meles/mMelMel3.2_paternal_haplotype,mMelMel3.2_paternal_haplotype,GCA_922984935.2
     """
     required_columns = {
-        "species_dir",
+        "outdir",
         "assembly_name",
         "assembly_accession",
     }
diff --git a/conf/modules.config b/conf/modules.config
index 091bce5..c0bcbe4 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -15,7 +15,7 @@ process {
     withName: 'NCBI_DOWNLOAD' {
         maxForks = 3
         publishDir = [
-            path: { "${meta.species_dir}/assembly/release/${meta.assembly_name}/insdc" },
+            path: { "${meta.outdir}/assembly" },
             mode: 'copy',
             saveAs: { filename -> filename.endsWith('assembly_report.txt') || filename.endsWith('assembly_stats.txt') || filename.endsWith("ACCESSION") ? filename : null }
         ]
@@ -23,7 +23,7 @@ process {
 
     withName: '.*:.*:PREPARE_UNMASKED_FASTA:.*' {
         publishDir = [
-            path: { "${meta.species_dir}/assembly/release/${meta.assembly_name}/insdc" },
+            path: { "${meta.outdir}/assembly" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -31,7 +31,7 @@ process {
 
     withName: '.*:.*:(PREPARE_REPEAT_MASKED_FASTA:.*|PREPARE_REPEATS:TABIX_.*)' {
         publishDir = [
-            path: { "${meta.species_dir}/analysis/${meta.assembly_name}/repeats/ncbi" },
+            path: { "${meta.outdir}/repeats/ncbi" },
             mode: 'copy',
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/docs/output.md b/docs/output.md
index 009197f..312f315 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -4,7 +4,8 @@
 
 This document describes the output produced by the pipeline.
 
-The directories listed below will be created in the results directory (or `species_dir` when using a samplesheet) after the pipeline has finished.
+The directories listed below will be created in a directory based on the `--outdir` command-line parameter and the `outdir` column of the samplesheet.
+) after the pipeline has finished.
 All paths are relative to the top-level results directory.
 
 The directories comply with Tree of Life's canonical directory structure.
@@ -23,20 +24,17 @@ Here are the files you can expect in the `assembly/` sub-directory.
 
 ```text
 assembly
-└── release
-    └── gfLaeSulp1.1
-        └── insdc
-            ├── ACCESSION
-            ├── GCA_927399515.1.assembly_report.txt
-            ├── GCA_927399515.1.assembly_stats.txt
-            ├── GCA_927399515.1.fa.dict
-            ├── GCA_927399515.1.fa.gz
-            ├── GCA_927399515.1.fa.gz.fai
-            ├── GCA_927399515.1.fa.gz.gzi
-            └── GCA_927399515.1.fa.gz.sizes
+├── ACCESSION
+├── GCA_927399515.1.assembly_report.txt
+├── GCA_927399515.1.assembly_stats.txt
+├── GCA_927399515.1.fa.dict
+├── GCA_927399515.1.fa.gz
+├── GCA_927399515.1.fa.gz.fai
+├── GCA_927399515.1.fa.gz.gzi
+└── GCA_927399515.1.fa.gz.sizes
 ```
 
-The directory structure includes the assembly name, e.g. `gfLaeSulp1.1`, and all files are named after the assembly accession, e.g. `GCA_927399515.1`.
+All files are named after the assembly accession, e.g. `GCA_927399515.1`.
 
 - `GCA_*.assembly_report.txt` and `GCA_*.assembly_stats.txt`: report and statistics files, straight from the NCBI FTP
 - `GCA_*.fa.gz`: Unmasked assembly in Fasta format, compressed with `bgzip` (whose index is `GCA_*.fa.gz.gzi`)
@@ -48,25 +46,23 @@ with the exception of `ACCESSION`, which contains a single line of text: the ass
 
