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assembly error for single read R1 #326

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sekhwal opened this issue Nov 12, 2023 · 0 comments
Open

assembly error for single read R1 #326

sekhwal opened this issue Nov 12, 2023 · 0 comments

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@sekhwal
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sekhwal commented Nov 12, 2023

I have aligned a single-read R1 file with a virus genome using bwa. Next step, I am extracting mapped reads from this bam file. I am following these steps.

samtools fastq -F 260 -@ 8 S1_sotred.bam | gzip > S1_mapped.fastq.gz

unicycler -t 8 -s S1_mapped.fastq.gz -o unicycler_S1

However, Unicycler was not able to make any assembly. Please suggest any solution.

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