diff --git a/cover_agent/CoverAgent.py b/cover_agent/CoverAgent.py
index 309cd5e47..36999e683 100644
--- a/cover_agent/CoverAgent.py
+++ b/cover_agent/CoverAgent.py
@@ -30,6 +30,7 @@ def __init__(self, args):
             llm_model=args.model,
             api_base=args.api_base,
             use_report_coverage_feature_flag=args.use_report_coverage_feature_flag,
+            mutation_testing=args.mutation_testing,
         )
 
     def _validate_paths(self):
@@ -89,6 +90,8 @@ def run(self):
             if self.test_gen.current_coverage < (self.test_gen.desired_coverage / 100):
                 self.test_gen.run_coverage()
 
+            self.test_gen.run_mutations()
+
         if self.test_gen.current_coverage >= (self.test_gen.desired_coverage / 100):
             self.logger.info(
                 f"Reached above target coverage of {self.test_gen.desired_coverage}% (Current Coverage: {round(self.test_gen.current_coverage * 100, 2)}%) in {iteration_count} iterations."
diff --git a/cover_agent/PromptBuilder.py b/cover_agent/PromptBuilder.py
index 6b81bf58d..b7205fd66 100644
--- a/cover_agent/PromptBuilder.py
+++ b/cover_agent/PromptBuilder.py
@@ -42,6 +42,7 @@ def __init__(
         additional_instructions: str = "",
         failed_test_runs: str = "",
         language: str = "python",
+        mutation_testing: bool = False,
     ):
         """
         The `PromptBuilder` class is responsible for building a formatted prompt string by replacing placeholders with the actual content of files read during initialization. It takes in various paths and settings as parameters and provides a method to generate the prompt.
@@ -72,6 +73,7 @@ def __init__(
         self.test_file = self._read_file(test_file_path)
         self.code_coverage_report = code_coverage_report
         self.language = language
+        self.mutation_testing = mutation_testing
         # add line numbers to each line in 'source_file'. start from 1
         self.source_file_numbered = "\n".join(
             [f"{i + 1} {line}" for i, line in enumerate(self.source_file.split("\n"))]
@@ -141,12 +143,20 @@ def build_prompt(self) -> dict:
         }
         environment = Environment(undefined=StrictUndefined)
         try:
-            system_prompt = environment.from_string(
-                get_settings().test_generation_prompt.system
-            ).render(variables)
-            user_prompt = environment.from_string(
-                get_settings().test_generation_prompt.user
-            ).render(variables)
+            if self.mutation_testing:
+                system_prompt = environment.from_string(
+                    get_settings().mutation_test_prompt.system
+                ).render(variables)
+                user_prompt = environment.from_string(
+                    get_settings().mutation_test_prompt.user
+                ).render(variables)
+            else:
+                system_prompt = environment.from_string(
+                    get_settings().test_generation_prompt.system
+                ).render(variables)
+                user_prompt = environment.from_string(
+                    get_settings().test_generation_prompt.user
+                ).render(variables)
         except Exception as e:
             logging.error(f"Error rendering prompt: {e}")
             return {"system": "", "user": ""}
diff --git a/cover_agent/UnitTestGenerator.py b/cover_agent/UnitTestGenerator.py
index 01594a8f1..9c861fc42 100644
--- a/cover_agent/UnitTestGenerator.py
+++ b/cover_agent/UnitTestGenerator.py
@@ -3,6 +3,7 @@
 import os
 import re
 import json
+
 from wandb.sdk.data_types.trace_tree import Trace
 
 from cover_agent.Runner import Runner
@@ -14,6 +15,10 @@
 from cover_agent.utils import load_yaml
 from cover_agent.settings.config_loader import get_settings
 
