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shopping list.html
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<section>
<h1 id="Shopping List">Shopping List</h1>
This page contains a compiled list of all parts you will need to buy to build the smfBox, we have split the wavelength dependent components into a separate section:
<ul>
<li><a href="#parts">Standard Parts</a></li>
<li><a href="#wav">Wavelength Dependent Optics</a></li>
</ul>
For a section-by-section description of where each part goes visit the <a href=./overview.html>overview page.</a></li><br><br>
<brb>
To buy all the Thorlabs parts together, click the button below to go to the associated cart on the Thorlabs website. Note that you will still need to buy all the non-Thorlabs parts from their respective vendors. <br>
<div data-thorlabs-code="CARTRETRIEVALCODE"></div> [AWAITING CODE]
<h1 id="-standard parts"><a id="Parts"></a>Standard Parts</h1>
<table>
<thead>
<tr>
<th><strong>Company</strong></th>
<th><strong>Product code</strong></th>
<th><strong>Quantity</strong></th>
<th><strong>Part Nr</strong></th>
</tr>
</thead>
<tbody>
<tr>
<td>Thorlabs</td>
<td>AC254-030-AML</td>
<td>1</td>
<td>L2</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>AC254-100-A-ML</td>
<td>1</td>
<td>L6</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>AC254-75-B-ML</td>
<td>1</td>
<td>L7</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>BB1-E02</td>
<td>2</td>
<td>M1/M2</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>B4CRP/M</td>
<td>1</td>
<td>54</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>C4W</td>
<td>1</td>
<td>53</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>C4W-CC</td>
<td>1</td>
<td>57</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CF125C/M</td>
<td>4</td>
<td>10/21/30/91</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CM1-CC</td>
<td>1</td>
<td>84</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CM1-DCH/M</td>
<td>1</td>
<td>25</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CWY1</td>
<td>1</td>
<td>60</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CXY1</td>
<td>2</td>
<td>78/94</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>CXYZ05/M</td>
<td>1</td>
<td>63</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>DC1-DCH/M</td>
<td>1</td>
<td>72</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>DCC1545M</td>
<td>1</td>
<td>35</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>DET10A/M</td>
<td>1</td>
<td>42</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>DFM1/M</td>
<td>1</td>
<td>85</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ER1.5-P4</td>
<td>1</td>
<td>24</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ER1-P4</td>
<td>1</td>
<td>46</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ER3</td>
<td>1</td>
<td>70</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ER4-P4</td>
<td>1</td>
<td>64</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ER6-P4</td>
<td>1</td>
<td>16</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ERSCA</td>
<td>1</td>
<td>71</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>ERSCA-P4</td>
<td>2</td>
<td>47/64</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>FFM1</td>
<td>1</td>
<td>55</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>KCB1/M</td>
<td>1</td>
<td>58</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>KCB1C/M</td>
<td>2</td>
<td>6/17</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS05P</td>
<td>1</td>
<td>90</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS075P/M</td>
<td>1</td>
<td>88</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS1.5P/M</td>
<td>2</td>
<td>9/20</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS10/M</td>
<td>1</td>
<td>27</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS1P8E</td>
<td>1</td>
<td>29</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS5/M</td>
<td>4</td>
<td>8/19/28/89</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>RS50/M</td>
<td>2</td>
<td>7/18</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SH4S038</td>
<td>1</td>
<td>Screws</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1A1</td>
<td>1</td>
<td>62</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1A12</td>
<td>1</td>
<td>52</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1A3</td>
<td>1</td>
<td>61</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1A9</td>
<td>1</td>
<td>34</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1B3</td>
<td>3</td>
<td>41/80/96</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1CP2</td>
<td>4</td>
<td>56/73/86/87</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1D12C</td>
<td>1</td>
<td>5</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1L03</td>
<td>7</td>
<td>11/22/31/36/40/76/92</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1L03T</td>
<td>1</td>
<td>39</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1L05</td>
<td>4</td>
<td>37/59/66/67</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1L10</td>
<td>5</td>
<td>12/14/15/68/69</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1L20C</td>
<td>1</td>
<td>32</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1P1</td>
<td>1</td>
<td>4</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1S10</td>
<td>1</td>
