You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Transmission electron microscope can produce diffraction image often called SAED. These would be in “1/nm” units since they are from the conjugated plane. When we open such a file (*.dm4) in Fiji with Bio-Format, the following warning is displayed: [WARN] Not adjusting for unknown units: 1/nm
If we go in “scale bar”, the unit was simply replaced with microns, which is misleading and should not be used. I suggest that the 1/nm unit could simply be included as a valid unit in Bio-Format. It's often presented with parenthesis to avoid confusion "(1/nm)".
Here's an exemple of how an analysis of these images could be done. Briefly, length are kept in reciprocal (1/nm) units, then the user would invert them to obtain the d-spacing which is the wanted value is those analysis.
In my case, the camera used to produce the images is a Gatan OneView, filetype is dm4,
Fiji/ImageJ 2.14.0/1.54f; Java 1.8.0_322 [64-bit]; Windows 10
The text was updated successfully, but these errors were encountered:
Thanks, @alexandrebastien. Transferred to the https://github.com/ome/ome-model repo, as this is something that would need to be updated in the OME schema (specifically, the UnitsLength enum). Once the schema is updated, Bio-Formats would be able to make use of the new units more or less automatically.
Transmission electron microscope can produce diffraction image often called SAED. These would be in “1/nm” units since they are from the conjugated plane. When we open such a file (*.dm4) in Fiji with Bio-Format, the following warning is displayed:
[WARN] Not adjusting for unknown units: 1/nm
If we go in “scale bar”, the unit was simply replaced with microns, which is misleading and should not be used. I suggest that the 1/nm unit could simply be included as a valid unit in Bio-Format. It's often presented with parenthesis to avoid confusion "(1/nm)".
Here's an exemple of how an analysis of these images could be done. Briefly, length are kept in reciprocal (1/nm) units, then the user would invert them to obtain the d-spacing which is the wanted value is those analysis.
In my case, the camera used to produce the images is a Gatan OneView, filetype is dm4,
Fiji/ImageJ 2.14.0/1.54f; Java 1.8.0_322 [64-bit]; Windows 10
The text was updated successfully, but these errors were encountered: