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wip
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mizraelson committed Oct 31, 2024
1 parent 0934c2c commit 6e5e0a7
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Showing 2 changed files with 52 additions and 52 deletions.
64 changes: 32 additions & 32 deletions src/main/resources/presets/blocks/mitool.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -2,38 +2,38 @@ mitool-consensus-1-steps:
abstract: true
mitool:
consensus:
- parameters:
aAssemblerParameters:
bandWidth: 4
scoring:
type: linear
alphabet: nucleotide
subsMatrix: "simple(match = 5, mismatch = -4)"
gapPenalty: -19
minAlignmentScore: 300
maxNormalizedAlignmentPenalty: 0.30
trimMinimalSumQuality: 0
trimReferenceRegion: true
maxQuality: 45
kMapParameters:
k: 9
useQ1Score: true
coreSequenceKMers: 20
coreSequenceRecordScore: 20
neighbourhoodSize: 2
positiveMinQualityShare: 0.2
positiveMinIntersectionQuality: 300
minAlignmentScoreForDiscardRecord: 0
maxNormalizedAlignmentPenaltyForDiscardRecord: 0.0
minRecordSharePerConsensus: 0.001
minRecordsPerConsensus: 3
useWeightsForRecordsPerConsensus: true
minRecordWeightForUse: 0
maxIterationsPerConsensus: 8
maxConsensuses: 0
saveUnusedReads: true
saveDiscardedReads: true
inferMinRecordsPerConsensus: true
parameters:
aAssemblerParameters:
bandWidth: 4
scoring:
type: linear
alphabet: nucleotide
subsMatrix: "simple(match = 5, mismatch = -4)"
gapPenalty: -19
minAlignmentScore: 300
maxNormalizedAlignmentPenalty: 0.30
trimMinimalSumQuality: 0
trimReferenceRegion: true
maxQuality: 45
kMapParameters:
k: 9
useQ1Score: true
coreSequenceKMers: 20
coreSequenceRecordScore: 20
neighbourhoodSize: 2
positiveMinQualityShare: 0.2
positiveMinIntersectionQuality: 300
minAlignmentScoreForDiscardRecord: 0
maxNormalizedAlignmentPenaltyForDiscardRecord: 0.0
minRecordSharePerConsensus: 0.001
minRecordsPerConsensus: 3
useWeightsForRecordsPerConsensus: true
minRecordWeightForUse: 0
maxIterationsPerConsensus: 8
maxConsensuses: 0
saveUnusedReads: true
saveDiscardedReads: true
inferMinRecordsPerConsensus: true

mitool-consensus-2-steps:
abstract: true
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40 changes: 20 additions & 20 deletions src/main/resources/presets/protocols/bruker.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -49,26 +49,26 @@
consensus:
inheritFrom: mitool-consensus-1-steps
overrides:
- groupingTagTypes:
- Cell
payloadTagTypes:
- Targets
parameters:
maxIterationsPerConsensus: 90
useWeightsForRecordsPerConsensus: false
aAssemblerParameters:
bandWidth: 4
scoring:
alphabet: nucleotide
subsMatrix: "simple(match = 5, mismatch = -10)"
gapPenalty: -19
minAlignmentScore: 60
maxNormalizedAlignmentPenalty: 0.4
positiveMinQualityShare: 0.21
positiveMinIntersectionQuality: 238
minRecordSharePerConsensus: 0.01
minRecordsPerConsensus: 0
maxConsensuses: 0
groupingTagTypes:
- Cell
payloadTagTypes:
- Targets
parameters:
maxIterationsPerConsensus: 90
useWeightsForRecordsPerConsensus: false
aAssemblerParameters:
bandWidth: 4
scoring:
alphabet: nucleotide
subsMatrix: "simple(match = 5, mismatch = -10)"
gapPenalty: -19
minAlignmentScore: 60
maxNormalizedAlignmentPenalty: 0.4
positiveMinQualityShare: 0.21
positiveMinIntersectionQuality: 238
minRecordSharePerConsensus: 0.01
minRecordsPerConsensus: 0
maxConsensuses: 0
align:
inheritFrom: align-single-cell
overrides:
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