 ### Primary analysis files
 
-Here are the files you can expect in the `analysis/` sub-directory.
+Here are the files you can expect in the `repeats/` sub-directory.
 
 ```text
-analysis
-└── gfLaeSulp1.1
-    └── repeats
-        └── ncbi
-            ├── GCA_927399515.1.masked.ncbi.bed.gz
-            ├── GCA_927399515.1.masked.ncbi.bed.gz.csi
-            ├── GCA_927399515.1.masked.ncbi.bed.gz.tbi
-            ├── GCA_927399515.1.masked.ncbi.fa.dict
-            ├── GCA_927399515.1.masked.ncbi.fa.gz
-            ├── GCA_927399515.1.masked.ncbi.fa.gz.fai
-            ├── GCA_927399515.1.masked.ncbi.fa.gz.gzi
-            └── GCA_927399515.1.masked.ncbi.fa.gz.sizes
+repeats
+└── ncbi
+    ├── GCA_927399515.1.masked.ncbi.bed.gz
+    ├── GCA_927399515.1.masked.ncbi.bed.gz.csi
+    ├── GCA_927399515.1.masked.ncbi.bed.gz.tbi
+    ├── GCA_927399515.1.masked.ncbi.fa.dict
+    ├── GCA_927399515.1.masked.ncbi.fa.gz
+    ├── GCA_927399515.1.masked.ncbi.fa.gz.fai
+    ├── GCA_927399515.1.masked.ncbi.fa.gz.gzi
+    └── GCA_927399515.1.masked.ncbi.fa.gz.sizes
 ```
 
 They all correspond to the repeat-masking analysis run by the NCBI themselves. Like for the `assembly/` sub-directory,
-the directory structure includes the assembly name, e.g. `gfLaeSulp1.1`, and all files are named after the assembly accession, e.g. `GCA_927399515.1`.
+all files are named after the assembly accession, e.g. `GCA_927399515.1`.
 
 - `GCA_*.masked.ncbi.fa.gz`: Masked assembly in Fasta format, compressed with `bgzip` (whose index is `GCA_*.fa.gz.gzi`)
 - `GCA_*.masked.ncbi.fa.gz.fai`: `samtools faidx` index, which allows accessing any region of the assembly in constant time
diff --git a/docs/usage.md b/docs/usage.md
index 0b120bc..d6c683f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -30,14 +30,14 @@ The pipeline can download multiple assemblies at once, by providing them in a `.
 It has to be a comma-separated file with three columns, and a header row as shown in the examples below.
 
 ```console
-species_dir,assembly_name,assembly_accession
+outdir,assembly_name,assembly_accession
 darwin/data/fungi/Laetiporus_sulphureus,gfLaeSulp1.1,GCA_927399515.1
 darwin/data/mammals/Meles_meles,mMelMel3.2_paternal_haplotype,GCA_922984935.2
 ```
 
 | Column               | Description                                                                      |
 | -------------------- | -------------------------------------------------------------------------------- |
-| `species_dir`        | Base download directory for this species. Evaluated from `--outdir` if relative. |
+| `outdir`             | Base download directory for this species. Evaluated from `--outdir` if relative. |
 | `assembly_name`      | Name of the assembly, as on the NCBI website, e.g. `gfLaeSulp1.1`.               |
 | `assembly_accession` | Accession number of the assembly to download. Typically of the form `GCA_*.*`.   |
 
@@ -48,9 +48,7 @@ A samplesheet may contain:
 - only one row per assembly
 
 All samplesheet columns correspond exactly to their corresponding command-line parameter,
-except `species_dir` which overrides or complements `--oudir`.
-`species_dir` is used to fit the output of this pipeline into a directory structure compatible with the other pipelines
-from Sanger Tree of Life.
+except `outdir` which overrides or complements `--oudir`.
 
