From 5e22cd92d1131bab482529775af12c5d624273f7 Mon Sep 17 00:00:00 2001 From: DLBPointon Date: Thu, 4 Jul 2024 19:55:50 +0100 Subject: [PATCH] 2nd Parity update to 1.1.1 treeval --- assets/nf-core-curationpretext_logo_light.png | Bin 9990 -> 0 bytes bin/generate_cram_csv.sh | 91 ++++++++++++++---- docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes .../nf-core-curationpretext_logo_dark.png | Bin 90020 -> 0 bytes .../nf-core-curationpretext_logo_light.png | Bin 89926 -> 0 bytes modules/local/generate_cram_csv.nf | 9 +- subworkflows/local/generate_maps.nf | 2 + 9 files changed, 77 insertions(+), 25 deletions(-) delete mode 100755 assets/nf-core-curationpretext_logo_light.png delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png delete mode 100755 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the CRAM folder +# ><((((°> Y ><((((°> U ><((((°> M ><((((°> I ><((((°> +# Author = yy5 +# ><((((°> Y ><((((°> U ><((((°> M ><((((°> I ><((((°> - ncontainers=$(zcat ${crampath}.crai|wc -l) - base=$(basename $cram .cram) +# Function to process chunking of a CRAM file +chunk_cram() { + local cram=$1 + local chunkn=$2 + local outcsv=$3 + realcram=$(readlink -f ${cram}) + realcrai=$(readlink -f ${cram}.crai) + local rgline=$(samtools view -H "${realcram}" | grep "@RG" | sed 's/\t/\\t/g' | sed "s/'//g") + local ncontainers=$(zcat "${realcrai}" | wc -l) + local base=$(basename "${realcram}" .cram) + local from=0 + local to=10000 - from=0 - to=10000 - - while [ $to -lt $ncontainers ] - do - echo $crampath,${crampath}.crai,${from},${to},${base},${chunkn},${rgline} - from=$((to+1)) - ((to+=10000)) + while [ $to -lt $ncontainers ]; do + echo "${realcram},${realcrai},${from},${to},${base},${chunkn},${rgline}" >> $outcsv + from=$((to + 1)) + ((to += 10000)) ((chunkn++)) done - if [ $from -le $ncontainers ] - then - echo $crampath,${crampath}.crai,${from},${ncontainers},${base},${chunkn},${rgline} + if [ $from -le $ncontainers ]; then + echo "${realcram},${realcrai},${from},${ncontainers},${base},${chunkn},${rgline}" >> $outcsv ((chunkn++)) fi -done + + echo $chunkn +} + +# Function to process a CRAM file +process_cram_file() { + local cram=$1 + local chunkn=$2 + local outcsv=$3 + + local read_groups=$(samtools view -H "$cram" | grep '@RG' | awk '{for(i=1;i<=NF;i++){if($i ~ /^ID:/){print substr($i,4)}}}') + local num_read_groups=$(echo "$read_groups" | wc -w) + + if [ "$num_read_groups" -gt 1 ]; then + # Multiple read groups: process each separately + for rg in $read_groups; do + local output_cram="$(basename "${cram%.cram}")_output_${rg}.cram" + samtools view -h -r "$rg" -o "$output_cram" "$cram" + samtools index "$output_cram" + chunkn=$(chunk_cram "$output_cram" "$chunkn" "$outcsv") + done + else + # Single read group or no read groups + chunkn=$(chunk_cram "$cram" "$chunkn" "$outcsv") + fi + + echo $chunkn +} + +# /\_/\ /\_/\ +# ( o.o ) main ( o.o ) +# > ^ < > ^ < + +# Check if cram_path is provided +if [ -z "$1" ]; then + echo "Usage: $0 " + exit 1 +fi + +cram_path=$1 +chunkn=0 +outcsv=$2 + +# Loop through each CRAM file in the specified directory. cram cannot be the synlinked cram +for cram in ${cram_path}/*.cram; do + realcram=$(readlink -f $cram) + chunkn=$(process_cram_file $realcram $chunkn $outcsv) +done \ No newline at end of file diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png deleted file mode 100755 index 361d0e47acfb424dea1f326590d1eb2f6dfa26b5..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 23458 zcmeFZ2UJtryD!S#x<#o93es(Ww4k)maRbte0-+a?-g^xY-3myTE`8G_KvA54)F1tn})nJ5u%TA4Y;^!^{48eL_}p#q-Umo0M|F1 z74+PQh^X8N|9_jcWbq~ zzn+tZC9B75nKdz=gQ8wo9GJ$P{D~3knlI_`-PRhCw34f1oYDLr^;oEbgxa#A^J%*2 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${prefix}_cram.csv + generate_cram_csv.sh $crampath ${prefix}_cram.csv cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/generate_maps.nf b/subworkflows/local/generate_maps.nf index d6e0b3a..2b55470 100755 --- a/subworkflows/local/generate_maps.nf +++ b/subworkflows/local/generate_maps.nf @@ -48,6 +48,8 @@ workflow GENERATE_MAPS { ) ch_versions = ch_versions.mix( GENERATE_CRAM_CSV.out.versions ) + GENERATE_CRAM_CSV.out.csv.view() + // // LOGIC: make branches for different hic aligner. //