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I am Lokeshwaran manoharan, a Bioinformatician by training and currenly working as one at NBIS. I amenthusiastic about computational tools and techniques to answer different biological questions. I have mainly worked with microbiology projects in terms of their ecology and evolution and they always fascinate me.
• PhD in biology from Dec-2011 to Apr-2016 at Lund University, Lund, Sweden.
Dissertation title: Diversity of soil microbial communities in the perspective of targeting functional genes.
• M.S in bioinformatics and systems biology from 2009 to 2011 at Chalmers University of
Technology, Gothenburg, Sweden.
• B.Tech in industrial biotechnology from 2004 to 2008 at Anna University, Chennai, India.
• Research assistant from Aug-2016 to Feb-2016 at Lund University, Lund, Sweden. I worked in
the soil ecology group under my PhD supervisor as a bioinformatician, analyzing the
metratranscriptomics data that was generated in the research group.
• PostDoc position at the Archaea Biology department [Archaea_UniVie](https://twitter.com/Archaea_Vienna/) between April-2017 to January-2019. I mainly worked with archaeal genomics in particular, the Asgard archaea.
Target enrichment strategy for genes in microbial communities
• Developed a method to target functional genes of interest in metagenomic samples.
• Established a pipeline to generate oligonucleotide probes to target genes from communities.
• Validated the method in lab using DNA from soil samples to target enzymatic genes.
Functional diversity in agricultural soils
• Analyzed the diversity of SOM degrading enzymes in different land-use management soils.
• Measured activity of these enzymes through transcriptomics combined with target strategy.
• Measured growth of bacterial and fungal communities using incorporation methods.
Species diversity in agricultural soils
• Analyzed bacterial community composition using 16S rDNA amplicon markers in soils.
• Analyzed fungal communities using ITS (EMF) and 28S (AMF) amplicons in soils.
• Teaching assistant in “2016 Workshop on Genomics” in Cesky Krumlov, Czech Republic.
• Taught in bioinformatics courses such as genomics, metatranscriptomics, PERL and python.
• Taught in ecological courses such as terrestrial ecology and basic molecular genetics.
• Proficiency in PERL, Python, R, Matlab and SQLite. Acquired a great deal of experiences in
variety of genomics/metagenomics and transcriptomics/metatranscriptomics tools.
• Ample experiences with DNA/RNA extraction, PCR, sequence capture, NGS library preparation
and isotope incorporation for microbial growth in soil.
Coming to my real life, I very much like to play volleyball espcially beach and I love movies! I am training in a hobby volleyball team all through the year and we train once/twice per week and I train beach volleyball during the summer more intensively :)
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