 An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline.
 
diff --git a/modules/local/ncbi_download.nf b/modules/local/ncbi_download.nf
index 54196d2..ae63e52 100644
--- a/modules/local/ncbi_download.nf
+++ b/modules/local/ncbi_download.nf
@@ -12,7 +12,7 @@ process NCBI_DOWNLOAD {
         'biocontainers/gnu-wget:1.18--h7132678_6' }"
 
     input:
-    tuple val(assembly_accession), val(assembly_name), val(species_dir)
+    tuple val(assembly_accession), val(assembly_name), val(outdir)
 
     output:
     tuple val(meta), path(filename_fasta)          , emit: fasta
@@ -36,7 +36,7 @@ process NCBI_DOWNLOAD {
     meta = [
         id : assembly_accession,
         assembly_name : assembly_name,
-        species_dir : species_dir,
+        outdir : outdir,
     ]
     def prefix = task.ext.prefix ?: "${meta.id}"
     filename_assembly_report = "${prefix}.assembly_report.txt"
@@ -45,8 +45,6 @@ process NCBI_DOWNLOAD {
     filename_accession = "ACCESSION"
 
     """
-    #export https_proxy=http://wwwcache.sanger.ac.uk:3128
-    #export http_proxy=http://wwwcache.sanger.ac.uk:3128
     wget ${ftp_path}/${remote_filename_stem}_assembly_report.txt
     wget ${ftp_path}/${remote_filename_stem}_assembly_stats.txt
     wget ${ftp_path}/${remote_filename_stem}_genomic.fna.gz
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 90a125c..bc19c9b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -38,7 +38,7 @@
                     "pattern": "^\\S+\\.csv$",
                     "schema": "assets/schema_input.json",
                     "description": "Path to comma-separated file containing information about the assemblies to download. Used for bulk download of many assemblies.",
-                    "help_text": "The file has to be a comma-separated file with three columns, and a header row. The columns names must be `species_dir`, `assembly_accession`, and `assembly_name`.",
+                    "help_text": "The file has to be a comma-separated file with three columns, and a header row. The columns names must be `outdir`, `assembly_accession`, and `assembly_name`.",
                     "fa_icon": "fas fa-file-csv"
                 },
                 "ftp_root": {
diff --git a/subworkflows/local/download_genome.nf b/subworkflows/local/download_genome.nf
index 0a57503..dd4da55 100644
--- a/subworkflows/local/download_genome.nf
+++ b/subworkflows/local/download_genome.nf
@@ -9,7 +9,7 @@ include { REMOVE_MASKING          } from '../../modules/local/remove_masking'
 workflow DOWNLOAD_GENOME {
 
     take:
-    assembly_params         // tuple(assembly_accession, assembly_name, species_dir)
+    assembly_params         // tuple(assembly_accession, assembly_name, outdir)
 
 
     main:
diff --git a/subworkflows/local/params_check.nf b/subworkflows/local/params_check.nf
index 8992365..e9e4458 100644
--- a/subworkflows/local/params_check.nf
+++ b/subworkflows/local/params_check.nf
@@ -21,13 +21,13 @@ workflow PARAMS_CHECK {
 
         SAMPLESHEET_CHECK ( file(samplesheet, checkIfExists: true) )
             .csv
-            // Provides species_dir, assembly_accession, and assembly_name
+            // Provides outdir, assembly_accession, and assembly_name
             .splitCsv ( header:true, sep:',' )
             // Convert to tuple, as required by the download subworkflow
             .map { [
                 it["assembly_accession"],
                 it["assembly_name"],
-                (it["species_dir"].startsWith("/") ? "" : outdir + "/") + it["species_dir"],
+                (it["outdir"].startsWith("/") ? "" : outdir + "/") + it["outdir"],
             ] }
             .set { ch_inputs }
 
@@ -41,7 +41,7 @@ workflow PARAMS_CHECK {
 
 
     emit:
-    assembly_params = ch_inputs        // channel: tuple(assembly_accession, assembly_name, species_dir)
+    assembly_params = ch_inputs        // channel: tuple(assembly_accession, assembly_name, outdir)
     versions        = ch_versions      // channel: versions.yml
 }
 

From 9f1663137fce925a0ea17f0e0b997e89a610ac8f Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Mon, 14 Aug 2023 13:24:18 +0000
Subject: [PATCH 72/85] Use the latest version of the Tower action

