diff --git a/.github/actions/setup-poetry/action.yml b/.github/actions/setup-poetry/action.yml
index 2e76676f37792a..a15eb25327ab2b 100644
--- a/.github/actions/setup-poetry/action.yml
+++ b/.github/actions/setup-poetry/action.yml
@@ -8,7 +8,7 @@ inputs:
   poetry-version:
     description: Poetry version to set up
     required: true
-    default: '1.8.4'
+    default: '2.0.1'
   poetry-lockfile:
     description: Path to the Poetry lockfile to restore cache from
     required: true
diff --git a/.github/workflows/api-tests.yml b/.github/workflows/api-tests.yml
index fd98db24b961b4..98075c97cd19fd 100644
--- a/.github/workflows/api-tests.yml
+++ b/.github/workflows/api-tests.yml
@@ -42,25 +42,23 @@ jobs:
         run: poetry install -C api --with dev
 
       - name: Check dependencies in pyproject.toml
-        run: poetry run -C api bash dev/pytest/pytest_artifacts.sh
+        run: poetry run -P api bash dev/pytest/pytest_artifacts.sh
 
       - name: Run Unit tests
-        run: poetry run -C api bash dev/pytest/pytest_unit_tests.sh
+        run: poetry run -P api bash dev/pytest/pytest_unit_tests.sh
 
       - name: Run ModelRuntime
-        run: poetry run -C api bash dev/pytest/pytest_model_runtime.sh
+        run: poetry run -P api bash dev/pytest/pytest_model_runtime.sh
 
       - name: Run dify config tests
-        run: poetry run -C api python dev/pytest/pytest_config_tests.py
+        run: poetry run -P api python dev/pytest/pytest_config_tests.py
 
       - name: Run Tool
-        run: poetry run -C api bash dev/pytest/pytest_tools.sh
+        run: poetry run -P api bash dev/pytest/pytest_tools.sh
 
       - name: Run mypy
         run: |
-          pushd api
-          poetry run python -m mypy --install-types --non-interactive .
-          popd
+          poetry run -C api python -m mypy --install-types --non-interactive .
 
       - name: Set up dotenvs
         run: |
@@ -80,4 +78,4 @@ jobs:
             ssrf_proxy
 
       - name: Run Workflow
-        run: poetry run -C api bash dev/pytest/pytest_workflow.sh
+        run: poetry run -P api bash dev/pytest/pytest_workflow.sh
diff --git a/.github/workflows/style.yml b/.github/workflows/style.yml
index 12213380bdcf52..c80037195a4c97 100644
--- a/.github/workflows/style.yml
+++ b/.github/workflows/style.yml
@@ -38,12 +38,12 @@ jobs:
         if: steps.changed-files.outputs.any_changed == 'true'
         run: |
           poetry run -C api ruff --version
-          poetry run -C api ruff check ./api
-          poetry run -C api ruff format --check ./api
+          poetry run -C api ruff check ./
+          poetry run -C api ruff format --check ./
 
       - name: Dotenv check
         if: steps.changed-files.outputs.any_changed == 'true'
-        run: poetry run -C api dotenv-linter ./api/.env.example ./web/.env.example
+        run: poetry run -P api dotenv-linter ./api/.env.example ./web/.env.example
 
       - name: Lint hints
         if: failure()
diff --git a/.github/workflows/vdb-tests.yml b/.github/workflows/vdb-tests.yml
index 146bee95f21c5f..fab0b8c426a94e 100644
--- a/.github/workflows/vdb-tests.yml
+++ b/.github/workflows/vdb-tests.yml
@@ -70,4 +70,4 @@ jobs:
             tidb
 
       - name: Test Vector Stores
-        run: poetry run -C api bash dev/pytest/pytest_vdb.sh
+        run: poetry run -P api bash dev/pytest/pytest_vdb.sh
diff --git a/api/Dockerfile b/api/Dockerfile
index df676f19261133..fd3532e32d2bf1 100644
--- a/api/Dockerfile
+++ b/api/Dockerfile
@@ -4,7 +4,7 @@ FROM python:3.12-slim-bookworm AS base
 WORKDIR /app/api
 
 # Install Poetry
-ENV POETRY_VERSION=1.8.4
+ENV POETRY_VERSION=2.0.1
 
 # if you located in China, you can use aliyun mirror to speed up
 # RUN pip install --no-cache-dir poetry==${POETRY_VERSION} -i https://mirrors.aliyun.com/pypi/simple/
diff --git a/api/README.md b/api/README.md
index 461dac4759c6c4..6e9f2e8fbb2d57 100644
--- a/api/README.md
+++ b/api/README.md
@@ -79,5 +79,5 @@
 2. Run the tests locally with mocked system environment variables in `tool.pytest_env` section in `pyproject.toml`
 
    ```bash
-   poetry run -C api bash dev/pytest/pytest_all_tests.sh
+   poetry run -P api bash dev/pytest/pytest_all_tests.sh
    ```
diff --git a/api/pyproject.toml b/api/pyproject.toml
index f8c6f599d182b0..ea3dd34475ff2b 100644
--- a/api/pyproject.toml
+++ b/api/pyproject.toml
@@ -1,9 +1,10 @@
 [project]
 name = "dify-api"
 requires-python = ">=3.11,<3.13"
+dynamic = [ "dependencies" ]
 
 [build-system]
-requires = ["poetry-core"]
+requires = ["poetry-core>=2.0.0"]
 build-backend = "poetry.core.masonry.api"
 
 [tool.poetry]
diff --git a/dev/reformat b/dev/reformat
index 94a7f3e6febe50..82f96b8e8f1178 100755
--- a/dev/reformat
+++ b/dev/reformat
@@ -9,10 +9,10 @@ if ! command -v ruff &> /dev/null || ! command -v dotenv-linter &> /dev/null; th
 fi
 
 # run ruff linter
-poetry run -C api ruff check --fix ./api
+poetry run -C api ruff check --fix ./
 
 # run ruff formatter
-poetry run -C api ruff format ./api
+poetry run -C api ruff format ./
 
 # run dotenv-linter linter
-poetry run -C api dotenv-linter ./api/.env.example ./web/.env.example
+poetry run -P api dotenv-linter ./api/.env.example ./web/.env.example
diff --git a/dev/sync-poetry b/dev/sync-poetry
index 23d5d79e900aa3..766382da0120c3 100755
--- a/dev/sync-poetry
+++ b/dev/sync-poetry
@@ -12,7 +12,7 @@ if [ $? -ne 0 ]; then
     # update poetry.lock
     # refreshing lockfile only without updating locked versions
     echo "poetry.lock is outdated, refreshing without updating locked versions ..."
-    poetry lock -C api --no-update
+    poetry lock -C api
 else
   echo "poetry.lock is ready."
 fi