<td>23</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1T2</td>
<td>4</td>
<td>33/38/77/93</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1T20</td>
<td>1</td>
<td>13</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SM1V10</td>
<td>1</td>
<td>45</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>SML03</td>
<td>2</td>
<td>79/95</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>TR30/M</td>
<td>1</td>
<td>74</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>TR50/M</td>
<td>2</td>
<td>2/44</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>TR50/M0</td>
<td>1</td>
<td>26</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>UPH/30/M</td>
<td>1</td>
<td>75</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>UPH50/M</td>
<td>2</td>
<td>3/43</td>
</tr>
<tr>
<td>Thorlabs</td>
<td>VC3C/M</td>
<td>1</td>
<td>1</td>
</tr>
<tr>
<td>Semrock</td>
<td>MM3-311-t6-1 25.4</td>
<td>1</td>
<td>M3</td>
</tr>
<tr>
<td>RS Components</td>
<td>168-0758</td>
<td>1</td>
<td>22 AWG Hook Up Wire*</td>
</tr>
<tr>
<td>RS Components</td>
<td>526-0241</td>
<td>4</td>
<td>RG-58/U Coaxial Male BNC-Hook Up Wire**</td>
</tr>
<tr>
<td>RS Components</td>
<td>886-0633</td>
<td>4</td>
<td>RG-58 Coaxial Male BNC-Male-BNC</td>
</tr>
<tr>
<td>RS Components</td>
<td>144-0935</td>
<td>4</td>
<td>Coaxial Female BNC-Female BNC Connector</td>
</tr>
<tr>
<td>Piezoconcept</td>
<td>FOC.300</td>
<td>1</td>
<td>50</td>
</tr>
<tr>
<td>Omicron</td>
<td>LightHUB-2</td>
<td>1</td>
<td>515nm and 638nm Lasers</td>
</tr>
<tr>
<td>Olympus</td>
<td>N5218700</td>
<td>1</td>
<td>Objective oil</td>
</tr>
<tr>
<td>Olympus</td>
<td>N1480700</td>
<td>1</td>
<td>49</td>
</tr>
<tr>
<td>Newport</td>
<td>PNH-50</td>
<td>1</td>
<td>P1 (50 µm)</td>
</tr>
<tr>
<td>National Instruments</td>
<td>PCIe-6353</td>
<td>1</td>
<td>PCI card</td>
</tr>
<tr>
<td>National Instruments</td>
<td>186838-02</td>
<td>2</td>
<td>Shielded Cable 68 Pin</td>
</tr>
<tr>
<td>Excelitas</td>
<td>SPCM-AWRH-14</td>
<td>2</td>
<td>82/98</td>
<tr>
<td>Edmund</td>
<td>49792</td>
<td>1</td>
<td>L4</td>
</tr>
</tr>
<tr>
<td>Edmund</td>
<td>49793</td>
<td>2</td>
<td>L3/L5</td>
</tr>
<tr>
<td>Chroma</td>
<td>21012</td>
<td>1</td>
<td>BS1</td>
</tr>
<tr>
<td>Chroma</td>
<td>NC395323-T640lpxr</td>
<td>1</td>
<td>DM2</td>
</tr>
</tbody>
</table>
*Any hook up wire of AWG between 28 - 16 can be used <br>
**Need to strip the Croc Clips </p>
<h1 id="-Wavelength dependent components"><a id="wav"></a> Wavelength Dependent Components</h1>
These are the wavelength dependent components used in the original smfBox, if you would prefer to excite with / detect a different set of wavelengths then that should be possible, but you will need to change these. Note that whilst the 515 laser cannot excite common green fluorophores as efficiently as a 532, it can be more easily digitally modulated without the use of an AOM,
and can also excite fluorophores in the 488 range allowing for a wide choice of labelling. <br>
<table>
<thead>
<tr>
<th><strong>Part Name</strong></th>
<th><strong>Company</strong></th>
<th><strong>Product code</strong></th>
<th><strong>Quantity</strong></th>
<th><strong>Part Nr</strong></th>
</tr>
</thead>
<tbody>
<tr>
<td>Omicron LightHUB®-2 with 515nm 80mW, and 638nm 100mW</td>
<td>Omicron</td>
<td><a href="https://www.omicron-laser.de/english/light-engines/lighthub-laser-combiner/lighthub-laser-combiner.html">LightHUB-2</a></td>
<td>1</td>
<td>Laser</td>
</tr>
<tr>
<td>Excitation Dichroic</td>
<td>Chroma</td>
<td><a href="https://www.chroma.com/products/parts/zt532-640rpc">ZT532/640rpc</a></td>
<td>1</td>
<td>DM1</td>
</tr>
<tr>
<td>Emission Dichroic</td>
<td>Chroma</td>
<td><a href="https://www.chroma.com/products/custom-inventory/nc395323-t640lpxr">NC395323 - T640lpxr</a></td>
<td>1</td>
<td>DM2</td>
</tr>
<tr>
<td>Donor Emission Filter</td>
<td>Semrock</td>
<td><a href="https://www.semrock.com/FilterDetails.aspx?id=FF01-571/72-25">FF01-571/72-25</a></td>
<td>1</td>
<td>F1</td>
</tr>
<tr>
<td>Acceptor Emission Filter</td>
<td>Semrock</td>
<td><a href="https://www.semrock.com/FilterDetails.aspx?id=FF01-679/41-25">FF01-679/41-25</a></td>
<td>1</td>
<td>F2</td>
</tr>
</tbody>
</table>
<figure style="max-width:1000px;">
<img src="images/opticspectra.png" style="mix-blend-mode: multiply; width:90%; padding-left:5%; ">
<figcaption>
<strong>Fig 1a. </strong>Graph showing the absorbance spectra of two typical fluorophores used with the smfBox, the transmission spectrum of the excitation dichroic which reflects the lasers (shown with dotted lines) into the sample, but permits light emitted from the sample.
<strong>1b. </strong>Emission spectra of the same dyes (dark lines), with the filters used to clean up the emission (dotted lines). The spectra of the final light permitted to the APD's is shown in solid blocks. In yellow is the donor emission which reaches the acceptor APD.
</figcaption></figure>
<br>On casual inspection of the above graph it may appear that greater signal from the donor could be acquired with a longer wavelength bandpass filter. It is worth noting that whilst this is true, it would also permit emission of the raman scatter from the 515 laser, so the bandpass filter used was selected specifically to remove this scatter and lower background.
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