---
 .github/workflows/sanger_test.yml      | 2 +-
 .github/workflows/sanger_test_full.yml | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/.github/workflows/sanger_test.yml b/.github/workflows/sanger_test.yml
index d6d7be5..e7007c0 100644
--- a/.github/workflows/sanger_test.yml
+++ b/.github/workflows/sanger_test.yml
@@ -8,7 +8,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v2
+        uses: seqeralabs/action-tower-launch@v2
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
diff --git a/.github/workflows/sanger_test_full.yml b/.github/workflows/sanger_test_full.yml
index 850d4ae..fc11b07 100644
--- a/.github/workflows/sanger_test_full.yml
+++ b/.github/workflows/sanger_test_full.yml
@@ -22,7 +22,7 @@ jobs:
         if: github.event_name == 'workflow_dispatch'
 
       - name: Launch workflow via tower
-        uses: nf-core/tower-action@v2
+        uses: seqeralabs/action-tower-launch@v2
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}

From b4eb799a12a7dae570d9046a5a28e27761a7d81b Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 16 Aug 2023 14:03:59 +0100
Subject: [PATCH 73/85] Update sanger_test_full.yml, remove variable pipeline
 and upload artifacts

---
 .github/workflows/sanger_test_full.yml | 8 +++++++-
 1 file changed, 7 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/sanger_test_full.yml b/.github/workflows/sanger_test_full.yml
index fc11b07..01f0cb1 100644
--- a/.github/workflows/sanger_test_full.yml
+++ b/.github/workflows/sanger_test_full.yml
@@ -27,7 +27,6 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          pipeline: ${{ github.repository }}
           revision: ${{ env.REVISION }}
           workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/${{ github.repository }}/work-${{ env.REVISION }}
           parameters: |
@@ -35,3 +34,10 @@ jobs:
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ env.REVISION }}",
             }
           profiles: test_full,sanger,singularity,cleanup
+
+      - uses: actions/upload-artifact@v3
+        with:
+          name: Tower debug log file
+          path: |
+            tower_action_*.log
+            tower_action_*.json      

From 4fee623178ae2adfed3ae96fd9de8a8c8b97846b Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 16 Aug 2023 14:05:05 +0100
Subject: [PATCH 74/85] Update sanger_test.yml - remove pipeline variable and
 upload artifacts

---
 .github/workflows/sanger_test.yml | 8 +++++++-
 1 file changed, 7 insertions(+), 1 deletion(-)

diff --git a/.github/workflows/sanger_test.yml b/.github/workflows/sanger_test.yml
index e7007c0..6887663 100644
--- a/.github/workflows/sanger_test.yml
+++ b/.github/workflows/sanger_test.yml
@@ -13,7 +13,6 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          pipeline: ${{ github.repository }}
           revision: ${{ github.sha }}
           workdir: ${{ secrets.TOWER_WORKDIR_PARENT }}/work/${{ github.repository }}/work-${{ github.sha }}
           parameters: |
@@ -21,3 +20,10 @@ jobs:
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ github.sha }}",
             }
           profiles: test,sanger,singularity,cleanup
+ 
+      - uses: actions/upload-artifact@v3
+        with:
+          name: Tower debug log file
+          path: |
+            tower_action_*.log
+            tower_action_*.json          

From 706a599bfd9f6425e7edb1879be6d2dba27fd618 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 16 Aug 2023 14:50:35 +0100
Subject: [PATCH 75/85] Update sanger_test.yml

---
 .github/workflows/sanger_test.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/sanger_test.yml b/.github/workflows/sanger_test.yml
index 6887663..b482e93 100644
--- a/.github/workflows/sanger_test.yml
+++ b/.github/workflows/sanger_test.yml
@@ -26,4 +26,4 @@ jobs:
           name: Tower debug log file
           path: |
             tower_action_*.log
-            tower_action_*.json          
+            tower_action_*.json

From 231a0977f2457118f84910ab3889eec92798582f Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 16 Aug 2023 14:50:55 +0100
Subject: [PATCH 76/85] Update sanger_test_full.yml