+import subprocess
+
+from shlex import split
+
 
 class UnitTestGenerator:
     def __init__(
@@ -30,6 +35,7 @@ def __init__(
         desired_coverage: int = 90,  # Default to 90% coverage if not specified
         additional_instructions: str = "",
         use_report_coverage_feature_flag: bool = False,
+        mutation_testing: bool = False,
     ):
         """
         Initialize the UnitTestGenerator class with the provided parameters.
@@ -65,6 +71,7 @@ def __init__(
         self.additional_instructions = additional_instructions
         self.language = self.get_code_language(source_file_path)
         self.use_report_coverage_feature_flag = use_report_coverage_feature_flag
+        self.mutation_testing = mutation_testing
         self.last_coverage_percentages = {}
         self.llm_model = llm_model
 
@@ -79,7 +86,7 @@ def __init__(
         self.failed_test_runs = []
         self.total_input_token_count = 0
         self.total_output_token_count = 0
-
+        
         # Run coverage and build the prompt
         self.run_coverage()
         self.prompt = self.build_prompt()
@@ -202,7 +209,7 @@ def run_coverage(self):
                 "Will default to using the full coverage report. You will need to check coverage manually for each passing test."
             )
             with open(self.code_coverage_report_path, "r") as f:
-                self.code_coverage_report = f.read()
+                self.code_coverage_report = f.read()    
 
     @staticmethod
     def get_included_files(included_files):
@@ -739,6 +746,90 @@ def to_dict(self):
     def to_json(self):
         return json.dumps(self.to_dict())
 
+    def run_mutations(self):
+        self.logger.info("Running mutation tests")
+
+        # Run mutation tests
+
+        mutation_prompt_builder = PromptBuilder(
+            source_file_path=self.source_file_path,
+            test_file_path=self.test_file_path,
+            code_coverage_report=self.code_coverage_report,
+            included_files=self.included_files,
+            additional_instructions=self.additional_instructions,
+            failed_test_runs=self.failed_test_runs,
+            language=self.language,
+            mutation_testing=True
+        )
+
+        mutation_prompt = mutation_prompt_builder.build_prompt()
+
+        response, prompt_token_count, response_token_count = (
+            self.ai_caller.call_model(prompt=mutation_prompt)
+        )
+
+        mutation_dict = load_yaml(response)
+
+        for mutation in mutation_dict["mutation"]:
+            result = self.run_mutation(mutation)
+            self.logger.info(f"Mutation result: {result}")
+
+        
+    def run_mutation(self, mutation):
+        mutated_code = mutation.get("mutation", None)
+        line_number = mutation.get("line", None)
+
+         
+        # Read the original content
+        with open(self.source_file_path, "r") as source_file:
+            original_content = source_file.readlines()
+
+        # Determine the indentation level of the line at line_number
+        indentation = len(original_content[line_number]) - len(original_content[line_number].lstrip())
+
+        # Adjust the indentation of the mutated code
+        adjusted_mutated_code = [
+            '    ' * indentation + line if line.strip() else line
+            for line in mutated_code.split("\n")
+        ]
+
+        # Insert the mutated code at the specified spot
+        modified_content = (
+            original_content[:line_number - 1]
+            + adjusted_mutated_code
+            + original_content[line_number:]
+        )
+
+        # Write the modified content back to the file
+        with open(self.source_file_path, "w") as source_file:
+            source_file.writelines(modified_content)
+            source_file.flush()
+
+        # Step 2: Run the test using the Runner class
+        self.logger.info(
+            f'Running test with the following command: "{self.test_command}"'
+        )
+        stdout, stderr, exit_code, time_of_test_command = Runner.run_command(
+            command=self.test_command, cwd=self.test_command_dir
+        )
+
+        try:
+            result = subprocess.run(
+                split(self.test_command),
+                text=True,
+                capture_output=True,
+                cwd=self.test_command_dir,
+                timeout=30,
+            )
+        except Exception as e:
+            logging.error(f"Error running test command: {e}")
+            result = None
+        finally:
+            # Write the modified content back to the file
+            with open(self.source_file_path, "w") as source_file:
+                source_file.writelines(original_content)
+                source_file.flush()
+        return result
 
 def extract_error_message_python(fail_message):
     """
diff --git a/cover_agent/main.py b/cover_agent/main.py
index 1a9a9f10c..fccc8e51e 100644
--- a/cover_agent/main.py
+++ b/cover_agent/main.py
@@ -101,6 +101,11 @@ def parse_args():
         default="",
         help="Path to optional log database. Default: %(default)s.",
     )
+    parser.add_argument(
+        "--mutation-testing",
+        action="store_true",
+        help="Setting this to True enables mutation testing. Default: False.",
+    )
     return parser.parse_args()
 
 
diff --git a/cover_agent/settings/config_loader.py b/cover_agent/settings/config_loader.py
index 1d2622d55..138aef751 100644
--- a/cover_agent/settings/config_loader.py
+++ b/cover_agent/settings/config_loader.py
@@ -7,6 +7,7 @@
     "language_extensions.toml",
     "analyze_suite_test_headers_indentation.toml",
     "analyze_suite_test_insert_line.toml",
+    "mutation_test_prompt.toml",
 ]
 
 
diff --git a/cover_agent/settings/mutation_test_prompt.toml b/cover_agent/settings/mutation_test_prompt.toml
new file mode 100644
index 000000000..61963b41d
--- /dev/null
+++ b/cover_agent/settings/mutation_test_prompt.toml
@@ -0,0 +1,80 @@
+[mutation_test_prompt]
+system="""\
+"""
+
+user="""\
+
+You are an AI mutation testing agent tasked with mutating {{ language }} code to evaluate its robustness.
+
+Mutation Strategy:
+
+1. Logic Tweaks:
+   Modify conditions (e.g., 'if (a < b)' to 'if (a <= b)')
+   Adjust loop boundaries
+   Introduce minor calculation errors
+   Avoid drastic changes or infinite loops.
+
+2. Output Modifications:
+   Change return types or formats
+   Alter response structures
+   Return corrupted or incorrect data
+
+3. Method Interference:
+   Alter function parameters
+   Replace or omit key method calls
+
+4. Failure Injection:
+   Introduce exceptions or error states
+   Simulate system or resource failures
+
+5.Data Handling Faults:
+   Inject parsing errors
+   Bypass data validation
+   Corrupt object states
+
+6. Boundary Condition Testing:
+   Use out-of-bounds indices
+   Test extreme or edge-case parameters
+
+7. Concurrency Issues:
+   Simulate race conditions or deadlocks
+   Introduce timeouts or delays
+
+8. Security Vulnerabilities:
+   Replicate common vulnerabilities (e.g., buffer overflow, SQL injection, XSS)
+   Introduce authentication or authorization bypasses
+
+
+Focus on subtle, realistic mutations that challenge the code's resilience while keeping core functionality intact. Prioritize scenarios likely to arise from programming errors or edge cases.
+
+
+## Source Code to add Mutations to: {{ source_file_name }}
+```{{language}}
+{{ source_file_numbered }}
+```
+
+## Task
+1. Conduct a line-by-line analysis of the source code.
+2. Generate mutations for each test case.
+3. Prioritize mutating function blocks and critical code sections.
+4. Ensure the mutations offer meaningful insights into code quality and test coverage.
+5. Present the output in order of ascending line numbers.
+6. Avoid including manually inserted line numbers in the response.
+7. Limit mutations to single-line changes only.
+
+Example output:
+```yaml
+source: {{source_file}}
+mutation:
+    line: <line number>
+    mutation: |
+      <mutated code>
+```
+
+Use block scalar('|') to format each YAML output.
+
+Response (should be a valid YAML, and nothing else):
+```yaml
+
+Generate mutants that test the code’s resilience while preserving core functionality. Output only in YAML format, with no additional explanations or comments.
+"""
\ No newline at end of file