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strange bug, two reverse conclusion are shown out at the same run #1732

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dazauzai opened this issue Nov 15, 2024 · 5 comments
Open

strange bug, two reverse conclusion are shown out at the same run #1732

dazauzai opened this issue Nov 15, 2024 · 5 comments
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bug Something isn't working

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@dazauzai
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Description of the bug

Idk why, If I try to delete the two tools which report error, it will report for another problem
-- Check script '/root/.nextflow/assets/nf-core/sarek/main.nf' at line: 342 or see '.nextflow.log' file for more details
I alter my samplesheet for many version. I have tried the sample sheet which only contain normal, tumour, and both. However I still face the same situation whatever I put in the sample sheet. I think I wont because the sample sheet.
samplesheet.csv

Command used and terminal output

/mnt/c/Users/zbjia/Documents/GitHub/sarek# nextflow run nf-core/sarek    -profile docker    --input samplesheet.csv    --outdir /mnt/c/Users/zbjia/Documents/GitHub/sarek/work --wes --intervals /mnt/e/yiming/working/files/bed_file/V3_IDT_03861023_CML_Ph_v3_hg38.bed --tools ascat,cnvkit,controlfreec --fasta /mnt/e/yiming/working/fil
es/reference/GRCh38_masked_v2_decoy_gene.fasta --download_cache -r 3.4.4
 -- Check script '/root/.nextflow/assets/nf-core/sarek/main.nf' at line: 342 or see '.nextflow.log' file for more details
The sample-sheet only contains normal-samples, but the following tools, which were requested with "--tools", expect at least one tumor-sample : ascat, controlfreec
The sample-sheet only contains tumor-samples, but the following tools, which were requested by the option "tools", expect at least one normal-sample : ascat

Relevant files

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System information

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@dazauzai dazauzai added the bug Something isn't working label Nov 15, 2024
@asp8200
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asp8200 commented Nov 15, 2024

I think you may have got a case of this. I would ignore the error messages relating to the sample-sheet and try to look for another error message in .nextflow.log.

@FriederikeHanssen
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Which nextflow version are you using? If you are using 24.10 can you downgrade to the previous stable release?

@dazauzai
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I think you may have got a case of this. I would ignore the error messages relating to the sample-sheet and try to look for another error message in .nextflow.log.

Yes, youre right. I guess the problem is because the problem Missing process or function Channel.empty([[]])
that is strange, I dont know this kind of code, Channel.empty([[]]) why so many [][]

@dazauzai
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Which nextflow version are you using? If you are using 24.10 can you downgrade to the previous stable release?

I tried. I have downgrade it to 24.09.1, But the bug still exsists.

@dazauzai
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您使用的是哪个版本的 nextflow?如果您使用的是 24.10,您可以降级到以前的稳定版本吗?

Which nextflow version are you using? If you are using 24.10 can you downgrade to the previous stable release?
I tried to downgrade it to NXF_VER=24.04.4. now it successfully worked. Thanks very much for your help and your reply. Close this report if you want.

in conclusion, The bug which say that only contain tumour sample or normal sample are not the really problem. The problem is because Channel.empty([[]])
to fix this bug, just downgrade the nextflow to 24.04.4()
just like
NXF_VER=24.04.4 nextflow run nf-core/sarek
thanks again for your help, and hope it will be helpful for whom face the same problem with me.

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