---
 .github/workflows/sanger_test_full.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/sanger_test_full.yml b/.github/workflows/sanger_test_full.yml
index 01f0cb1..e028c6b 100644
--- a/.github/workflows/sanger_test_full.yml
+++ b/.github/workflows/sanger_test_full.yml
@@ -40,4 +40,4 @@ jobs:
           name: Tower debug log file
           path: |
             tower_action_*.log
-            tower_action_*.json      
+            tower_action_*.json

From b2c6f82a15b888d34466aef3b841fba09e9c07ef Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Wed, 16 Aug 2023 14:56:01 +0100
Subject: [PATCH 77/85] Update sanger_test.yml

---
 .github/workflows/sanger_test.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/sanger_test.yml b/.github/workflows/sanger_test.yml
index b482e93..e69af1e 100644
--- a/.github/workflows/sanger_test.yml
+++ b/.github/workflows/sanger_test.yml
@@ -20,7 +20,7 @@ jobs:
               "outdir": "${{ secrets.TOWER_WORKDIR_PARENT }}/results/${{ github.repository }}/results-${{ github.sha }}",
             }
           profiles: test,sanger,singularity,cleanup
- 
+
       - uses: actions/upload-artifact@v3
         with:
           name: Tower debug log file

From 9c20644cc17d4b2fa81bb215b028efa06a757c22 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 18 Aug 2023 15:17:54 +0000
Subject: [PATCH 78/85] New tolsoft details

---
 .github/workflows/fix-linting.yml | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index d8478c7..010b9f1 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -8,21 +8,21 @@ jobs:
     # Only run if comment is on a PR with the main repo, and if it contains the magic keywords
     if: >
       contains(github.event.comment.html_url, '/pull/') &&
-      contains(github.event.comment.body, '@nf-core-bot fix linting') &&
-      github.repository == 'sanger-tol/insdcdownload'
+      contains(github.event.comment.body, '@sanger-tolsoft fix linting') &&
+      github.repository == 'sanger-tol/variantcalling'
     runs-on: ubuntu-latest
     steps:
-      # Use the @nf-core-bot token to check out so we can push later
+      # Use the @sanger-tolsoft token to check out so we can push later
       - uses: actions/checkout@v3
         with:
-          token: ${{ secrets.nf_core_bot_auth_token }}
+          token: ${{ secrets.sangertolsoft_access_token }}
 
       # Action runs on the issue comment, so we don't get the PR by default
       # Use the gh cli to check out the PR
       - name: Checkout Pull Request
         run: gh pr checkout ${{ github.event.issue.number }}
         env:
-          GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
+          GITHUB_TOKEN: ${{ secrets.sangertolsoft_access_token }}
 
       - uses: actions/setup-node@v3
 
@@ -46,8 +46,8 @@ jobs:
       - name: Commit & push changes
         if: steps.prettier_status.outputs.result == 'fail'
         run: |
-          git config user.email "core@nf-co.re"
-          git config user.name "nf-core-bot"
+          git config user.email "105875386+sanger-tolsoft@users.noreply.github.com"
+          git config user.name "sanger-tolsoft"
           git config push.default upstream
           git add .
           git status

From c2dba627afece076f3885ea717f13333d020c7bd Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 18 Aug 2023 15:29:16 +0000
Subject: [PATCH 79/85] Fixed the repository name

---
 .github/workflows/fix-linting.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 010b9f1..324f123 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -9,7 +9,7 @@ jobs:
     if: >
       contains(github.event.comment.html_url, '/pull/') &&
       contains(github.event.comment.body, '@sanger-tolsoft fix linting') &&
-      github.repository == 'sanger-tol/variantcalling'
+      github.repository == 'sanger-tol/insdcdownload'
     runs-on: ubuntu-latest
     steps:
       # Use the @sanger-tolsoft token to check out so we can push later

From 403f469d084d0631e0135f2cace320fdd4219491 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Sat, 23 Sep 2023 11:18:38 +0000
Subject: [PATCH 80/85] Made the relation between --outdir and the samplesheet
 outdir clearer

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index d6c683f..f785a05 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -15,7 +15,7 @@ The pipeline accepts command-one line arguments to specify a single genome to do
 
 - `--assembly_name`: The name of the assembly,
 - `--assembly_accession`: The accession number of the assembly,
-- `--outdir`: Where to download the data.
+- `--outdir`: Where the pipeline runtime information will be stored, and where data will be downloaded (except if absolute paths are given in the samplesheet).
 
 ```console
 nextflow run sanger-tol/insdcdownload --assembly_accession GCA_927399515.1 --assembly_name gfLaeSulp1.1 --outdir gfLaeSulp1.1_data

From ffe15b9077959f712b48a0e7bbc7f968d8c2e4a2 Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 24 May 2024 11:26:37 +0000
Subject: [PATCH 81/85] Version bump

---
 CHANGELOG.md    | 29 +++++++++++++++++++++++------
 CITATION.cff    |  4 ++--
 nextflow.config |  2 +-
 3 files changed, 26 insertions(+), 9 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e405031..6d8a09e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,21 +3,38 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v2.0.0 - [date]
+## [[2.0.0](https://github.com/sanger-tol/insdcdownload/releases/tag/2.0.0)] – Light elf – [2024-05-24]
 
-### `Fixed`
+This version supports the new FTP structure of Ensembl
+
+### Enhancements & fixes
 
 - The sample-sheet column `species_dir` is replaced with the `outdir` column which
   represents where the assembly and repeats are downloaded (in immediate sub-directories)
 - Relative paths in the sample-sheet are now evaluated from the `--outdir` parameter
 - Memory usage rules for `samtools dict`
 - Appropriate use of `tabix`'s TBI and CSI indexing, depending on the sequence lengths
+- `--outdir` is a _mandatory_ parameter
 
-### `Added`
+### Parameters
 
-- `--outdir` is a _mandatory_ parameter
+_In the samplesheet_
+
+| Old parameter | New parameter     |
+| ------------- | ----------------- |
+| species_dir   | outdir            |
+
+> **NB:** Parameter has been **updated** if both old and new parameter information is present. </br> **NB:** Parameter has been **added** if just the new parameter information is present. </br> **NB:** Parameter has been **removed** if new parameter information isn't present.
+
+### Software dependencies
+
+Note, since the pipeline is using Nextflow DSL2, each process will be run with its own [Biocontainer](https://biocontainers.pro/#/registry). This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. Only `Docker` or `Singularity` containers are supported, `conda` is not supported.
+
+| Dependency | Old version | New version |
+| ---------- | ----------- | ----------- |
+| multiqc    | 1.13        | 1.14        |
 
-## v1.1.0 - [2022-10-07]
+## [[1.1.0](https://github.com/sanger-tol/insdcdownload/releases/tag/1.1.0)] – Deciduous ent – [2022-10-07]
 
 Minor update that fixes a few bugs
 
@@ -30,7 +47,7 @@ Minor update that fixes a few bugs
 
 - New `species_dir` column to indicate where to download the files to
 
-## v1.0.0 - [2022-08-12]
+## [[1.0.0](https://github.com/sanger-tol/insdcdownload/releases/tag/1.0.0)] – Flaming balrog – [2022-08-12]
 
 Initial release of sanger-tol/insdcdownload, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/CITATION.cff b/CITATION.cff
index 3adf05f..21a2422 100644
--- a/CITATION.cff
+++ b/CITATION.cff
@@ -2,7 +2,7 @@
 # Visit https://bit.ly/cffinit to generate yours today!
 
 cff-version: 1.2.0
-title: sanger-tol/insdcdownload v1.1.0 - Deciduous ent
+title: sanger-tol/insdcdownload
 message: >-
     If you use this software, please cite it using the
     metadata from this file.
@@ -22,5 +22,5 @@ identifiers:
 repository-code: "https://github.com/sanger-tol/insdcdownload"
 license: MIT
 commit: TODO
-version: 1.1.0
+version: 2.0.0
 date-released: "2022-10-07"
diff --git a/nextflow.config b/nextflow.config
index d977225..c281633 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -204,7 +204,7 @@ manifest {
     description     = """Pipeline that downloads assemblies from INSDC into a Tree of Life directory structure"""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=22.10.1'
-    version         = '1.2dev'
+    version         = '2.0.0'
     doi             = '10.5281/zenodo.6983932'
 }
 

From 21c81bf7b83cb1164e2c31078f9ba88fa33f859b Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Fri, 24 May 2024 10:44:11 +0000
