diff --git a/bindings/experimental/distrdf/python/DistRDF/Backends/Base.py b/bindings/experimental/distrdf/python/DistRDF/Backends/Base.py
index fd266e9d7c92a..1af91f383bd22 100644
--- a/bindings/experimental/distrdf/python/DistRDF/Backends/Base.py
+++ b/bindings/experimental/distrdf/python/DistRDF/Backends/Base.py
@@ -194,6 +194,7 @@ def register_initialization(cls, fun, *args, **kwargs):
             **kwargs (dict): Keyword arguments used to execute the function.
         """
         cls.initialization = partial(fun, *args, **kwargs)
+        fun(*args, **kwargs)
 
     @abstractmethod
     def ProcessAndMerge(self, ranges: List[DataRange],
diff --git a/bindings/experimental/distrdf/test/backend/test_common.py b/bindings/experimental/distrdf/test/backend/test_common.py
index 846cdd35afb20..ef16c6687c270 100644
--- a/bindings/experimental/distrdf/test/backend/test_common.py
+++ b/bindings/experimental/distrdf/test/backend/test_common.py
@@ -58,3 +58,20 @@ def test_header_declaration_on_current_session(self):
         Utils.declare_headers(["test_headers/header4.hxx"])
         self.assertEqual(ROOT.b(1), True)
 
+
+class InitializationTest(unittest.TestCase):
+    """Check the initialize method"""
+
+    def test_initialization_runs_in_current_environment(self):
+        """
+        User initialization method should be executed on the current user
+        session, so actions applied by the user initialization function are
+        also visible in the current scenario.
+        """
+        def defineIntVariable(name, value):
+            import ROOT
+            ROOT.gInterpreter.ProcessLine("int %s = %s;" % (name, value))
+
+        varvalue = 2
+        DistRDF.initialize(defineIntVariable, "myInt", varvalue)
+        self.assertEqual(ROOT.myInt, varvalue)