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Allow genotyping germline sites with Mutect 2 #1049

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lbeltrame opened this issue May 30, 2023 · 2 comments
Open

Allow genotyping germline sites with Mutect 2 #1049

lbeltrame opened this issue May 30, 2023 · 2 comments
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enhancement New feature or request

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@lbeltrame
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Description of feature

Some software, like PureCN, expects to have germline sites called along with somatic sites when using MuTect 2. However, currently there's no way to set --genotype-germline-sites true and --genotype-pon-sites true to the caller arguments, or at least not that I've found with a search in the workflows.

This should be optional, and default to off.

Reference: https://bioconductor.org/packages/3.16/bioc/vignettes/PureCN/inst/doc/Quick.html#3_Create_VCF_files

@lbeltrame lbeltrame added the enhancement New feature or request label May 30, 2023
@FriederikeHanssen
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I am not exactly sure, what you want, but if it is running mutect2 with some custom parameters, you can do so easily with a custom config: https://nf-co.re/docs/usage/configuration#understanding-and-modifying-tool-arguments

@lbeltrame
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Thanks, for now that will do. However, in the context of #95 and in particular for PureCN support (where I and @aldosr have been adding the required modules to nf-core to this purpose) this may need to be bolted on to sarek in the future.

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