Subject: [PATCH 82/85] [prettier]

---
 CHANGELOG.md | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 6d8a09e..8df523a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,9 +20,9 @@ This version supports the new FTP structure of Ensembl
 
 _In the samplesheet_
 
-| Old parameter | New parameter     |
-| ------------- | ----------------- |
-| species_dir   | outdir            |
+| Old parameter | New parameter |
+| ------------- | ------------- |
+| species_dir   | outdir        |
 
 > **NB:** Parameter has been **updated** if both old and new parameter information is present. </br> **NB:** Parameter has been **added** if just the new parameter information is present. </br> **NB:** Parameter has been **removed** if new parameter information isn't present.
 

From 6b71ac3ecea705fe33ae2d4a9bf240a4dfd9ae38 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Mon, 3 Jun 2024 14:33:20 +0100
Subject: [PATCH 83/85] The default branch in main now, PR to main will fail,
 not master

---
 .github/workflows/branch.yml | 16 ++++++++--------
 1 file changed, 8 insertions(+), 8 deletions(-)

diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 9113a81..550c65a 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -1,15 +1,15 @@
 name: nf-core branch protection
-# This workflow is triggered on PRs to master branch on the repository
-# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
+# This workflow is triggered on PRs to main branch on the repository
+# It fails when someone tries to make a PR against the nf-core `main` branch instead of `dev`
 on:
   pull_request_target:
-    branches: [master]
+    branches: [main]
 
 jobs:
   test:
     runs-on: ubuntu-latest
     steps:
-      # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
+      # PRs to the nf-core repo main branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
       - name: Check PRs
         if: github.repository == 'sanger-tol/insdcdownload'
         run: |
@@ -22,7 +22,7 @@ jobs:
         uses: mshick/add-pr-comment@v1
         with:
           message: |
-            ## This PR is against the `master` branch :x:
+            ## This PR is against the `main` branch :x:
 
             * Do not close this PR
             * Click _Edit_ and change the `base` to `dev`
@@ -32,9 +32,9 @@ jobs:
 
             Hi @${{ github.event.pull_request.user.login }},
 
-            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
-            The `master` branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
+            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `main` branch.
+            The `main` branch on nf-core repositories should always contain code from the latest release.
+            Because of this, PRs to `main` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
 
             You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
             Note that even after this, the test will continue to show as failing until you push a new commit.

From 8ee7114f83297b1d7e8c4f8ea78a6d3a4ce387a2 Mon Sep 17 00:00:00 2001
From: Guoying Qi <729395+gq1@users.noreply.github.com>
Date: Mon, 3 Jun 2024 14:37:41 +0100
Subject: [PATCH 84/85] Add one more unchanged file for nf-core linting

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 5a3fa08..442b32c 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -16,6 +16,7 @@ lint:
     - .github/workflows/linting.yml
     - assets/sendmail_template.txt
     - lib/NfcoreTemplate.groovy
+    - .github/workflows/branch.yml
   nextflow_config:
     - manifest.name
     - manifest.homePage

From 89767966c3f0551c044231f03bc4304ec43716ef Mon Sep 17 00:00:00 2001
From: Matthieu Muffato <mm49@sanger.ac.uk>
Date: Tue, 4 Jun 2024 08:10:54 +0000
Subject: [PATCH 85/85] Updated the release date

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8df523a..4a82368 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## [[2.0.0](https://github.com/sanger-tol/insdcdownload/releases/tag/2.0.0)] – Light elf – [2024-05-24]
+## [[2.0.0](https://github.com/sanger-tol/insdcdownload/releases/tag/2.0.0)] – Light elf – [2024-06-04]
 
 This version supports the new FTP structure of Ensembl